NanoFN10: A High-Contrast Turn-On Fluorescence Nanoprobe for Multiphoton Singlet Oxygen Imaging

An “off-on” fluorescent nanoprobe for near-infrared multiphoton imaging of singlet oxygen has been developed. The nanoprobe comprises a naphthoxazole fluorescent unit and a singlet-oxygen-sensitive furan derivative attached to the surface of mesoporous silica nanoparticles. In solution, the fluoresc...

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Bibliographic Details
Authors: Zanocco, Renzo, Bresolí Obach, Roger, Nájera, Francisco, Pérez de Inestrosa, Ezequiel, Zanocco, Antonio, Lemp, Else, Nonell, Santi
Format: article
Publication Date:2023
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/4662
Online Access:http://hdl.handle.net/20.500.14342/4662
https://doi.org/10.3390/s23104603
Access Level:Open access
Keyword:Singlet oxygen
Fluorescent nanoprobe
Multiphoton excitation
Oxigen
Fluorescència
Microscòpia--Tècnica
543
Description
Summary:An “off-on” fluorescent nanoprobe for near-infrared multiphoton imaging of singlet oxygen has been developed. The nanoprobe comprises a naphthoxazole fluorescent unit and a singlet-oxygen-sensitive furan derivative attached to the surface of mesoporous silica nanoparticles. In solution, the fluorescence of the nanoprobe increases upon reaction with singlet oxygen both under one- and multiphoton excitation, with fluorescence enhancements up to 180-fold. The nanoprobe can be readily internalized by macrophage cells and is capable of imaging intracellular singlet oxygen under multiphoton excitation.