Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers

A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226 bp, 434 bp and 106 bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rp...

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Detalles Bibliográficos
Autores: Santos, Carolina S., Ramos, Juliana N., Vieira, Veronica V., Pinheiro, Carina S., Meyer, Roberto, Alcántara Neves, Neuza M., Ramos, Rommel T., Silva, Artur, Hirata, Raphael, Felicori, Liza, Ruiz de Alegría Puig, Carlos, Navas Méndez, Jesús|||0000-0002-8199-9228, Azevedo, Vasco, Mattos Guaraldi, Ana L., Pacheco, Luis G.C.
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/12105
Acceso en línea:http://hdl.handle.net/10902/12105
Access Level:acceso abierto
Palabra clave:Emerging pathogens
Corynebacterium spp
Bacterial identification
Multiplex PCR
Vitek-MS
Descripción
Sumario:A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226 bp, 434 bp and 106 bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.

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