Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer

[EN] Although assisted reproduction technologies (ARTs) are recognised as safe, and most of the offspring seem apparently healthy, there is clear evidence that ARTs are associated with changes in the embryo's developmental trajectory, which incur physiological consequences during the prenat...

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Autores: Garcia-Dominguez, X, Diretto, Gianfranco, Frusciante, Sarah, Vicente Antón, José Salvador|||0000-0003-0481-3802, Marco-Jiménez, Francisco|||0000-0002-5226-4118
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/169423
Acceso en línea:https://riunet.upv.es/handle/10251/169423
Access Level:acceso abierto
Palabra clave:Embryo manipulation
Cryopreservation
Stress
Metabolism
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
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spelling Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified TransferGarcia-Dominguez, XDiretto, GianfrancoFrusciante, SarahVicente Antón, José Salvador|||0000-0003-0481-3802Marco-Jiménez, Francisco|||0000-0002-5226-4118Embryo manipulationCryopreservationStressMetabolismDevelopmental plasticityDevelopmental programmingBIOLOGIA ANIMALPRODUCCION ANIMAL[EN] Although assisted reproduction technologies (ARTs) are recognised as safe, and most of the offspring seem apparently healthy, there is clear evidence that ARTs are associated with changes in the embryo's developmental trajectory, which incur physiological consequences during the prenatal and postnatal stages of life. The present study aimed to address the influence of early (day-3 embryos) embryo transfer and cryopreservation on embryo survival, size, and metabolome at the preimplantation stage (day-6 embryos). To this end, fresh-transferred (FT) and vitrified-transferred (VT) embryos were compared using naturally-conceived (NC) embryos as a control reference. The results show that as in vitro manipulation was increased (NC < FT < VT), both embryo survival rate (0.91 +/- 0.02, 0.78 +/- 0.05 and 0.63 +/- 0.05, for NC, FT, and VT groups, respectively) and embryo size (3.21 +/- 0.49 mm, 2.15 +/- 0.51 mm, 1.76 +/- 0.46 mm of diameter for NC, FT, and VT groups, respectively) were significantly decreased. Moreover, an unbiased metabolomics analysis showed overall down-accumulation in 40 metabolites among the three experimental groups, with embryo transfer and embryo cryopreservation procedures both exerting a cumulative effect. In this regard, targeted metabolomics findings revealed a significant reduction in some metabolites involved in metabolic pathways, such as the Krebs cycle, amino acids, unsaturated fatty acids, and arachidonic acid metabolisms. Altogether, these findings highlight a synergistic effect between the embryo transfer and vitrification procedures in preimplantation embryos. However, the ex vivo manipulation during embryo transfer seemed to be the major trigger of the embryonic changes, as the deviations added by the vitrification process were relatively smaller.This research was funded by Conselleria d'Educacio, Investigacio, Cultura i Esport, Spain, grant number AICO/2019/272. Ximo Garcia-Dominguez was supported by a research grant from the Ministry of Economy, Industry and Competitiveness of Spain (BES-2015-072429).MDPI AGDepartamento de Ciencia AnimalInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalGeneralitat ValencianaMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20202020-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/169423reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BES-2015-072429 BES-2015-072429Generalitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2019%2F272open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1694232026-06-13T07:49:27Z
dc.title.none.fl_str_mv Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
title Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
spellingShingle Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
Garcia-Dominguez, X
Embryo manipulation
Cryopreservation
Stress
Metabolism
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
title_short Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
title_full Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
title_fullStr Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
title_full_unstemmed Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
title_sort Metabolomic Analysis Reveals Changes in Preimplantation Embryos Following Fresh or Vitrified Transfer
dc.creator.none.fl_str_mv Garcia-Dominguez, X
Diretto, Gianfranco
Frusciante, Sarah
Vicente Antón, José Salvador|||0000-0003-0481-3802
Marco-Jiménez, Francisco|||0000-0002-5226-4118
author Garcia-Dominguez, X
author_facet Garcia-Dominguez, X
Diretto, Gianfranco
Frusciante, Sarah
Vicente Antón, José Salvador|||0000-0003-0481-3802
Marco-Jiménez, Francisco|||0000-0002-5226-4118
author_role author
author2 Diretto, Gianfranco
Frusciante, Sarah
Vicente Antón, José Salvador|||0000-0003-0481-3802
Marco-Jiménez, Francisco|||0000-0002-5226-4118
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Ciencia Animal
Instituto Universitario de Ciencia y Tecnología Animal
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Generalitat Valenciana
Ministerio de Economía y Competitividad
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Embryo manipulation
Cryopreservation
Stress
Metabolism
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
topic Embryo manipulation
Cryopreservation
Stress
Metabolism
Developmental plasticity
Developmental programming
BIOLOGIA ANIMAL
PRODUCCION ANIMAL
description [EN] Although assisted reproduction technologies (ARTs) are recognised as safe, and most of the offspring seem apparently healthy, there is clear evidence that ARTs are associated with changes in the embryo's developmental trajectory, which incur physiological consequences during the prenatal and postnatal stages of life. The present study aimed to address the influence of early (day-3 embryos) embryo transfer and cryopreservation on embryo survival, size, and metabolome at the preimplantation stage (day-6 embryos). To this end, fresh-transferred (FT) and vitrified-transferred (VT) embryos were compared using naturally-conceived (NC) embryos as a control reference. The results show that as in vitro manipulation was increased (NC < FT < VT), both embryo survival rate (0.91 +/- 0.02, 0.78 +/- 0.05 and 0.63 +/- 0.05, for NC, FT, and VT groups, respectively) and embryo size (3.21 +/- 0.49 mm, 2.15 +/- 0.51 mm, 1.76 +/- 0.46 mm of diameter for NC, FT, and VT groups, respectively) were significantly decreased. Moreover, an unbiased metabolomics analysis showed overall down-accumulation in 40 metabolites among the three experimental groups, with embryo transfer and embryo cryopreservation procedures both exerting a cumulative effect. In this regard, targeted metabolomics findings revealed a significant reduction in some metabolites involved in metabolic pathways, such as the Krebs cycle, amino acids, unsaturated fatty acids, and arachidonic acid metabolisms. Altogether, these findings highlight a synergistic effect between the embryo transfer and vitrification procedures in preimplantation embryos. However, the ex vivo manipulation during embryo transfer seemed to be the major trigger of the embryonic changes, as the deviations added by the vitrification process were relatively smaller.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/169423
url https://riunet.upv.es/handle/10251/169423
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BES-2015-072429 BES-2015-072429
Generalitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2019%2F272
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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