In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis

Monocytes and regulatory noncoding RNAs play a crucial role in the development of atherosclerosis (ATH). We have previously shown that miR-125b-5p was upregulated in aortic macrophages, and the aim of this paper was to further study the "in vivo" impact of miR-125b-5p in ATH progression. E...

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Autores: Mallén, A, Rotllan, N, Griñán, R, Varela, C, Bertolino, E, Paloschi, V, Maegdefessel, L, Escolà-Gil, JC, Aran, JM, Sbraga, F, Blasco-Lucas, A, Torras, J, Navarro, E, Hueso, M
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p21092
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=21092
Access Level:acceso abierto
Palabra clave:atherosclerosis
CCR7
macrophages
miR-125b-5p
miRNAs
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spelling In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosisMallén, ARotllan, NGriñán, RVarela, CBertolino, EPaloschi, VMaegdefessel, LEscolà-Gil, JCAran, JMSbraga, FBlasco-Lucas, ATorras, JNavarro, EHueso, MatherosclerosisCCR7macrophagesmiR-125b-5pmiRNAsMonocytes and regulatory noncoding RNAs play a crucial role in the development of atherosclerosis (ATH). We have previously shown that miR-125b-5p was upregulated in aortic macrophages, and the aim of this paper was to further study the "in vivo" impact of miR-125b-5p in ATH progression. Eight-weeks-old ApoE(-/-) mice, fed with a high-fat diet for 14 wk, were treated with a miR-125b-5p mimic, with its specific antagonist (antagomiR-125b), with a control scrambled sequence (control oligonucleotide SC) or with a control vehicle with phosphate-buffered saline (PBS) for 4 wk. Treatment with the miR-125b-5p mimic increased plaque sizes, macrophage infiltration, and NF-kappa B activation compared to PBS control, independently of cholesterol levels. In contrast, treatment with a specific antagomir produced opposite effects and increased the number of M2 macrophages. Finally, the miR-125b-5p mimic was found to reduce expression of the chemokine receptor CCR7 in the human monocyte cell line THP-1 cells, and the mouse macrophage-like cell line RAW264.7 cells, as well as in the aortas and livers of mice, whereas the antagomiR-125b increased CCR7 expression. Reduced CCR7 expression was also observed in the aorta of patients with coronary artery disease. miR-125b-5p mimic increased inflammation and ATH progression. Targeting miR-125b-5p with a specific antagomir reduced plaque size and macrophage infiltration and increased expression of the chemokine receptor CCR7. These results support a role for miR-125b-5p in the upregulation of CCR7 expression and monocyte trafficking, thus restricting vascular inflammation in ATH progression.AMER PHYSIOLOGICAL SOC2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=21092AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGYISSN: 03636143ISSNe: 15221563reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p210922026-06-14T12:41:47Z
dc.title.none.fl_str_mv In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
title In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
spellingShingle In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
Mallén, A
atherosclerosis
CCR7
macrophages
miR-125b-5p
miRNAs
title_short In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
title_full In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
title_fullStr In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
title_full_unstemmed In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
title_sort In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
dc.creator.none.fl_str_mv Mallén, A
Rotllan, N
Griñán, R
Varela, C
Bertolino, E
Paloschi, V
Maegdefessel, L
Escolà-Gil, JC
Aran, JM
Sbraga, F
Blasco-Lucas, A
Torras, J
Navarro, E
Hueso, M
author Mallén, A
author_facet Mallén, A
Rotllan, N
Griñán, R
Varela, C
Bertolino, E
Paloschi, V
Maegdefessel, L
Escolà-Gil, JC
Aran, JM
Sbraga, F
Blasco-Lucas, A
Torras, J
Navarro, E
Hueso, M
author_role author
author2 Rotllan, N
Griñán, R
Varela, C
Bertolino, E
Paloschi, V
Maegdefessel, L
Escolà-Gil, JC
Aran, JM
Sbraga, F
Blasco-Lucas, A
Torras, J
Navarro, E
Hueso, M
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv atherosclerosis
CCR7
macrophages
miR-125b-5p
miRNAs
topic atherosclerosis
CCR7
macrophages
miR-125b-5p
miRNAs
description Monocytes and regulatory noncoding RNAs play a crucial role in the development of atherosclerosis (ATH). We have previously shown that miR-125b-5p was upregulated in aortic macrophages, and the aim of this paper was to further study the "in vivo" impact of miR-125b-5p in ATH progression. Eight-weeks-old ApoE(-/-) mice, fed with a high-fat diet for 14 wk, were treated with a miR-125b-5p mimic, with its specific antagonist (antagomiR-125b), with a control scrambled sequence (control oligonucleotide SC) or with a control vehicle with phosphate-buffered saline (PBS) for 4 wk. Treatment with the miR-125b-5p mimic increased plaque sizes, macrophage infiltration, and NF-kappa B activation compared to PBS control, independently of cholesterol levels. In contrast, treatment with a specific antagomir produced opposite effects and increased the number of M2 macrophages. Finally, the miR-125b-5p mimic was found to reduce expression of the chemokine receptor CCR7 in the human monocyte cell line THP-1 cells, and the mouse macrophage-like cell line RAW264.7 cells, as well as in the aortas and livers of mice, whereas the antagomiR-125b increased CCR7 expression. Reduced CCR7 expression was also observed in the aorta of patients with coronary artery disease. miR-125b-5p mimic increased inflammation and ATH progression. Targeting miR-125b-5p with a specific antagomir reduced plaque size and macrophage infiltration and increased expression of the chemokine receptor CCR7. These results support a role for miR-125b-5p in the upregulation of CCR7 expression and monocyte trafficking, thus restricting vascular inflammation in ATH progression.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=21092
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=21092
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv AMER PHYSIOLOGICAL SOC
publisher.none.fl_str_mv AMER PHYSIOLOGICAL SOC
dc.source.none.fl_str_mv AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
ISSN: 03636143
ISSNe: 15221563
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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