Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein

The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN-γ (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided i...

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Authors: Martínez-Orellana, Pamela, González, Noemí, Baldassarre, Antonella, Álvarez-Fernández, Alejandra, Ordeix, Laura, Paradies, Paola, Soto Álvarez, Manuel, Solano-Gallego, Laia
Format: article
Publication Date:2022
Country:España
Institution:Universidad Autónoma de Madrid
Repository:Biblos-e Archivo. Repositorio Institucional de la UAM
Language:English
OAI Identifier:oai:repositorio.uam.es:10486/706531
Online Access:http://hdl.handle.net/10486/706531
https://dx.doi.org/10.3390/vetsci9030116
Access Level:Open access
Keyword:Antibody
Antigen
Gamma interferon
Leishmania infantum soluble antigen
Recombinant protein
Biología y Biomedicina / Biología
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spelling Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant proteinMartínez-Orellana, PamelaGonzález, NoemíBaldassarre, AntonellaÁlvarez-Fernández, AlejandraOrdeix, LauraParadies, PaolaSoto Álvarez, ManuelSolano-Gallego, LaiaAntibodyAntigenGamma interferonLeishmania infantum soluble antigenRecombinant proteinBiología y Biomedicina / BiologíaThe effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN-γ (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0.001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0.0001). All groups showed higher IFN-γ levels after LSA compared to rKMP11 responses (p < 0.05). The highest response to LSA was found in Ibizan hounds (p < 0.05). IFN-γ to LSA and rKMP11 stimulation was observed in 34% and in 2.8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN-γ production was more intense to LSA than stimulated to rKMP11This research was funded by Spanish ministry grant, Ministerio de Economía y competitividad and Fondo Europeo de Desarrollo Regional (FEDER, EU) (AGL2015-68477)MDPIDepartamento de Biología MolecularFacultad de Ciencias20222022-03-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/706531https://dx.doi.org/10.3390/vetsci9030116reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7065312026-06-23T12:46:27Z
dc.title.none.fl_str_mv Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
title Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
spellingShingle Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
Martínez-Orellana, Pamela
Antibody
Antigen
Gamma interferon
Leishmania infantum soluble antigen
Recombinant protein
Biología y Biomedicina / Biología
title_short Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
title_full Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
title_fullStr Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
title_full_unstemmed Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
title_sort Humoral responses and ex vivo IFN-γ production after canine whole blood stimulation with Leishmania infantum antigen or KMP11 recombinant protein
dc.creator.none.fl_str_mv Martínez-Orellana, Pamela
González, Noemí
Baldassarre, Antonella
Álvarez-Fernández, Alejandra
Ordeix, Laura
Paradies, Paola
Soto Álvarez, Manuel
Solano-Gallego, Laia
author Martínez-Orellana, Pamela
author_facet Martínez-Orellana, Pamela
González, Noemí
Baldassarre, Antonella
Álvarez-Fernández, Alejandra
Ordeix, Laura
Paradies, Paola
Soto Álvarez, Manuel
Solano-Gallego, Laia
author_role author
author2 González, Noemí
Baldassarre, Antonella
Álvarez-Fernández, Alejandra
Ordeix, Laura
Paradies, Paola
Soto Álvarez, Manuel
Solano-Gallego, Laia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Antibody
Antigen
Gamma interferon
Leishmania infantum soluble antigen
Recombinant protein
Biología y Biomedicina / Biología
topic Antibody
Antigen
Gamma interferon
Leishmania infantum soluble antigen
Recombinant protein
Biología y Biomedicina / Biología
description The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN-γ (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0.001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0.0001). All groups showed higher IFN-γ levels after LSA compared to rKMP11 responses (p < 0.05). The highest response to LSA was found in Ibizan hounds (p < 0.05). IFN-γ to LSA and rKMP11 stimulation was observed in 34% and in 2.8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN-γ production was more intense to LSA than stimulated to rKMP11
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-03-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/706531
https://dx.doi.org/10.3390/vetsci9030116
url http://hdl.handle.net/10486/706531
https://dx.doi.org/10.3390/vetsci9030116
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423961680379904
score 15,300724

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