Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation
Extracorporeal photopheresis (ECP) is an immunomodulatory therapy based on the infusion of autologous cellular products exposed to ultraviolet light (UV) in the presence of a photosensitizer. The study evaluates the ECP efficacy as induction therapy in a full-mismatch kidney transplant rat model. Da...
| Autores: | , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/224373 |
| Acceso en línea: | https://hdl.handle.net/2445/224373 |
| Access Level: | acceso abierto |
| Palabra clave: | Immunologia de la trasplantació Trasplantament renal Transplantation immunology Kidney transplantation |
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Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney TransplantationPiñeiro, Gastón JulioLazo Rodríguez, MartaVentura Abreu Aguiarà, PedroRamírez Bajo, María JoséBañón Maneus, ElisendaLozano Molero, MiguelCid Vidal, JoanHierro García, NataliaCucchiari, DavidRevuelta Vicente, IgnacioMontagud Marrahi, EnriquePalou Ribera, EduardBayés Genís, Beatriz EnriquetaCampistol Plana, Josep M.Diekmann, FritzRovira Juárez, JordiImmunologia de la trasplantacióTrasplantament renalTransplantation immunologyKidney transplantationExtracorporeal photopheresis (ECP) is an immunomodulatory therapy based on the infusion of autologous cellular products exposed to ultraviolet light (UV) in the presence of a photosensitizer. The study evaluates the ECP efficacy as induction therapy in a full-mismatch kidney transplant rat model. Dark Agouti to Lewis (DA-L) kidney transplant model has been established. ECP product was obtained from Lewis rat recipients after DA kidney graft transplantation (LewDA). Leukocytes of those LewDA rats were exposed to 8-methoxy psoralen, and illuminated with UV-A. The ECP doses assessed were 10 × 106 and 100 × 106 cells/time point. Lewis recipients received seven ECP infusions. DA-L model was characterized by the appearance of donor-specific antibodies (DSA) and kidney function deterioration from day three after kidney transplant. The dysfunction progressed rapidly until graft loss (6.1 ± 0.5 days). Tacrolimus at 0.25 mg/kg prolonged rat survival until 11.4 ± 0.7 days (p = 0.0004). In this context, the application of leukocytes from LewDA sensitized rats accelerated the rejection (8.7 ± 0.45, p = 0.0012), whereas ECP product at high dose extended kidney graft survival until 26.3 ± 7.3 days, reducing class I and II DSA in surviving rats. ECP treatment increases kidney graft survival in full-mismatch rat model of acute rejection and is a suitable immunomodulatory therapy to be explored in kidney transplantation.Copyright © 2023 Piñeiro, Lazo-Rodriguez, Ventura-Aguiar, Ramirez-Bajo, Banon-Maneus, Lozano, Cid, Hierro-Garcia, Cucchiari, Revuelta, Montagud-Marrahi, Palou, Bayés-Genís, Campistol, Diekmann and Rovira.Frontiers2025202520232025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/224373Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Reproducció del document publicat a: https://doi.org/10.3389/ti.2023.10840Transplant International, 2023, vol. 36, 10840https://doi.org/10.3389/ti.2023.10840cc-by (c) Piñeiro, Gastón Julio et al., 2023http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2243732026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation |
| title |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation |
| spellingShingle |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation Piñeiro, Gastón Julio Immunologia de la trasplantació Trasplantament renal Transplantation immunology Kidney transplantation |
| title_short |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation |
| title_full |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation |
| title_fullStr |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation |
| title_full_unstemmed |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation |
| title_sort |
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation |
| dc.creator.none.fl_str_mv |
Piñeiro, Gastón Julio Lazo Rodríguez, Marta Ventura Abreu Aguiarà, Pedro Ramírez Bajo, María José Bañón Maneus, Elisenda Lozano Molero, Miguel Cid Vidal, Joan Hierro García, Natalia Cucchiari, David Revuelta Vicente, Ignacio Montagud Marrahi, Enrique Palou Ribera, Eduard Bayés Genís, Beatriz Enriqueta Campistol Plana, Josep M. Diekmann, Fritz Rovira Juárez, Jordi |
| author |
Piñeiro, Gastón Julio |
| author_facet |
Piñeiro, Gastón Julio Lazo Rodríguez, Marta Ventura Abreu Aguiarà, Pedro Ramírez Bajo, María José Bañón Maneus, Elisenda Lozano Molero, Miguel Cid Vidal, Joan Hierro García, Natalia Cucchiari, David Revuelta Vicente, Ignacio Montagud Marrahi, Enrique Palou Ribera, Eduard Bayés Genís, Beatriz Enriqueta Campistol Plana, Josep M. Diekmann, Fritz Rovira Juárez, Jordi |
| author_role |
author |
| author2 |
Lazo Rodríguez, Marta Ventura Abreu Aguiarà, Pedro Ramírez Bajo, María José Bañón Maneus, Elisenda Lozano Molero, Miguel Cid Vidal, Joan Hierro García, Natalia Cucchiari, David Revuelta Vicente, Ignacio Montagud Marrahi, Enrique Palou Ribera, Eduard Bayés Genís, Beatriz Enriqueta Campistol Plana, Josep M. Diekmann, Fritz Rovira Juárez, Jordi |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Immunologia de la trasplantació Trasplantament renal Transplantation immunology Kidney transplantation |
| topic |
Immunologia de la trasplantació Trasplantament renal Transplantation immunology Kidney transplantation |
| description |
Extracorporeal photopheresis (ECP) is an immunomodulatory therapy based on the infusion of autologous cellular products exposed to ultraviolet light (UV) in the presence of a photosensitizer. The study evaluates the ECP efficacy as induction therapy in a full-mismatch kidney transplant rat model. Dark Agouti to Lewis (DA-L) kidney transplant model has been established. ECP product was obtained from Lewis rat recipients after DA kidney graft transplantation (LewDA). Leukocytes of those LewDA rats were exposed to 8-methoxy psoralen, and illuminated with UV-A. The ECP doses assessed were 10 × 106 and 100 × 106 cells/time point. Lewis recipients received seven ECP infusions. DA-L model was characterized by the appearance of donor-specific antibodies (DSA) and kidney function deterioration from day three after kidney transplant. The dysfunction progressed rapidly until graft loss (6.1 ± 0.5 days). Tacrolimus at 0.25 mg/kg prolonged rat survival until 11.4 ± 0.7 days (p = 0.0004). In this context, the application of leukocytes from LewDA sensitized rats accelerated the rejection (8.7 ± 0.45, p = 0.0012), whereas ECP product at high dose extended kidney graft survival until 26.3 ± 7.3 days, reducing class I and II DSA in surviving rats. ECP treatment increases kidney graft survival in full-mismatch rat model of acute rejection and is a suitable immunomodulatory therapy to be explored in kidney transplantation.Copyright © 2023 Piñeiro, Lazo-Rodriguez, Ventura-Aguiar, Ramirez-Bajo, Banon-Maneus, Lozano, Cid, Hierro-Garcia, Cucchiari, Revuelta, Montagud-Marrahi, Palou, Bayés-Genís, Campistol, Diekmann and Rovira. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2025 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/224373 |
| url |
https://hdl.handle.net/2445/224373 |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.3389/ti.2023.10840 Transplant International, 2023, vol. 36, 10840 https://doi.org/10.3389/ti.2023.10840 |
| dc.rights.none.fl_str_mv |
cc-by (c) Piñeiro, Gastón Julio et al., 2023 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Piñeiro, Gastón Julio et al., 2023 http://creativecommons.org/licenses/by/3.0/es/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
12 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers |
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Frontiers |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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