Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker

The development of Cannabis sativa strains with high cannabidiol (CBD) and low tetrahydrocannabinol (THC) content is a growing field of research, both for medical and recreational use. However, the mechanisms behind clinical actions of cannabinoids are still under investigation, although there is gr...

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Autores: Noskova, Ekaterina, Fernández, Roberto, García, Javier, Ochoa, Eneko, Domínguez-Fernández, Celtia, Adell, Albert, Laso, Antonio, Andrés, María Fé, González-Coloma, Azucena, Astigarraga, Egoitz, Barreda-Gómez, Gabriel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/339445
Acceso en línea:http://hdl.handle.net/10261/339445
Access Level:acceso abierto
Palabra clave:Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
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spelling Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a BiomarkerNoskova, EkaterinaFernández, RobertoGarcía, JavierOchoa, EnekoDomínguez-Fernández, CeltiaAdell, AlbertLaso, AntonioAndrés, María FéGonzález-Coloma, AzucenaAstigarraga, EgoitzBarreda-Gómez, GabrielCannabinoidsCannabis sativa extractsTHCCBDCytochrome c oxidaseMitochondrial activityThe development of Cannabis sativa strains with high cannabidiol (CBD) and low tetrahydrocannabinol (THC) content is a growing field of research, both for medical and recreational use. However, the mechanisms behind clinical actions of cannabinoids are still under investigation, although there is growing evidence that mitochondria play an important role in many of them. Numerous studies have described that cannabinoids modulate mitochondrial activity both through activation of mitochondrial cannabinoid receptors and through direct action on other proteins such as mitochondrial complexes involved in cellular respiration. Thus, the aim of this study was to determine the actions of a panel of extracts, isolated from high-CBD varieties of Cannabis sativa, on the activity of the mitochondrial electron transport chain complex IV, cytochrome c oxidase (CCO), in order to select those with a safer profile. After demonstrating that Cannabis sativa strains could be identified by cannabinoids content, concentration–response curves were performed with a collection of extracts from strains with high-CBD and low-THC content using bovine CCO. The CCO rate was clearly modified by specific extracts of Cannabis sativa plants compared to others. Half maximal inhibitory concentrations (IC50) of extracts and the inhibitory effects evoked at 1 × 10−4 g/mL displayed a significant correlation with the THC. Therefore, the screening of extracts based on CCO activity provides a powerful and rapid methodology to identify those plants with higher mitochondrial toxicity or even mito-protective actions.This work has been financed by grant PID2019-106222RB-C31/SRA, the Spanish Government (Ayudas para contratos para la formación de doctores en empresas (Doctorados Industriales) 2020, DIN2020-011349), and Instituto de Salud Carlos III (Grant number PI19/00170).Multidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Instituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/339445reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106222RB-C31info:eu-repo/grantAgreement/AEI//DIN2020-011349The underlying dataset has been published as supplementary material of the article in the publisher platform at https://www.mdpi.com/article/10.3390/ijms24021315/s1.http://dx.doi.org/10.3390/ijms24021315Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3394452026-05-22T06:33:51Z
dc.title.none.fl_str_mv Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
title Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
spellingShingle Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
Noskova, Ekaterina
Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
title_short Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
title_full Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
title_fullStr Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
title_full_unstemmed Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
title_sort Screening System of Cannabis sativa Extracts Based on Their Mitochondrial Safety Profile Using Cytochrome c Oxidase Activity as a Biomarker
dc.creator.none.fl_str_mv Noskova, Ekaterina
Fernández, Roberto
García, Javier
Ochoa, Eneko
Domínguez-Fernández, Celtia
Adell, Albert
Laso, Antonio
Andrés, María Fé
González-Coloma, Azucena
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
author Noskova, Ekaterina
author_facet Noskova, Ekaterina
Fernández, Roberto
García, Javier
Ochoa, Eneko
Domínguez-Fernández, Celtia
Adell, Albert
Laso, Antonio
Andrés, María Fé
González-Coloma, Azucena
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
author_role author
author2 Fernández, Roberto
García, Javier
Ochoa, Eneko
Domínguez-Fernández, Celtia
Adell, Albert
Laso, Antonio
Andrés, María Fé
González-Coloma, Azucena
Astigarraga, Egoitz
Barreda-Gómez, Gabriel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
topic Cannabinoids
Cannabis sativa extracts
THC
CBD
Cytochrome c oxidase
Mitochondrial activity
description The development of Cannabis sativa strains with high cannabidiol (CBD) and low tetrahydrocannabinol (THC) content is a growing field of research, both for medical and recreational use. However, the mechanisms behind clinical actions of cannabinoids are still under investigation, although there is growing evidence that mitochondria play an important role in many of them. Numerous studies have described that cannabinoids modulate mitochondrial activity both through activation of mitochondrial cannabinoid receptors and through direct action on other proteins such as mitochondrial complexes involved in cellular respiration. Thus, the aim of this study was to determine the actions of a panel of extracts, isolated from high-CBD varieties of Cannabis sativa, on the activity of the mitochondrial electron transport chain complex IV, cytochrome c oxidase (CCO), in order to select those with a safer profile. After demonstrating that Cannabis sativa strains could be identified by cannabinoids content, concentration–response curves were performed with a collection of extracts from strains with high-CBD and low-THC content using bovine CCO. The CCO rate was clearly modified by specific extracts of Cannabis sativa plants compared to others. Half maximal inhibitory concentrations (IC50) of extracts and the inhibitory effects evoked at 1 × 10−4 g/mL displayed a significant correlation with the THC. Therefore, the screening of extracts based on CCO activity provides a powerful and rapid methodology to identify those plants with higher mitochondrial toxicity or even mito-protective actions.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/339445
url http://hdl.handle.net/10261/339445
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106222RB-C31
info:eu-repo/grantAgreement/AEI//DIN2020-011349
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://www.mdpi.com/article/10.3390/ijms24021315/s1.
http://dx.doi.org/10.3390/ijms24021315
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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