Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy
Idiopathic dilated cardiomyopathy () is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low-density lipoprotein receptor-related protein 1 (1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels...
| Autores: | , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:186253 |
| Acceso en línea: | https://ddd.uab.cat/record/186253 https://dx.doi.org/urn:doi:10.1111/jcmm.13211 |
| Access Level: | acceso abierto |
| Palabra clave: | Biomarker Idiopathic dilated cardiomyopathy Extracellular vesicles Slrp1 Size-exclusion chromatography |
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Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathyRudilla, F..Gálvez-Montón, Carolina|||0000-0003-2254-9371Gonzalo Calvo, David de|||0000-0003-2240-3532Valero, Ana GámezGastelurrutia, Paloma|||0000-0001-6974-9210Revuelta-López, Elena|||0000-0001-8962-5936Prat-Vidal, Cristina|||0000-0002-5621-1101Soler Botija, CarolinaLlucià-Valldeperas, AidaPerea Gil, IsaacIborra Egea, Oriol|||0000-0002-7280-859XBorràs i Serres, Francesc Enric|||0000-0003-4038-1912Lupón, Josep|||0000-0002-5601-9611Llorente-Cortés, Vicenta|||0000-0002-0067-7201Bayés-Genís, Antoni|||0000-0002-3044-197XBiomarkerIdiopathic dilated cardiomyopathyExtracellular vesiclesSlrp1Size-exclusion chromatographyIdiopathic dilated cardiomyopathy () is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low-density lipoprotein receptor-related protein 1 (1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels of soluble 1 (1) in patients and healthy controls and elucidated whether 1 is exported out of the myocardium through extracellular vesicles (s) to gain a better understanding of the pathogenesis of the disease. 1 α chain expression was analysed in samples collected from the left ventricles of explanted hearts using immunohistochemistry. 1 concentrations were determined in platelet-free plasma by enzyme-linked immunosorbent assay. Plasma-derived s were extracted by size-exclusion chromatography () and characterized by nanoparticle tracking analysis and cryo-transmission electron microscopy. The distributions of vesicular (9, 81) and myocardial (caveolin-3) proteins and 1 α chain were assessed in fractions by flow cytometry. 1 α chain was preferably localized to blood vessels in compared to control myocardium. Circulating 1 was increased in patients. 9- and 81-positive fractions enriched with membrane vesicles with the expected size and morphology were isolated from both groups. The 1 α chain was not present in these fractions, which were also positive for caveolin-3. The increase in circulating 1 in patients may be clinically valuable. Although s do not contribute to higher 1 levels in , a comprehensive analysis of content would provide further insights into the search for novel blood markers.Universitat Autònoma de Barcelona 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/186253https://dx.doi.org/urn:doi:10.1111/jcmm.13211reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2014-59892-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 JCI-2012-14025Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FISPI14-01682Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FISPI14-01729Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CD14-00109open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1862532026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
| title |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
| spellingShingle |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy Rudilla, F.. Biomarker Idiopathic dilated cardiomyopathy Extracellular vesicles Slrp1 Size-exclusion chromatography |
| title_short |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
| title_full |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
| title_fullStr |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
| title_full_unstemmed |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
| title_sort |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
| dc.creator.none.fl_str_mv |
Rudilla, F.. Gálvez-Montón, Carolina|||0000-0003-2254-9371 Gonzalo Calvo, David de|||0000-0003-2240-3532 Valero, Ana Gámez Gastelurrutia, Paloma|||0000-0001-6974-9210 Revuelta-López, Elena|||0000-0001-8962-5936 Prat-Vidal, Cristina|||0000-0002-5621-1101 Soler Botija, Carolina Llucià-Valldeperas, Aida Perea Gil, Isaac Iborra Egea, Oriol|||0000-0002-7280-859X Borràs i Serres, Francesc Enric|||0000-0003-4038-1912 Lupón, Josep|||0000-0002-5601-9611 Llorente-Cortés, Vicenta|||0000-0002-0067-7201 Bayés-Genís, Antoni|||0000-0002-3044-197X |
| author |
Rudilla, F.. |
| author_facet |
Rudilla, F.. Gálvez-Montón, Carolina|||0000-0003-2254-9371 Gonzalo Calvo, David de|||0000-0003-2240-3532 Valero, Ana Gámez Gastelurrutia, Paloma|||0000-0001-6974-9210 Revuelta-López, Elena|||0000-0001-8962-5936 Prat-Vidal, Cristina|||0000-0002-5621-1101 Soler Botija, Carolina Llucià-Valldeperas, Aida Perea Gil, Isaac Iborra Egea, Oriol|||0000-0002-7280-859X Borràs i Serres, Francesc Enric|||0000-0003-4038-1912 Lupón, Josep|||0000-0002-5601-9611 Llorente-Cortés, Vicenta|||0000-0002-0067-7201 Bayés-Genís, Antoni|||0000-0002-3044-197X |
| author_role |
author |
| author2 |
Gálvez-Montón, Carolina|||0000-0003-2254-9371 Gonzalo Calvo, David de|||0000-0003-2240-3532 Valero, Ana Gámez Gastelurrutia, Paloma|||0000-0001-6974-9210 Revuelta-López, Elena|||0000-0001-8962-5936 Prat-Vidal, Cristina|||0000-0002-5621-1101 Soler Botija, Carolina Llucià-Valldeperas, Aida Perea Gil, Isaac Iborra Egea, Oriol|||0000-0002-7280-859X Borràs i Serres, Francesc Enric|||0000-0003-4038-1912 Lupón, Josep|||0000-0002-5601-9611 Llorente-Cortés, Vicenta|||0000-0002-0067-7201 Bayés-Genís, Antoni|||0000-0002-3044-197X |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Biomarker Idiopathic dilated cardiomyopathy Extracellular vesicles Slrp1 Size-exclusion chromatography |
| topic |
Biomarker Idiopathic dilated cardiomyopathy Extracellular vesicles Slrp1 Size-exclusion chromatography |
| description |
Idiopathic dilated cardiomyopathy () is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low-density lipoprotein receptor-related protein 1 (1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels of soluble 1 (1) in patients and healthy controls and elucidated whether 1 is exported out of the myocardium through extracellular vesicles (s) to gain a better understanding of the pathogenesis of the disease. 1 α chain expression was analysed in samples collected from the left ventricles of explanted hearts using immunohistochemistry. 1 concentrations were determined in platelet-free plasma by enzyme-linked immunosorbent assay. Plasma-derived s were extracted by size-exclusion chromatography () and characterized by nanoparticle tracking analysis and cryo-transmission electron microscopy. The distributions of vesicular (9, 81) and myocardial (caveolin-3) proteins and 1 α chain were assessed in fractions by flow cytometry. 1 α chain was preferably localized to blood vessels in compared to control myocardium. Circulating 1 was increased in patients. 9- and 81-positive fractions enriched with membrane vesicles with the expected size and morphology were isolated from both groups. The 1 α chain was not present in these fractions, which were also positive for caveolin-3. The increase in circulating 1 in patients may be clinically valuable. Although s do not contribute to higher 1 levels in , a comprehensive analysis of content would provide further insights into the search for novel blood markers. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2 2017-01-01 2017 2017-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/186253 https://dx.doi.org/urn:doi:10.1111/jcmm.13211 |
| url |
https://ddd.uab.cat/record/186253 https://dx.doi.org/urn:doi:10.1111/jcmm.13211 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SAF2014-59892-R Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 JCI-2012-14025 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FISPI14-01682 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FISPI14-01729 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CD14-00109 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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