Compact Microfluidic Platform with LED Light‐Actuated Valves for Enzyme‐Linked Immunosorbent Assay Automation
Lab‐on‐a‐chip devices incorporating valves and pumps can perform complex assays involving multiple reagents. However, the instruments used to drive these chips are complex and bulky. In this article, a new wax valve design that uses light from a light emitting diode (LED) for both opening and closin...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/270611 |
| Acceso en línea: | http://hdl.handle.net/10261/270611 https://api.elsevier.com/content/abstract/scopus_id/85129587494 |
| Access Level: | acceso abierto |
| Palabra clave: | Wax valve lab‐on‐a‐chip lab‐on‐a‐foil microfluidic ELISA |
| Sumario: | Lab‐on‐a‐chip devices incorporating valves and pumps can perform complex assays involving multiple reagents. However, the instruments used to drive these chips are complex and bulky. In this article, a new wax valve design that uses light from a light emitting diode (LED) for both opening and closing is reported. The valves and a pumping chamber are integrated in lab‐ona‐foil chips that can be fabricated at low cost using rapid prototyping techniques. A chip for the implementation of enzyme‐linked immunosorbent assays (ELISA) is designed. A porous nitrocellulose material is used for the immobilization of capture antibodies in the microchannel. A compact generic instrument with an array of 64 LEDs, a linear actuator to drive the pumping chamber, and absorbance detection for a colorimetric readout of the assay is also presented. Characterization of all the components and functionalities of the platform and the designed chip demonstrate their potential for assay automation. |
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