Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p

ObjectiveCirculating extracellular vesicles (EVs) have a great impact on human health as biomarkers and messengers in intercellular signalling. We aimed to determine how the miRNA profile of circulating EVs during an acute coronary event interferes with the vasculogenic potential of endothelial cell...

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Autores: Bobi, Joaquin, Jimenez Trinidad, Francisco Rafael, Ortega Paz, Luis, Cortes Serra, Nuria, Lazaro, Iolanda, Rodríguez Arias, Juan José, Fernandez Becerra, Carmen, Garcia Alvarez, Ana, Sabate, Manel, Brugaletta, Salvatore, Villela Dantas, Ana Paula
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2025
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/219819
Acceso en línea:https://hdl.handle.net/2445/219819
Access Level:acceso abierto
Palabra clave:Malalties coronàries
Angiogènesi
Coronary diseases
Neovascularization
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spelling Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5pBobi, JoaquinJimenez Trinidad, Francisco RafaelOrtega Paz, LuisCortes Serra, NuriaLazaro, IolandaRodríguez Arias, Juan JoséFernandez Becerra, CarmenGarcia Alvarez, AnaSabate, ManelBrugaletta, SalvatoreVillela Dantas, Ana PaulaMalalties coronàriesAngiogènesiCoronary diseasesNeovascularizationObjectiveCirculating extracellular vesicles (EVs) have a great impact on human health as biomarkers and messengers in intercellular signalling. We aimed to determine how the miRNA profile of circulating EVs during an acute coronary event interferes with the vasculogenic potential of endothelial cells (EC). Approach and ResultsEVs were purified from the plasma of patients in the acute phase of non-ST segment elevation myocardial infarction (NSTEMI, n = 33) and from healthy donors (n = 19) used as a control group. Human ECs were treated with EV suspension (5 x 10(7) particles/cm(2)) and tested for their vasculogenic potential and mRNA expression. The EV miRNA profile was determined by miRNA array. EV levels were markedly increased in the plasma of NSTEMI (2.3 x 10(11) +/- 1.5 x 10(10) particles/mL) versus control (1.2 x 10(11) +/- 1.1 x 10(10) particles/mL; p = .02). Treatment of ECs with control EVs increased migration, tube formation, and shaped more branched vessel-like structures in comparison to Sham-treated ECs. Nevertheless, EVs from NSTEMI lacked their vasculogenic potential. Network analysis of EV miRNA and EC mRNA expression revealed a correlation of increased miR-199a-3p and miR-125a-5p expression with a decrease in components involved in EC sprout and stabilization. Combined therapy with miR-199a-3p and miR-125a-5p decreased EC vasculogenic potential. Moreover, anti-miRNA therapy with a combination of anti-miR-125a-5p and anti-miR-199a-3p restored the vasculogenic potential impaired by NSTEMI EVs. ConclusionsCirculating EVs play an important role in the control of angiogenesis. However, in the acute phase of NSTEMI, intercellular communication via EV is modified and loses its ability to generate new blood vessels. The loss of angiogenic capacity of EVs during NSTEMI may be an important player in the disease progression and outcomes.John Wiley & Sons2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/219819Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1111/eci.70022European Journal of Clinical Investigation, 2025https://doi.org/10.1111/eci.70022(c) Stichting European Society for Clinical Investigation Journal Foundation, 2025info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2198192026-05-27T06:46:51Z
dc.title.none.fl_str_mv Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
title Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
spellingShingle Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
Bobi, Joaquin
Malalties coronàries
Angiogènesi
Coronary diseases
Neovascularization
title_short Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
title_full Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
title_fullStr Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
title_full_unstemmed Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
title_sort Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p
dc.creator.none.fl_str_mv Bobi, Joaquin
Jimenez Trinidad, Francisco Rafael
Ortega Paz, Luis
Cortes Serra, Nuria
Lazaro, Iolanda
Rodríguez Arias, Juan José
Fernandez Becerra, Carmen
Garcia Alvarez, Ana
Sabate, Manel
Brugaletta, Salvatore
Villela Dantas, Ana Paula
author Bobi, Joaquin
author_facet Bobi, Joaquin
Jimenez Trinidad, Francisco Rafael
Ortega Paz, Luis
Cortes Serra, Nuria
Lazaro, Iolanda
Rodríguez Arias, Juan José
Fernandez Becerra, Carmen
Garcia Alvarez, Ana
Sabate, Manel
Brugaletta, Salvatore
Villela Dantas, Ana Paula
author_role author
author2 Jimenez Trinidad, Francisco Rafael
Ortega Paz, Luis
Cortes Serra, Nuria
Lazaro, Iolanda
Rodríguez Arias, Juan José
Fernandez Becerra, Carmen
Garcia Alvarez, Ana
Sabate, Manel
Brugaletta, Salvatore
Villela Dantas, Ana Paula
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malalties coronàries
Angiogènesi
Coronary diseases
Neovascularization
topic Malalties coronàries
Angiogènesi
Coronary diseases
Neovascularization
description ObjectiveCirculating extracellular vesicles (EVs) have a great impact on human health as biomarkers and messengers in intercellular signalling. We aimed to determine how the miRNA profile of circulating EVs during an acute coronary event interferes with the vasculogenic potential of endothelial cells (EC). Approach and ResultsEVs were purified from the plasma of patients in the acute phase of non-ST segment elevation myocardial infarction (NSTEMI, n = 33) and from healthy donors (n = 19) used as a control group. Human ECs were treated with EV suspension (5 x 10(7) particles/cm(2)) and tested for their vasculogenic potential and mRNA expression. The EV miRNA profile was determined by miRNA array. EV levels were markedly increased in the plasma of NSTEMI (2.3 x 10(11) +/- 1.5 x 10(10) particles/mL) versus control (1.2 x 10(11) +/- 1.1 x 10(10) particles/mL; p = .02). Treatment of ECs with control EVs increased migration, tube formation, and shaped more branched vessel-like structures in comparison to Sham-treated ECs. Nevertheless, EVs from NSTEMI lacked their vasculogenic potential. Network analysis of EV miRNA and EC mRNA expression revealed a correlation of increased miR-199a-3p and miR-125a-5p expression with a decrease in components involved in EC sprout and stabilization. Combined therapy with miR-199a-3p and miR-125a-5p decreased EC vasculogenic potential. Moreover, anti-miRNA therapy with a combination of anti-miR-125a-5p and anti-miR-199a-3p restored the vasculogenic potential impaired by NSTEMI EVs. ConclusionsCirculating EVs play an important role in the control of angiogenesis. However, in the acute phase of NSTEMI, intercellular communication via EV is modified and loses its ability to generate new blood vessels. The loss of angiogenic capacity of EVs during NSTEMI may be an important player in the disease progression and outcomes.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/219819
url https://hdl.handle.net/2445/219819
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1111/eci.70022
European Journal of Clinical Investigation, 2025
https://doi.org/10.1111/eci.70022
dc.rights.none.fl_str_mv (c) Stichting European Society for Clinical Investigation Journal Foundation, 2025
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Stichting European Society for Clinical Investigation Journal Foundation, 2025
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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