Evaluation of the Spanish population coverage of a prospective HLA haplobank of induced pluripotent stem cells

iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive source of HLA-typ...

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Detalhes bibliográficos
Autores: Álvarez Palomo, Belén|||0000-0002-3894-044X, García-Martinez, Iris|||0000-0001-6656-3322, Gayoso, Jorge, Raya, Ángel|||0000-0003-2189-9775, Veiga, Anna|||0000-0002-0943-9904, Abad, María Luisa, Eiras, Adolfo, Guzmán-Fulgencio, María, Luis-Hidalgo, Mar, Eguizábal, Cristina, Santos, Silvia, Balas, Antonio, Alenda, Raquel, Sanchez-Gordo, Francisco, Verdugo, Laura Ponce, Villa, Juliana, Carreras, Enric|||0000-0002-3134-9964, Vidal, Francisco|||0000-0001-8089-4945, Madrigal, Alejandro, Herrero, María José, Rudilla, Francesc, Querol, Sergi|||0000-0002-1105-8633
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:239252
Acesso em linha:https://ddd.uab.cat/record/239252
https://dx.doi.org/urn:doi:10.1186/s13287-021-02301-0
Access Level:acceso abierto
Palavra-chave:Induced pluripotent stem cells
Haplobank
HLA matching
Homozygous
Descrição
Resumo:iPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive source of HLA-typed cells suitable for reprogramming to iPSC. Several initiatives worldwide have been undertaken to create national and international iPSC haplobanks that match a significant part of a population. To create an iPSC haplobank that serves the Spanish population (IPS-PANIA), we have searched the Spanish Bone Marrow Donor Registry (REDMO) to identify the most frequently estimated haplotypes. From the top ten donors identified, we estimated the population coverage using the criteria of zero mismatches in HLA-A, HLA-B, and HLA-DRB1 with different stringencies: high resolution, low resolution, and beneficial mismatch. We have calculated that ten cord blood units from homozygous donors stored at the Spanish cord blood banks can provide HLA-A, HLA-B, and HLA-DRB1 matching for 28.23% of the population. We confirm the feasibility of using banked cord blood units to create an iPSC haplobank that will cover a significant percentage of the Spanish and international population for future advanced therapy replacement strategies. The online version contains supplementary material available at 10.1186/s13287-021-02301-0.

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