Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value

Lyme disease is a tick-borne zoonosis caused by Gram-negative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequ...

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Autores: Grąźlewska, Weronika, Holec-Gąsior, Lucyna, Sołowińska, Karolina, Chmielewski, Tomasz, Fiecek, Beata, Contreras, Marinela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/353530
Acceso en línea:http://hdl.handle.net/10261/353530
Access Level:acceso abierto
Palabra clave:Lyme disease
Chimeric proteins
Borrelia burgdorferi sensu lato
Serodiagnosis
Epitope mapping
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spelling Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic valueGrąźlewska, WeronikaHolec-Gąsior, LucynaSołowińska, KarolinaChmielewski, TomaszFiecek, BeataContreras, MarinelaLyme diseaseChimeric proteinsBorrelia burgdorferi sensu latoSerodiagnosisEpitope mappingLyme disease is a tick-borne zoonosis caused by Gram-negative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequently, two chimeric proteins BmpA-BBK32-M and BmpA-BBK32-G were designed to validate the construction of chimeras using the identified epitopes for the detection of IgM and IgG, respectively, by ELISA. IgG-ELISA based on the BmpA-BBK32-G antigen showed 71% sensitivity and 95% specificity, whereas a slightly lower diagnostic utility was obtained for IgM-ELISA based on BmpA-BBK32-M, where the sensitivity was also 71% but the specificity decreased to 89%. The reactivity of chimeric proteins with nondedicated antibodies was much lower. These results suggest that the identified epitopes may be useful in the design of new forms of antigens to increase the effectiveness of Lyme disease serodiagnosis. It has also been proven that appropriate selection of epitopes enables the construction of chimeric proteins exhibiting reactivity with a specific antibody isotype.This study was supported by the additional budget of the grant IJC2020-042710 by the Ministerio de Ciencia, Innovación y Universidades Spain. Marinela Contreras is funded by the same grant.Peer reviewedAmerican Chemical SocietyMinisterio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/353530reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//IJC2020-042710Grąźlewska, Weronika; Holec-Gąsior, Lucyna; Sołowińska, Karolina; Chmielewski, Tomasz; Fiecek, Beata; Contreras, Marinela; 2023; Supplementary Information: Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value [Dataset]; American Chemical Society; https://doi.org/10.1021/acsinfecdis.3c00258https://doi.org/10.1021/acsinfecdis.3c00258Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3535302026-05-22T06:33:51Z
dc.title.none.fl_str_mv Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
title Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
spellingShingle Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
Grąźlewska, Weronika
Lyme disease
Chimeric proteins
Borrelia burgdorferi sensu lato
Serodiagnosis
Epitope mapping
title_short Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
title_full Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
title_fullStr Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
title_full_unstemmed Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
title_sort Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value
dc.creator.none.fl_str_mv Grąźlewska, Weronika
Holec-Gąsior, Lucyna
Sołowińska, Karolina
Chmielewski, Tomasz
Fiecek, Beata
Contreras, Marinela
author Grąźlewska, Weronika
author_facet Grąźlewska, Weronika
Holec-Gąsior, Lucyna
Sołowińska, Karolina
Chmielewski, Tomasz
Fiecek, Beata
Contreras, Marinela
author_role author
author2 Holec-Gąsior, Lucyna
Sołowińska, Karolina
Chmielewski, Tomasz
Fiecek, Beata
Contreras, Marinela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lyme disease
Chimeric proteins
Borrelia burgdorferi sensu lato
Serodiagnosis
Epitope mapping
topic Lyme disease
Chimeric proteins
Borrelia burgdorferi sensu lato
Serodiagnosis
Epitope mapping
description Lyme disease is a tick-borne zoonosis caused by Gram-negative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequently, two chimeric proteins BmpA-BBK32-M and BmpA-BBK32-G were designed to validate the construction of chimeras using the identified epitopes for the detection of IgM and IgG, respectively, by ELISA. IgG-ELISA based on the BmpA-BBK32-G antigen showed 71% sensitivity and 95% specificity, whereas a slightly lower diagnostic utility was obtained for IgM-ELISA based on BmpA-BBK32-M, where the sensitivity was also 71% but the specificity decreased to 89%. The reactivity of chimeric proteins with nondedicated antibodies was much lower. These results suggest that the identified epitopes may be useful in the design of new forms of antigens to increase the effectiveness of Lyme disease serodiagnosis. It has also been proven that appropriate selection of epitopes enables the construction of chimeric proteins exhibiting reactivity with a specific antibody isotype.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/353530
url http://hdl.handle.net/10261/353530
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//IJC2020-042710
Grąźlewska, Weronika; Holec-Gąsior, Lucyna; Sołowińska, Karolina; Chmielewski, Tomasz; Fiecek, Beata; Contreras, Marinela; 2023; Supplementary Information: Epitope mapping of BmpA and BBK32 Borrelia burgdorferi sensu stricto antigens for the design of chimeric proteins with potential diagnostic value [Dataset]; American Chemical Society; https://doi.org/10.1021/acsinfecdis.3c00258
https://doi.org/10.1021/acsinfecdis.3c00258
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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