Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice

Reduced muscle activity leads to muscle atrophy and function loss in patients and animal models. Satellite cells (SCs) are postnatal muscle stem cells that play a pivotal role in skeletal muscle regeneration following injury. The regenerative potential, satellite cell numbers, and markers during rec...

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Detalhes bibliográficos
Autores: Guitart, Maria, Lloreta, Josep, 1958-, Mañas García, Laura, 1991-, Barreiro Portela, Esther
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Recursos:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/34873
Acesso em linha:http://hdl.handle.net/10230/34873
http://dx.doi.org/10.1002/jcp.26282
Access Level:acceso abierto
Palavra-chave:Músculs -- Fisiologia
Músculs -- Regeneració
Electron microscopy
Hindlimb immobilization
hindlimb recovery
Muscle progenitor cells and satellite cell counts
uscle regeneration markers
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spelling Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in miceGuitart, MariaLloreta, Josep, 1958-Mañas García, Laura, 1991-Barreiro Portela, EstherMúsculs -- FisiologiaMúsculs -- RegeneracióElectron microscopyHindlimb immobilizationhindlimb recoveryMuscle progenitor cells and satellite cell countsuscle regeneration markersReduced muscle activity leads to muscle atrophy and function loss in patients and animal models. Satellite cells (SCs) are postnatal muscle stem cells that play a pivotal role in skeletal muscle regeneration following injury. The regenerative potential, satellite cell numbers, and markers during recovery following immobilization of the hindlimb for 7 days were explored. In mice exposed to 7 days of hindlimb immobilization, in those exposed to recovery (7 days, splint removal), and in contralateral control muscles, muscle precursor cells were isolated from all hindlimb muscles (fluorescence-activated cell sorting, FACS) and SCs, and muscle regeneration were identified using immunofluorescence (gastrocnemius and soleus) and electron microscopy (EM, gastrocnemius). Expression of ki67, pax7, myoD, and myogenin was quantified (RT-PCR) from SC FACS yields. Body and grip strength were determined. Following 7 day hindlimb immobilization, a decline in SCs (FACS, immunofluorescence) was observed together with an upregulation of SC activation markers and signs of muscle regeneration including fusion to existing myofibers (EM). Recovery following hindlimb immobilization was characterized by a program of muscle regeneration events. Hindlimb immobilization induced a decline in SCs together with an upregulation of markers of SC activation, suggesting that fusion to existing myofibers takes place during unloading. Muscle recovery induced a significant rise in muscle precursor cells and regeneration events along with reduced SC activation expression markers and a concomitant rise in terminal muscle differentiation expression. These are novel findings of potential applicability for the treatment of disuse muscle atrophy, which is commonly associated with severe chronic and acute conditions.Wiley20182018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/34873http://dx.doi.org/10.1002/jcp.26282reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésJournal of Cellular Physiology. 2018 May;233(5):4360-72This is the peer reviewed version of the following article: Guitart M, Lloreta J, Mañas-Garcia L, Barreiro E. Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice. J Cell Physiol 2018 May; 233(5): 4360-4372, which has been published in final form at http://dx.doi.org/10.1002/jcp.26282. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/348732026-06-12T07:21:37Z
dc.title.none.fl_str_mv Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
title Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
spellingShingle Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
Guitart, Maria
Músculs -- Fisiologia
Músculs -- Regeneració
Electron microscopy
Hindlimb immobilization
hindlimb recovery
Muscle progenitor cells and satellite cell counts
uscle regeneration markers
title_short Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
title_full Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
title_fullStr Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
title_full_unstemmed Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
title_sort Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice
dc.creator.none.fl_str_mv Guitart, Maria
Lloreta, Josep, 1958-
Mañas García, Laura, 1991-
Barreiro Portela, Esther
author Guitart, Maria
author_facet Guitart, Maria
Lloreta, Josep, 1958-
Mañas García, Laura, 1991-
Barreiro Portela, Esther
author_role author
author2 Lloreta, Josep, 1958-
Mañas García, Laura, 1991-
Barreiro Portela, Esther
author2_role author
author
author
dc.subject.none.fl_str_mv Músculs -- Fisiologia
Músculs -- Regeneració
Electron microscopy
Hindlimb immobilization
hindlimb recovery
Muscle progenitor cells and satellite cell counts
uscle regeneration markers
topic Músculs -- Fisiologia
Músculs -- Regeneració
Electron microscopy
Hindlimb immobilization
hindlimb recovery
Muscle progenitor cells and satellite cell counts
uscle regeneration markers
description Reduced muscle activity leads to muscle atrophy and function loss in patients and animal models. Satellite cells (SCs) are postnatal muscle stem cells that play a pivotal role in skeletal muscle regeneration following injury. The regenerative potential, satellite cell numbers, and markers during recovery following immobilization of the hindlimb for 7 days were explored. In mice exposed to 7 days of hindlimb immobilization, in those exposed to recovery (7 days, splint removal), and in contralateral control muscles, muscle precursor cells were isolated from all hindlimb muscles (fluorescence-activated cell sorting, FACS) and SCs, and muscle regeneration were identified using immunofluorescence (gastrocnemius and soleus) and electron microscopy (EM, gastrocnemius). Expression of ki67, pax7, myoD, and myogenin was quantified (RT-PCR) from SC FACS yields. Body and grip strength were determined. Following 7 day hindlimb immobilization, a decline in SCs (FACS, immunofluorescence) was observed together with an upregulation of SC activation markers and signs of muscle regeneration including fusion to existing myofibers (EM). Recovery following hindlimb immobilization was characterized by a program of muscle regeneration events. Hindlimb immobilization induced a decline in SCs together with an upregulation of markers of SC activation, suggesting that fusion to existing myofibers takes place during unloading. Muscle recovery induced a significant rise in muscle precursor cells and regeneration events along with reduced SC activation expression markers and a concomitant rise in terminal muscle differentiation expression. These are novel findings of potential applicability for the treatment of disuse muscle atrophy, which is commonly associated with severe chronic and acute conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/34873
http://dx.doi.org/10.1002/jcp.26282
url http://hdl.handle.net/10230/34873
http://dx.doi.org/10.1002/jcp.26282
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Cellular Physiology. 2018 May;233(5):4360-72
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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