Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor

Herrero Lobo, Raquel; Fernández González, Nuria; Marcos Rodrigo, Eva; Martínez, María Alejandra; García Encina, Pedro Antonio; Rodero Raya, María del Rosario; Muñoz Torre, Raúl; Bordel Velasco, Sergio

Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor

Producción Científica

Detalles Bibliográficos
Autores:	Herrero Lobo, Raquel, Fernández González, Nuria, Marcos Rodrigo, Eva, Martínez, María Alejandra, García Encina, Pedro Antonio, Rodero Raya, María del Rosario, Muñoz Torre, Raúl, Bordel Velasco, Sergio
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2024
País:	España
Institución:	Universidad de Valladolid
Repositorio:	UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:	oai:uvadoc.uva.es:10324/75154
Acceso en línea:	https://doi.org/10.1002/jctb.7796 https://uvadoc.uva.es/handle/10324/75154
Access Level:	acceso abierto
Palabra clave:	ectoine hydroxyectoine methanotrophic bacteria Taylor ﬂow methane 3308 Ingeniería y Tecnología del Medio Ambiente

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spelling	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactorHerrero Lobo, RaquelFernández González, NuriaMarcos Rodrigo, EvaMartínez, María AlejandraGarcía Encina, Pedro AntonioRodero Raya, María del RosarioMuñoz Torre, RaúlBordel Velasco, Sergioectoinehydroxyectoinemethanotrophic bacteriaTaylor ﬂowmethane3308 Ingeniería y Tecnología del Medio AmbienteProducción CientíficaBACKGROUND: The production of compatible solutes, such as ectoine and hydroxyectoine, is of great interest due to their industrial and biotechnological applications. Methylomicrobium alcaliphilum was genetically engineered to replace a native gene with a heterologous one, aiming to enhance ectoine production. This study focuses on the optimization of bioreactor conditions to maximize the microbial production of these metabolites from methane. RESULTS: The engineered strain (M. alcaliphilum PstEctD) was cultured in a Taylor flow bioreactor under varying gas recirculation flow rates. Increased flow rates enhanced methane consumption, biomass concentration, and ectoine production. The highest production of ectoine (32 mg/g-VSS) and hydroxyectoine (272 mg/g-VSS) was observed at a flow rate of 0.7 L min−1, while methane removal efficiency improved from 30% to over 60% as flow rates increased. CONCLUSIONS: Optimizing bioreactor conditions, particularly gas recirculation flow rates, significantly improved both the efficiency of methane consumption and the production of ectoine derivatives. This work provides a scalable approach for the sustainable production of compatible solutes from methane, offering potential applications in biotechnological processes utilizing renewable carbon sources. © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).Ministerio de Ciencia, Innovación y Universidades (TED2021-131813B-I00)Ministerio de Ciencia e Innovación (PDC2022-133394-I00)Junta de Castilla y León/FEDER (CLU 2017-09, VA281P18)Unión Europea-NextGenerationEU (Margarita Salas)Argentina-Programa BecArWiley2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1002/jctb.7796https://uvadoc.uva.es/handle/10324/75154reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://scijournals.onlinelibrary.wiley.com/doi/10.1002/jctb.7796info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/751542026-06-13T12:44:47Z
dc.title.none.fl_str_mv	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
title	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
spellingShingle	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor Herrero Lobo, Raquel ectoine hydroxyectoine methanotrophic bacteria Taylor ﬂow methane 3308 Ingeniería y Tecnología del Medio Ambiente
title_short	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
title_full	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
title_fullStr	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
title_full_unstemmed	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
title_sort	Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor
dc.creator.none.fl_str_mv	Herrero Lobo, Raquel Fernández González, Nuria Marcos Rodrigo, Eva Martínez, María Alejandra García Encina, Pedro Antonio Rodero Raya, María del Rosario Muñoz Torre, Raúl Bordel Velasco, Sergio
author	Herrero Lobo, Raquel
author_facet	Herrero Lobo, Raquel Fernández González, Nuria Marcos Rodrigo, Eva Martínez, María Alejandra García Encina, Pedro Antonio Rodero Raya, María del Rosario Muñoz Torre, Raúl Bordel Velasco, Sergio
author_role	author
author2	Fernández González, Nuria Marcos Rodrigo, Eva Martínez, María Alejandra García Encina, Pedro Antonio Rodero Raya, María del Rosario Muñoz Torre, Raúl Bordel Velasco, Sergio
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	ectoine hydroxyectoine methanotrophic bacteria Taylor ﬂow methane 3308 Ingeniería y Tecnología del Medio Ambiente
topic	ectoine hydroxyectoine methanotrophic bacteria Taylor ﬂow methane 3308 Ingeniería y Tecnología del Medio Ambiente
description	Producción Científica
publishDate	2024
dc.date.none.fl_str_mv	2024
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.1002/jctb.7796 https://uvadoc.uva.es/handle/10324/75154
url	https://doi.org/10.1002/jctb.7796 https://uvadoc.uva.es/handle/10324/75154
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	https://scijournals.onlinelibrary.wiley.com/doi/10.1002/jctb.7796
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Wiley
publisher.none.fl_str_mv	Wiley
dc.source.none.fl_str_mv	reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid instname:Universidad de Valladolid
instname_str	Universidad de Valladolid
reponame_str	UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection	UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,811543

Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor

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