Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria

In cyanobacteria, DNA-binding proteins from starved cells (Dps) play an important role in the cellular response to oxidative and nutritional stresses. In this study, we have characterized the cell-type specificity and the promoter regions of two orthologous dps genes, Npun_R5799 in Nostoc punctiform...

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Autores: Li, Xin, Nenninger, Anja, Muro-Pastor, Alicia M., Stensjö, Karin
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/117751
Acceso en línea:http://hdl.handle.net/10261/117751
Access Level:acceso abierto
Palabra clave:Cyanobacteria
Nostoc
Anabaena
Heterocyst differentiation
Ferritin-19 like protein
ROS scavenging
DIF motif
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spelling Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteriaLi, XinNenninger, AnjaMuro-Pastor, Alicia M.Stensjö, KarinCyanobacteriaNostocAnabaenaHeterocyst differentiationFerritin-19 like proteinROS scavengingDIF motifIn cyanobacteria, DNA-binding proteins from starved cells (Dps) play an important role in the cellular response to oxidative and nutritional stresses. In this study, we have characterized the cell-type specificity and the promoter regions of two orthologous dps genes, Npun_R5799 in Nostoc punctiforme and alr3808 in Anabaena sp. PCC 7120. A transcriptional start site (TSS), identical in location to the previously identified proximal TSS of alr3808, was identified for Npun_R5799 under both combined nitrogen supplemented and N2-fixing growth conditions. However, only alr3808 was also transcribed from a second distal TSS. Sequence homologies suggest that the promoter region containing the distal TSS is not conserved upstream of orthologous genes among heterocyst-forming cyanobacteria. The analysis of promoter GFP-reporter strains showed a different role in governing cell-type specificity between the proximal and distal promoter of alr3808. We here confirmed the heterocyst specificity of the distal promoter of alr3808 and described a very early induction of its expression during proheterocyst differentiation. In contrast, the complete promoters of both genes were active in all cells. Even though Npun_R5799 and alr3808 are orthologs, the regulation of their respective expression differs, indicating distinctions in the function of these cyanobacterial Dps proteins depending on the strain and cell type.Peer reviewedOxford University PressConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/117751reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/femsle/fnv017.Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1177512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
title Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
spellingShingle Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
Li, Xin
Cyanobacteria
Nostoc
Anabaena
Heterocyst differentiation
Ferritin-19 like protein
ROS scavenging
DIF motif
title_short Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
title_full Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
title_fullStr Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
title_full_unstemmed Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
title_sort Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
dc.creator.none.fl_str_mv Li, Xin
Nenninger, Anja
Muro-Pastor, Alicia M.
Stensjö, Karin
author Li, Xin
author_facet Li, Xin
Nenninger, Anja
Muro-Pastor, Alicia M.
Stensjö, Karin
author_role author
author2 Nenninger, Anja
Muro-Pastor, Alicia M.
Stensjö, Karin
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cyanobacteria
Nostoc
Anabaena
Heterocyst differentiation
Ferritin-19 like protein
ROS scavenging
DIF motif
topic Cyanobacteria
Nostoc
Anabaena
Heterocyst differentiation
Ferritin-19 like protein
ROS scavenging
DIF motif
description In cyanobacteria, DNA-binding proteins from starved cells (Dps) play an important role in the cellular response to oxidative and nutritional stresses. In this study, we have characterized the cell-type specificity and the promoter regions of two orthologous dps genes, Npun_R5799 in Nostoc punctiforme and alr3808 in Anabaena sp. PCC 7120. A transcriptional start site (TSS), identical in location to the previously identified proximal TSS of alr3808, was identified for Npun_R5799 under both combined nitrogen supplemented and N2-fixing growth conditions. However, only alr3808 was also transcribed from a second distal TSS. Sequence homologies suggest that the promoter region containing the distal TSS is not conserved upstream of orthologous genes among heterocyst-forming cyanobacteria. The analysis of promoter GFP-reporter strains showed a different role in governing cell-type specificity between the proximal and distal promoter of alr3808. We here confirmed the heterocyst specificity of the distal promoter of alr3808 and described a very early induction of its expression during proheterocyst differentiation. In contrast, the complete promoters of both genes were active in all cells. Even though Npun_R5799 and alr3808 are orthologs, the regulation of their respective expression differs, indicating distinctions in the function of these cyanobacterial Dps proteins depending on the strain and cell type.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/117751
url http://hdl.handle.net/10261/117751
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/femsle/fnv017.
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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