High-Quality In Vivo Chemical Probes for Protein Kinases Disclosed in 2024

Protein kinases are highly relevant drug targets, yet a significant fraction of the human kinome remains underexplored. Highly potent and selective small-molecule inhibitors used as chemical probes are invaluable tools for enabling the validation and translation of new kinase targets. This review pr...

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Bibliographic Details
Authors: Massarico Serafim, Ricardo Augusto, Gehringer, Matthias
Format: article
Publication Date:2025
Country:España
Institution:Universitat Ramon Llull (URL)
Repository:DAU Arxiu Digital de la Universitat Ramon Llull
OAI Identifier:oai:dau.url.edu:20.500.14342/5518
Online Access:http://hdl.handle.net/20.500.14342/5518
https://doi.org/10.1021/acsptsci.5c00293
Access Level:Open access
Keyword:Chemical probes
Protein kinase inhibitors
Target validation
Drug discovery
Understudied kinome
Proteïnes quinases--Inhibidors
Descobriment de fàrmacs
577
Description
Summary:Protein kinases are highly relevant drug targets, yet a significant fraction of the human kinome remains underexplored. Highly potent and selective small-molecule inhibitors used as chemical probes are invaluable tools for enabling the validation and translation of new kinase targets. This review provides an overview and analysis of the high-quality in vivo chemical probes for protein kinases published and deposited at the Chemical Probes Portal in the year 2024. We discuss the design strategies, molecular mechanism of action, details of their use in vitro and in vivo, as well as their application in target (in)validation. We also highlight the importance of wisely selecting chemical probes and encourage best practices in using such tool compounds.