Protocol for triggering plant gene silencing by viral delivery of short RNA
[EN] Virus-induced gene silencing (VIGS) leverages viral vectors to deliver 200-400-nt inserts targeting specific genes. Here, we present a protocol for triggering gene silencing by virus-delivered short RNA inserts (vsRNAi). We describe steps for designing 32-nt vsRNAi that simultaneously target Ni...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/229542 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/229542 |
| Access Level: | acceso abierto |
| Palabra clave: | Biotechnology and bioengineering Microbiology Plant sciences |
| Sumario: | [EN] Virus-induced gene silencing (VIGS) leverages viral vectors to deliver 200-400-nt inserts targeting specific genes. Here, we present a protocol for triggering gene silencing by virus-delivered short RNA inserts (vsRNAi). We describe steps for designing 32-nt vsRNAi that simultaneously target Nicotiana benthamiana homeologous genes involved in chlorophyll biosynthesis and assembling them into JoinTRV, a vector system based on tobacco rattle virus (TRV). This approach results in robust gene silencing phenotypes, including visible leaf yellowing and reduced chlorophyll levels. For complete details on the use and execution of this protocol, please refer to Garc & imath;<acute accent>a et al.1 |
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