Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit

More than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal proteins. Alt...

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Authors: Braun, Christina M., Hackert, Philipp, Schmid, Catharina E., Bohnsack, Markus T., Bohnsack, Katherine E., Perez-Fernandez, Jorge
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Universidad de Jaén
Repository:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/6760
Online Access:https://doi.org/10.1093/nar/gkz1079
https://hdl.handle.net/10953/6760
Access Level:Open access
Keyword:ribosome biogenesis
assembly of RNPs
Saccharomyces cerevisiae
Regulation of ribosomal subunit balance
biología molecular
genética
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spelling Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunitBraun, Christina M.Hackert, PhilippSchmid, Catharina E.Bohnsack, Markus T.Bohnsack, Katherine E.Perez-Fernandez, Jorgeribosome biogenesisassembly of RNPsSaccharomyces cerevisiaeRegulation of ribosomal subunit balancebiología moleculargenéticaMore than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal proteins. Although decades of research have provided a wealth of insights into the functions of many AFs, others remain poorly characterized. Pol5 was initially classified with B-type DNA polymerases, however, several lines of evidence indicate the involvement of this protein in ribosome assembly. Here, we show that depletion of Pol5 affects the processing of pre-rRNAs destined for the both the large and small subunits. Furthermore, we identify binding sites for Pol5 in the 5′ external transcribed spacer and within domain III of the 25S rRNA sequence. Consistent with this, we reveal that Pol5 is required for recruitment of ribosomal proteins that form the polypeptide exit tunnel in the LSU and that depletion of Pol5 impairs the release of 5′ ETS fragments from early pre-40S particles. The dual functions of Pol5 in 60S assembly and recycling of pre-40S AFs suggest that this factor could contribute to ensuring the stoichiometric production of ribosomal subunits.‘Deutsche Forschungsgemeinschaft’ in the collaborative research centers [SFB860 to M.T.B., SFB960 AP2 to JPF]Oxford Academic202620262019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://doi.org/10.1093/nar/gkz1079https://hdl.handle.net/10953/6760reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénInglésNucleic Acids Research [2020]; [1]: [405-420]Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/67602026-06-24T12:41:07Z
dc.title.none.fl_str_mv Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
title Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
spellingShingle Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
Braun, Christina M.
ribosome biogenesis
assembly of RNPs
Saccharomyces cerevisiae
Regulation of ribosomal subunit balance
biología molecular
genética
title_short Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
title_full Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
title_fullStr Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
title_full_unstemmed Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
title_sort Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit
dc.creator.none.fl_str_mv Braun, Christina M.
Hackert, Philipp
Schmid, Catharina E.
Bohnsack, Markus T.
Bohnsack, Katherine E.
Perez-Fernandez, Jorge
author Braun, Christina M.
author_facet Braun, Christina M.
Hackert, Philipp
Schmid, Catharina E.
Bohnsack, Markus T.
Bohnsack, Katherine E.
Perez-Fernandez, Jorge
author_role author
author2 Hackert, Philipp
Schmid, Catharina E.
Bohnsack, Markus T.
Bohnsack, Katherine E.
Perez-Fernandez, Jorge
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ribosome biogenesis
assembly of RNPs
Saccharomyces cerevisiae
Regulation of ribosomal subunit balance
biología molecular
genética
topic ribosome biogenesis
assembly of RNPs
Saccharomyces cerevisiae
Regulation of ribosomal subunit balance
biología molecular
genética
description More than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal proteins. Although decades of research have provided a wealth of insights into the functions of many AFs, others remain poorly characterized. Pol5 was initially classified with B-type DNA polymerases, however, several lines of evidence indicate the involvement of this protein in ribosome assembly. Here, we show that depletion of Pol5 affects the processing of pre-rRNAs destined for the both the large and small subunits. Furthermore, we identify binding sites for Pol5 in the 5′ external transcribed spacer and within domain III of the 25S rRNA sequence. Consistent with this, we reveal that Pol5 is required for recruitment of ribosomal proteins that form the polypeptide exit tunnel in the LSU and that depletion of Pol5 impairs the release of 5′ ETS fragments from early pre-40S particles. The dual functions of Pol5 in 60S assembly and recycling of pre-40S AFs suggest that this factor could contribute to ensuring the stoichiometric production of ribosomal subunits.
publishDate 2019
dc.date.none.fl_str_mv 2019
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1093/nar/gkz1079
https://hdl.handle.net/10953/6760
url https://doi.org/10.1093/nar/gkz1079
https://hdl.handle.net/10953/6760
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nucleic Acids Research [2020]; [1]: [405-420]
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford Academic
publisher.none.fl_str_mv Oxford Academic
dc.source.none.fl_str_mv reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
instname:Universidad de Jaén
instname_str Universidad de Jaén
reponame_str RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
collection RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
repository.name.fl_str_mv
repository.mail.fl_str_mv
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