Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)

Elongation of very long-chain fatty acids 4 (Elovl4) is a critical enzyme in the biosynthesis of long-chain and very long-chain polyunsaturated fatty acids (LC- and VLC-PUFAs) in animals. So far, very little is known about the molecular function of Elovl4 in biosynthetic process, especially in fish....

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Detalles Bibliográficos
Autores: Zhao, Nannan, Monroig, Óscar, Navarro, Juan Carlos, Xiang, Xiaojun, Li, Yongnan, Du, Jianlong, Li, Jingqi, Xu, Wei, Mai, Kangsen, Ai, Qinghui
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/202233
Acceso en línea:http://hdl.handle.net/10261/202233
Access Level:acceso abierto
Palabra clave:Elovl4b
Long-chain polyunsaturated fatty acids
Heterologous expression
Lipid sources
Oncorhynchus mykiss
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spelling Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)Zhao, NannanMonroig, ÓscarNavarro, Juan CarlosXiang, XiaojunLi, YongnanDu, JianlongLi, JingqiXu, WeiMai, KangsenAi, QinghuiElovl4bLong-chain polyunsaturated fatty acidsHeterologous expressionLipid sourcesOncorhynchus mykissElongation of very long-chain fatty acids 4 (Elovl4) is a critical enzyme in the biosynthesis of long-chain and very long-chain polyunsaturated fatty acids (LC- and VLC-PUFAs) in animals. So far, very little is known about the molecular function of Elovl4 in biosynthetic process, especially in fish. In this study, two elovl4 paralogs (elovl4b1 and elovl4b2) were successfully cloned from rainbow trout (Oncorhynchus mykiss). The full-length cDNAs of elovl4b1 and elovl4b2 were shown to comprise 1896 bp and 2227 bp, respectively, and both contained 921 bp open reading frames (ORFs), encoded 306 amino acids in high homology. Heterologous expression in yeast showed that rainbow trout Elovl4b1 and Elovl4b2 proteins are able to effectively convert C18-22 polyunsaturated fatty acids (PUFAs) to the polyenoic products up to C32 or C36. Especially, compared with Elovl4b2, Elovl4b1 had much higher efficiency for converting 20:5n-3 and 22:5n-3 to 24:5n-3. Tissue distribution analysis revealed that rainbow trout elovl4b were widely transcribed in various tissues with the highest level in eye, brain and gill. Feeding experiment was conducted with three groups of rainbow trout fed diets with either fish oil (FO) or 100% of the FO replaced by soybean oil (SO) and linseeds oil (LO). Compared to fish fed the diet with FO, elovl4b was significantly up-regulated in the liver of fish fed the diet with SO and in the entire intestine of fish fed the diet with LO, which suggested that elovl4b expression is regulated by dietary lipid sources. These results may contribute to better understand the LC-PUFAs and VLC-PUFAs biosynthetic pathway and the regulation mechanism in rainbow trout.This work was supported by the National Science Fund for Distinguished Young Scholars of China (grant number 31525024) and the Agriculture Research System of China (grant number CARS-47-11).ElsevierNational Thousand Young Talents program of ChinaAgriculture Research System of ChinaMonroig, Óscar [0000-0001-8712-0440]Navarro, Juan Carlos [0000-0001-6976-6686]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/202233reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.aquaculture.2019.734221Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2022332026-05-22T06:33:51Z
dc.title.none.fl_str_mv Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
title Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
spellingShingle Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
Zhao, Nannan
Elovl4b
Long-chain polyunsaturated fatty acids
Heterologous expression
Lipid sources
Oncorhynchus mykiss
title_short Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
title_full Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
title_fullStr Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
title_full_unstemmed Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
title_sort Molecular cloning, functional characterization and nutritional regulation of two elovl4b elongases from rainbow trout (Oncorhynchus mykiss)
dc.creator.none.fl_str_mv Zhao, Nannan
Monroig, Óscar
Navarro, Juan Carlos
Xiang, Xiaojun
Li, Yongnan
Du, Jianlong
Li, Jingqi
Xu, Wei
Mai, Kangsen
Ai, Qinghui
author Zhao, Nannan
author_facet Zhao, Nannan
Monroig, Óscar
Navarro, Juan Carlos
Xiang, Xiaojun
Li, Yongnan
Du, Jianlong
Li, Jingqi
Xu, Wei
Mai, Kangsen
Ai, Qinghui
author_role author
author2 Monroig, Óscar
Navarro, Juan Carlos
Xiang, Xiaojun
Li, Yongnan
Du, Jianlong
Li, Jingqi
Xu, Wei
Mai, Kangsen
Ai, Qinghui
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Thousand Young Talents program of China
Agriculture Research System of China
Monroig, Óscar [0000-0001-8712-0440]
Navarro, Juan Carlos [0000-0001-6976-6686]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Elovl4b
Long-chain polyunsaturated fatty acids
Heterologous expression
Lipid sources
Oncorhynchus mykiss
topic Elovl4b
Long-chain polyunsaturated fatty acids
Heterologous expression
Lipid sources
Oncorhynchus mykiss
description Elongation of very long-chain fatty acids 4 (Elovl4) is a critical enzyme in the biosynthesis of long-chain and very long-chain polyunsaturated fatty acids (LC- and VLC-PUFAs) in animals. So far, very little is known about the molecular function of Elovl4 in biosynthetic process, especially in fish. In this study, two elovl4 paralogs (elovl4b1 and elovl4b2) were successfully cloned from rainbow trout (Oncorhynchus mykiss). The full-length cDNAs of elovl4b1 and elovl4b2 were shown to comprise 1896 bp and 2227 bp, respectively, and both contained 921 bp open reading frames (ORFs), encoded 306 amino acids in high homology. Heterologous expression in yeast showed that rainbow trout Elovl4b1 and Elovl4b2 proteins are able to effectively convert C18-22 polyunsaturated fatty acids (PUFAs) to the polyenoic products up to C32 or C36. Especially, compared with Elovl4b2, Elovl4b1 had much higher efficiency for converting 20:5n-3 and 22:5n-3 to 24:5n-3. Tissue distribution analysis revealed that rainbow trout elovl4b were widely transcribed in various tissues with the highest level in eye, brain and gill. Feeding experiment was conducted with three groups of rainbow trout fed diets with either fish oil (FO) or 100% of the FO replaced by soybean oil (SO) and linseeds oil (LO). Compared to fish fed the diet with FO, elovl4b was significantly up-regulated in the liver of fish fed the diet with SO and in the entire intestine of fish fed the diet with LO, which suggested that elovl4b expression is regulated by dietary lipid sources. These results may contribute to better understand the LC-PUFAs and VLC-PUFAs biosynthetic pathway and the regulation mechanism in rainbow trout.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/202233
url http://hdl.handle.net/10261/202233
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.aquaculture.2019.734221
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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