Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis
Alzheimer's disease (AD) is a major public health challenge, with its onset occurring years before symptoms appear. Soluble amyloid-β (Aβ) oligomers are key species in AD pathogenesis and diagnosis, highlighting the need for early detection. This study investigates the intrinsic fluorescence of...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad de Santiago de Compostela (USC) |
| Repositorio: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| Idioma: | inglés |
| OAI Identifier: | oai:minerva.usc.gal:10347/43532 |
| Acceso en línea: | https://hdl.handle.net/10347/43532 |
| Access Level: | acceso abierto |
| Palabra clave: | Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| id |
ES_f04f4dfb3aa9e1aa6dc35ebbfaebca0b |
|---|---|
| oai_identifier_str |
oai:minerva.usc.gal:10347/43532 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosisNovo, MercedesIllodo Brea, SaraSeijas Cerceda, JesúsMoraes, Stella Hernández Faria DeRodríguez-Prieto, FlorAl-Soufi, WajihAlzheimer’s diseaseSoluble amyloid-b (Ab) oligomersPathogenesisdiagnosisAlzheimer's disease (AD) is a major public health challenge, with its onset occurring years before symptoms appear. Soluble amyloid-β (Aβ) oligomers are key species in AD pathogenesis and diagnosis, highlighting the need for early detection. This study investigates the intrinsic fluorescence of Aβ(1–40) (Aβ40) as a label-free approach to detecting early-stage oligomers. Aβ40 exhibits autofluorescence dominated by tyrosine emission, which undergoes a strong blue shift and quenching during oligomerization. Additionally, aggregation-induced emission (AIE) in the visible spectral region emerges, correlating with Aβ oligomer concentration and providing a means to detect and quantify oligomers. At the critical aggregation concentrations cac1 = 0.5 μM and cac2 = 19 μM, distinct aggregation behaviours are observed. By employing steady-state fluorescence spectroscopy, a widely accessible technique, these findings establish a direct link between early Aβ aggregation and intrinsic fluorescence changes. This approach eliminates the need for extrinsic probes, simplifying experimental procedures and reducing artefacts. Although further studies are required to develop a robust quantitative correlation for potential diagnostic applications, Aβ autofluorescence represents a promising strategy for investigating early aggregation processes in the context of ADRoyal society of chemistryUniversidade de Santiago de Compostela. Departamento de Química FísicaUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)20252025-07-2120252025-07-21journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/43532reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-120378RB-I00open accesshttp://purl.org/coar/access_right/c_abf2© the Owner Societies 2025. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licencehttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/435322026-06-15T12:47:27Z |
| dc.title.none.fl_str_mv |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| spellingShingle |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis Novo, Mercedes Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| title_short |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_full |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_fullStr |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_full_unstemmed |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_sort |
Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| dc.creator.none.fl_str_mv |
Novo, Mercedes Illodo Brea, Sara Seijas Cerceda, Jesús Moraes, Stella Hernández Faria De Rodríguez-Prieto, Flor Al-Soufi, Wajih |
| author |
Novo, Mercedes |
| author_facet |
Novo, Mercedes Illodo Brea, Sara Seijas Cerceda, Jesús Moraes, Stella Hernández Faria De Rodríguez-Prieto, Flor Al-Soufi, Wajih |
| author_role |
author |
| author2 |
Illodo Brea, Sara Seijas Cerceda, Jesús Moraes, Stella Hernández Faria De Rodríguez-Prieto, Flor Al-Soufi, Wajih |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade de Santiago de Compostela. Departamento de Química Física Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS) |
| dc.subject.none.fl_str_mv |
Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| topic |
Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| description |
Alzheimer's disease (AD) is a major public health challenge, with its onset occurring years before symptoms appear. Soluble amyloid-β (Aβ) oligomers are key species in AD pathogenesis and diagnosis, highlighting the need for early detection. This study investigates the intrinsic fluorescence of Aβ(1–40) (Aβ40) as a label-free approach to detecting early-stage oligomers. Aβ40 exhibits autofluorescence dominated by tyrosine emission, which undergoes a strong blue shift and quenching during oligomerization. Additionally, aggregation-induced emission (AIE) in the visible spectral region emerges, correlating with Aβ oligomer concentration and providing a means to detect and quantify oligomers. At the critical aggregation concentrations cac1 = 0.5 μM and cac2 = 19 μM, distinct aggregation behaviours are observed. By employing steady-state fluorescence spectroscopy, a widely accessible technique, these findings establish a direct link between early Aβ aggregation and intrinsic fluorescence changes. This approach eliminates the need for extrinsic probes, simplifying experimental procedures and reducing artefacts. Although further studies are required to develop a robust quantitative correlation for potential diagnostic applications, Aβ autofluorescence represents a promising strategy for investigating early aggregation processes in the context of AD |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025-07-21 2025 2025-07-21 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10347/43532 |
| url |
https://hdl.handle.net/10347/43532 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-120378RB-I00 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 http://creativecommons.org/licenses/by-nc/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Royal society of chemistry |
| publisher.none.fl_str_mv |
Royal society of chemistry |
| dc.source.none.fl_str_mv |
reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname:Universidad de Santiago de Compostela (USC) |
| instname_str |
Universidad de Santiago de Compostela (USC) |
| reponame_str |
Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| collection |
Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869423944744828928 |
| score |
15,81155 |