High frequency and founder effect of the CYP3A4*20 loss-of-function allele in the Spanish population classifies CYP3A4 as a polymorphic enzyme.

Cytochrome P450 3A4 (CYP3A4) is a key drug-metabolizing enzyme. Loss-of-function variants have been reported as rare events, and the first demonstration of a CYP3A4 protein lacking functional activity is caused by CYP3A4*20 allele. Here we characterized the world distribution and origin of CYP3A4*20...

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Detalles Bibliográficos
Autores: Apellániz-Ruiz, M, Inglada-Pérez, L, Naranjo, M E G, Sánchez, L, Mancikova, V, Currás-Freixes, M, de Cubas, A A, Comino-Méndez, I, Triki, S, Rebai, A, Rasool, M, Moya, G, Grazina, M, Opocher, G, Cascón, A, Taboada-Echalar, P, Ingelman-Sundberg, M, Carracedo, A, Robledo Batanero, Mercedes, Llerena, A, Rodriguez Antona, Cristina
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17505
Acceso en línea:http://hdl.handle.net/20.500.12105/17505
Access Level:acceso abierto
Palabra clave:Alleles
Cytochrome P-450 CYP3A
Descripción
Sumario:Cytochrome P450 3A4 (CYP3A4) is a key drug-metabolizing enzyme. Loss-of-function variants have been reported as rare events, and the first demonstration of a CYP3A4 protein lacking functional activity is caused by CYP3A4*20 allele. Here we characterized the world distribution and origin of CYP3A4*20 mutation. CYP3A4*20 was determined in more than 4000 individuals representing different populations, and haplotype analysis was performed using CYP3A polymorphisms and microsatellite markers. CYP3A4*20 allele was present in 1.2% of the Spanish population (up to 3.8% in specific regions), and all CYP3A4*20 carriers had a common haplotype. This is compatible with a Spanish founder effect and classifies CYP3A4 as a polymorphic enzyme. This constitutes the first description of a CYP3A4 loss-of-function variant with high frequency in a population. CYP3A4*20 results together with the key role of CYP3A4 in drug metabolism support screening for rare CYP3A4 functional alleles among subjects with adverse drug events in certain populations.