Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation

Introduction: measuring the chemokine CXCL9 in urine by enzyme-linked immunosorbent assay (ELISA) can diagnose acute cellular rejection (ACR) noninvasively after kidney transplantation, but the required 12- to 24-hour turnaround time is not ideal for rapid, clinical decision-making. Methods: we deve...

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Autores: Gandolfini, Ilaria, Harris, Cynthia, Abecassis, Michael, Anderson, Lisa, Bestard Matamoros, Oriol, Comai, Giorgia, Cravedi, Paolo, Cremaschi, Elena, Duty, J. Andrew, Florman, Sander, Friedewald, John, La Manna, Gaetano, Maggiore, Umberto, Moran, Thomas, Piotti, Giovanni, Purroy, Carolina, Jarque, Marta, Nair, Vinay, Shapiro, Ron, Reid-Adam, Jessica, Heeger, Peter S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/133090
Acceso en línea:https://hdl.handle.net/2445/133090
Access Level:acceso abierto
Palabra clave:Rebuig (Biologia)
Quimiocines
Trasplantament renal
Interferometria
Graft rejection
Chemokines
Kidney transplantation
Interferometry
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spelling Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantationGandolfini, IlariaHarris, CynthiaAbecassis, MichaelAnderson, LisaBestard Matamoros, OriolComai, GiorgiaCravedi, PaoloCremaschi, ElenaDuty, J. AndrewFlorman, SanderFriedewald, JohnLa Manna, GaetanoMaggiore, UmbertoMoran, ThomasPiotti, GiovanniPurroy, CarolinaJarque, MartaNair, VinayShapiro, RonReid-Adam, JessicaHeeger, Peter S.Rebuig (Biologia)QuimiocinesTrasplantament renalInterferometriaGraft rejectionChemokinesKidney transplantationInterferometryIntroduction: measuring the chemokine CXCL9 in urine by enzyme-linked immunosorbent assay (ELISA) can diagnose acute cellular rejection (ACR) noninvasively after kidney transplantation, but the required 12- to 24-hour turnaround time is not ideal for rapid, clinical decision-making. Methods: we developed a biolayer interferometry (BLI)−based assay to rapidly measure urinary CXCL9 in <1 hour. We validated this new assay versus standard ELISA in 86 urine samples from kidney transplantation recipients with various diagnoses. We then used BLI to analyze samples from 56 kidney transplantation recipients, including 46 subjects who experienced an acute rise in serum creatinine associated with biopsy-proven ACR (n = 22), subclinical rejection (n = 15), or no infiltrates (n = 9), and 10 stable kidney transplantation recipients with surveillance biopsies. To assess its usefulness in detecting adequacy of therapy we serially measured serum creatinine and urinary CXCL9 in 6 subjects after treatment for ACR, and correlated the results with histological diagnoses on follow-up biopsies. Results: BLI accurately and reproducibly detected urinary CXCL9 in <1 hour. BLI-based results showed that urinary CXCL9 was >200 pg/ml in subjects with ACR and ≤100 pg/ml in subjects with stable kidney function without cellular infiltrates. In samples obtained after treatment for ACR, BLI CXCL9 measurements detected biopsy-proven intragraft infiltrates despite treatment-induced reduction in serum creatinine. Discussion: together, our proof-of-principle results demonstrate that BLI-based urinary CXCL9 detection has potential as a point-of-care noninvasive biomarker to diagnose and guide therapy for ACR in kidney transplantation recipients.Elsevier2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/133090Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.ekir.2017.06.010Kidney International Reports, 2017, vol. 2, num. 6, p. 1186-1193https://doi.org/10.1016/j.ekir.2017.06.010cc-by-nc-nd (c) International Society of Nephrology, 2017http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1330902026-05-27T06:46:51Z
dc.title.none.fl_str_mv Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
title Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
spellingShingle Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
Gandolfini, Ilaria
Rebuig (Biologia)
Quimiocines
Trasplantament renal
Interferometria
Graft rejection
Chemokines
Kidney transplantation
Interferometry
title_short Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
title_full Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
title_fullStr Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
title_full_unstemmed Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
title_sort Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
dc.creator.none.fl_str_mv Gandolfini, Ilaria
Harris, Cynthia
Abecassis, Michael
Anderson, Lisa
Bestard Matamoros, Oriol
Comai, Giorgia
Cravedi, Paolo
Cremaschi, Elena
Duty, J. Andrew
Florman, Sander
Friedewald, John
La Manna, Gaetano
Maggiore, Umberto
Moran, Thomas
Piotti, Giovanni
Purroy, Carolina
Jarque, Marta
Nair, Vinay
Shapiro, Ron
Reid-Adam, Jessica
Heeger, Peter S.
author Gandolfini, Ilaria
author_facet Gandolfini, Ilaria
Harris, Cynthia
Abecassis, Michael
Anderson, Lisa
Bestard Matamoros, Oriol
Comai, Giorgia
Cravedi, Paolo
Cremaschi, Elena
Duty, J. Andrew
Florman, Sander
Friedewald, John
La Manna, Gaetano
Maggiore, Umberto
Moran, Thomas
Piotti, Giovanni
Purroy, Carolina
Jarque, Marta
Nair, Vinay
Shapiro, Ron
Reid-Adam, Jessica
Heeger, Peter S.
author_role author
author2 Harris, Cynthia
Abecassis, Michael
Anderson, Lisa
Bestard Matamoros, Oriol
Comai, Giorgia
Cravedi, Paolo
Cremaschi, Elena
Duty, J. Andrew
Florman, Sander
Friedewald, John
La Manna, Gaetano
Maggiore, Umberto
Moran, Thomas
Piotti, Giovanni
Purroy, Carolina
Jarque, Marta
Nair, Vinay
Shapiro, Ron
Reid-Adam, Jessica
Heeger, Peter S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rebuig (Biologia)
Quimiocines
Trasplantament renal
Interferometria
Graft rejection
Chemokines
Kidney transplantation
Interferometry
topic Rebuig (Biologia)
Quimiocines
Trasplantament renal
Interferometria
Graft rejection
Chemokines
Kidney transplantation
Interferometry
description Introduction: measuring the chemokine CXCL9 in urine by enzyme-linked immunosorbent assay (ELISA) can diagnose acute cellular rejection (ACR) noninvasively after kidney transplantation, but the required 12- to 24-hour turnaround time is not ideal for rapid, clinical decision-making. Methods: we developed a biolayer interferometry (BLI)−based assay to rapidly measure urinary CXCL9 in <1 hour. We validated this new assay versus standard ELISA in 86 urine samples from kidney transplantation recipients with various diagnoses. We then used BLI to analyze samples from 56 kidney transplantation recipients, including 46 subjects who experienced an acute rise in serum creatinine associated with biopsy-proven ACR (n = 22), subclinical rejection (n = 15), or no infiltrates (n = 9), and 10 stable kidney transplantation recipients with surveillance biopsies. To assess its usefulness in detecting adequacy of therapy we serially measured serum creatinine and urinary CXCL9 in 6 subjects after treatment for ACR, and correlated the results with histological diagnoses on follow-up biopsies. Results: BLI accurately and reproducibly detected urinary CXCL9 in <1 hour. BLI-based results showed that urinary CXCL9 was >200 pg/ml in subjects with ACR and ≤100 pg/ml in subjects with stable kidney function without cellular infiltrates. In samples obtained after treatment for ACR, BLI CXCL9 measurements detected biopsy-proven intragraft infiltrates despite treatment-induced reduction in serum creatinine. Discussion: together, our proof-of-principle results demonstrate that BLI-based urinary CXCL9 detection has potential as a point-of-care noninvasive biomarker to diagnose and guide therapy for ACR in kidney transplantation recipients.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/133090
url https://hdl.handle.net/2445/133090
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.ekir.2017.06.010
Kidney International Reports, 2017, vol. 2, num. 6, p. 1186-1193
https://doi.org/10.1016/j.ekir.2017.06.010
dc.rights.none.fl_str_mv cc-by-nc-nd (c) International Society of Nephrology, 2017
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) International Society of Nephrology, 2017
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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