Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii

The α-glucosidase from the yeast Metschnikowia reukaufii, a producer of honey sugars, was immobilized on chitosan beads and biomimetic silica nanoparticles under mild and green conditions. Chitosan based derivatives showed apparent activities of about 40–70 U·g, while silica entrapped enzyme nanopar...

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Detalles Bibliográficos
Autores: Garcia-Gonzalez, Martín, Marmotéate, Egle, Cervantes, Fadia V., Plou Gasca, Francisco José, Betancor, Lorena, Fernandez-Lobato, María
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346672
Acceso en línea:http://hdl.handle.net/10261/346672
Access Level:acceso abierto
Palabra clave:Metschnikowia reukaufii
α-glucosidase
Isomelezitose
Chitosan beads
Biomimetic silica nanoparticles
Packed-bed reactor
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spelling Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufiiGarcia-Gonzalez, MartínMarmotéate, EgleCervantes, Fadia V.Plou Gasca, Francisco JoséBetancor, LorenaFernandez-Lobato, MaríaMetschnikowia reukaufiiα-glucosidaseIsomelezitoseChitosan beadsBiomimetic silica nanoparticlesPacked-bed reactorThe α-glucosidase from the yeast Metschnikowia reukaufii, a producer of honey sugars, was immobilized on chitosan beads and biomimetic silica nanoparticles under mild and green conditions. Chitosan based derivatives showed apparent activities of about 40–70 U·g, while silica entrapped enzyme nanoparticles contained 486 U·g. Compared to the soluble enzyme, the immobilized biocatalysts showed maximum hydrolytic activity at higher temperatures and broader pH interval, in addition to an excellent batch operational stability, maintaining 75–90% of its initial activity after ten subsequent cycles of 20 min reactions. Furthermore, chitosan beads and silica nanoparticles biocatalysts were packed into reactor columns and for the first time employed in the continuous overproduction of isomelezitose and trehalulose. Both systems operated continuously for 30 days with excellent isomelezitose yields of about 62 and 54% of the total sugar synthesised, respectively. The bioreactor based on chitosan beads had an average productivity of 0.5 kg L per day. This production was increased by almost 10 times (4.9 kg L per day) when a micro-reactor based on the biomimetic silica nanoparticles was used. Consequently, this work constitutes an interesting approach for the production of isomelezitose on an industrial scale, a new high-value functional sugar with interesting applications.The Spanish Ministries of Economy and Competitiveness (BIO2016-76601-C3-2/-1) and of Science and Innovation (PID2019-105838RB-C3-2/-1) and the Fundación Ramón Areces (XIX Call of Research Grants in Life and Materials Sciences) supported this work. We thank Mrs. Asunción Martín-Redondo for technical support, and Dr. Noa Míguez for her HPLC technical support. Martin Garcia-Gonzalez thanks the Spanish Ministry of Science, Innovation and Universities for the FPU Grant (FPU16/02925). We also thank Fundación Ramón Areces for an institutional grant to the Center of Molecular Biology Severo Ochoa.Peer reviewedMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Fundación Ramón ArecesMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420232024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/346672reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MECD//FPU16%2F02925info:eu-repo/grantAgreement/AEI//BIO2016-76601-C3-1-Rinfo:eu-repo/grantAgreement/AEI//BIO2016-76601-C3-2-Rinfo:eu-repo/grantAgreement/AEI//BIO2016-76601-C3-3-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105838RB-C31info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105838RB-C32info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105838RB-C33http://dx.doi.org/10.1016/j.bcab.2022.102590Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3466722026-05-22T06:33:51Z
dc.title.none.fl_str_mv Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
title Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
spellingShingle Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
Garcia-Gonzalez, Martín
Metschnikowia reukaufii
α-glucosidase
Isomelezitose
Chitosan beads
Biomimetic silica nanoparticles
Packed-bed reactor
title_short Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
title_full Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
title_fullStr Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
title_full_unstemmed Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
title_sort Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii
dc.creator.none.fl_str_mv Garcia-Gonzalez, Martín
Marmotéate, Egle
Cervantes, Fadia V.
Plou Gasca, Francisco José
Betancor, Lorena
Fernandez-Lobato, María
author Garcia-Gonzalez, Martín
author_facet Garcia-Gonzalez, Martín
Marmotéate, Egle
Cervantes, Fadia V.
Plou Gasca, Francisco José
Betancor, Lorena
Fernandez-Lobato, María
author_role author
author2 Marmotéate, Egle
Cervantes, Fadia V.
Plou Gasca, Francisco José
Betancor, Lorena
Fernandez-Lobato, María
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Fundación Ramón Areces
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Metschnikowia reukaufii
α-glucosidase
Isomelezitose
Chitosan beads
Biomimetic silica nanoparticles
Packed-bed reactor
topic Metschnikowia reukaufii
α-glucosidase
Isomelezitose
Chitosan beads
Biomimetic silica nanoparticles
Packed-bed reactor
description The α-glucosidase from the yeast Metschnikowia reukaufii, a producer of honey sugars, was immobilized on chitosan beads and biomimetic silica nanoparticles under mild and green conditions. Chitosan based derivatives showed apparent activities of about 40–70 U·g, while silica entrapped enzyme nanoparticles contained 486 U·g. Compared to the soluble enzyme, the immobilized biocatalysts showed maximum hydrolytic activity at higher temperatures and broader pH interval, in addition to an excellent batch operational stability, maintaining 75–90% of its initial activity after ten subsequent cycles of 20 min reactions. Furthermore, chitosan beads and silica nanoparticles biocatalysts were packed into reactor columns and for the first time employed in the continuous overproduction of isomelezitose and trehalulose. Both systems operated continuously for 30 days with excellent isomelezitose yields of about 62 and 54% of the total sugar synthesised, respectively. The bioreactor based on chitosan beads had an average productivity of 0.5 kg L per day. This production was increased by almost 10 times (4.9 kg L per day) when a micro-reactor based on the biomimetic silica nanoparticles was used. Consequently, this work constitutes an interesting approach for the production of isomelezitose on an industrial scale, a new high-value functional sugar with interesting applications.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346672
url http://hdl.handle.net/10261/346672
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MECD//FPU16%2F02925
info:eu-repo/grantAgreement/AEI//BIO2016-76601-C3-1-R
info:eu-repo/grantAgreement/AEI//BIO2016-76601-C3-2-R
info:eu-repo/grantAgreement/AEI//BIO2016-76601-C3-3-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105838RB-C31
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105838RB-C32
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105838RB-C33
http://dx.doi.org/10.1016/j.bcab.2022.102590
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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