Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations

Bacteriocin production in Lactobacillus plantarum NC8 is activated by coculture with specific bacteriocin production-inducing bacterial strains. The system is further regulated by a three-component regulatory system involving a specific autoinducer peptide (PLNC8IF). We have used L. plantarum NC8 as...

Descripción completa

Detalles Bibliográficos
Autores: Ruiz-Barba, José Luis, Caballero-Guerrero, Belén, Maldonado-Barragán, Antonio, Jiménez Díaz, Rufino
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/23840
Acceso en línea:http://hdl.handle.net/10261/23840
Access Level:acceso abierto
Palabra clave:Lactobacillus plantarum
Bacteriocin
Induction
Coculture
Fermentation
id ES_f0b134c8c1e32fdcd4833c33209bfc8e
oai_identifier_str oai:digital.csic.es:10261/23840
network_acronym_str ES
network_name_str España
repository_id_str
spelling Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentationsRuiz-Barba, José LuisCaballero-Guerrero, BelénMaldonado-Barragán, AntonioJiménez Díaz, RufinoLactobacillus plantarumBacteriocinInductionCocultureFermentationBacteriocin production in Lactobacillus plantarum NC8 is activated by coculture with specific bacteriocin production-inducing bacterial strains. The system is further regulated by a three-component regulatory system involving a specific autoinducer peptide (PLNC8IF). We have used L. plantarum NC8 as a starter culture in Spanish-style green olive fermentations and examined the influence of coculturing in its survival. We found that L. plantarum NC8 greatly enhanced its growth and survival in the olive fermentations when coinoculated with two specific bacteriocin-production inducing strains, i.e. Enterococcus faecium 6T1a-20 and Pediococcus pentosaceus FBB63, when compared to singly-inoculated fermentations. In addition, a constitutive bacteriocin-producer NC8-derivative strain was used as a control in the olive fermentations and showed also better viability than the parental NC8 strain. Our results suggest the involvement of bacteriocin production in the viability enhancement found in both cases. We postulate that the presence of specific bacteria is recognized by L. plantarum NC8 as an environmental stimulus to switch a specific adaptive response on, most probably involving bacteriocin production. The design of novel bacteriocin-producing starter cultures for food fermentations should consider their constitutive versus regulated character.This work was supported by the Spanish Ministry of Education and Science (MEC) and the Spanish Ministry of Science and Innovation (MICINN) through the Projects AGL2006-00763 and AGL2009-07861, respectively. A.M.-B. was the recipient of a grant from MEC-MCYT (FPI Programme). We want to express our gratitude to Mrs. Elena Cabello Herrera and Mr. Francisco Sánchez Roldán, from the Instituto de la Grasa (CSIC, Sevilla) for their skilful technical assistance.Peer reviewed201020102010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501219648 bytesapplication/pdfhttp://hdl.handle.net/10261/23840reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.fm.2009.10.002info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/238402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
title Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
spellingShingle Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
Ruiz-Barba, José Luis
Lactobacillus plantarum
Bacteriocin
Induction
Coculture
Fermentation
title_short Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
title_full Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
title_fullStr Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
title_full_unstemmed Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
title_sort Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations
dc.creator.none.fl_str_mv Ruiz-Barba, José Luis
Caballero-Guerrero, Belén
Maldonado-Barragán, Antonio
Jiménez Díaz, Rufino
author Ruiz-Barba, José Luis
author_facet Ruiz-Barba, José Luis
Caballero-Guerrero, Belén
Maldonado-Barragán, Antonio
Jiménez Díaz, Rufino
author_role author
author2 Caballero-Guerrero, Belén
Maldonado-Barragán, Antonio
Jiménez Díaz, Rufino
author2_role author
author
author
dc.subject.none.fl_str_mv Lactobacillus plantarum
Bacteriocin
Induction
Coculture
Fermentation
topic Lactobacillus plantarum
Bacteriocin
Induction
Coculture
Fermentation
description Bacteriocin production in Lactobacillus plantarum NC8 is activated by coculture with specific bacteriocin production-inducing bacterial strains. The system is further regulated by a three-component regulatory system involving a specific autoinducer peptide (PLNC8IF). We have used L. plantarum NC8 as a starter culture in Spanish-style green olive fermentations and examined the influence of coculturing in its survival. We found that L. plantarum NC8 greatly enhanced its growth and survival in the olive fermentations when coinoculated with two specific bacteriocin-production inducing strains, i.e. Enterococcus faecium 6T1a-20 and Pediococcus pentosaceus FBB63, when compared to singly-inoculated fermentations. In addition, a constitutive bacteriocin-producer NC8-derivative strain was used as a control in the olive fermentations and showed also better viability than the parental NC8 strain. Our results suggest the involvement of bacteriocin production in the viability enhancement found in both cases. We postulate that the presence of specific bacteria is recognized by L. plantarum NC8 as an environmental stimulus to switch a specific adaptive response on, most probably involving bacteriocin production. The design of novel bacteriocin-producing starter cultures for food fermentations should consider their constitutive versus regulated character.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/23840
url http://hdl.handle.net/10261/23840
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.fm.2009.10.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 219648 bytes
application/pdf
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424005953355776
score 15.812429

Similares en LA Referencia