In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment

Gold nanostars (AuNSTs) are biocompatible, have large surface areas, and are characterized by high near-infrared extinction, making them ideal for integration with technologies targeting biological applications. We have developed a robust and simple microfluidic method for the direct growth of aniso...

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Autores: Vinnacombe-Willson, Gail A., Chiang, Naihao, Scarabelli, Leonardo|||0000-0002-6830-5893, Hu, Yuan, Heidenreich, Liv K., Li, Xi, Gong, Yao, Inouye, Derek T., Fisher, Timothy S., Weiss, Paul S., Jonas, Steven J.
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/33641
Acceso en línea:https://hdl.handle.net/10902/33641
Access Level:acceso abierto
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
title In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
spellingShingle In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
Vinnacombe-Willson, Gail A.
title_short In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
title_full In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
title_fullStr In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
title_full_unstemmed In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
title_sort In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachment
dc.creator.none.fl_str_mv Vinnacombe-Willson, Gail A.
Chiang, Naihao
Scarabelli, Leonardo|||0000-0002-6830-5893
Hu, Yuan
Heidenreich, Liv K.
Li, Xi
Gong, Yao
Inouye, Derek T.
Fisher, Timothy S.
Weiss, Paul S.
Jonas, Steven J.
author Vinnacombe-Willson, Gail A.
author_facet Vinnacombe-Willson, Gail A.
Chiang, Naihao
Scarabelli, Leonardo|||0000-0002-6830-5893
Hu, Yuan
Heidenreich, Liv K.
Li, Xi
Gong, Yao
Inouye, Derek T.
Fisher, Timothy S.
Weiss, Paul S.
Jonas, Steven J.
author_role author
author2 Chiang, Naihao
Scarabelli, Leonardo|||0000-0002-6830-5893
Hu, Yuan
Heidenreich, Liv K.
Li, Xi
Gong, Yao
Inouye, Derek T.
Fisher, Timothy S.
Weiss, Paul S.
Jonas, Steven J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
description Gold nanostars (AuNSTs) are biocompatible, have large surface areas, and are characterized by high near-infrared extinction, making them ideal for integration with technologies targeting biological applications. We have developed a robust and simple microfluidic method for the direct growth of anisotropic AuNSTs on oxide substrates including indium tin oxide and glass. The synthesis was optimized to yield AuNSTs with high anisotropy, branching, uniformity, and density in batch and microfluidic systems for optimal light-to-heat conversion upon laser irradiation. Surface-enhanced Raman scattering spectra and mesoscale temperature measurements were combined with spatially correlated scanning electron microscopy to monitor nanostar and ligand stability and microbubble formation at different laser fluences. The capability of the platform for generating controlled localized heating was used to explore hyperthermia-assisted detachment of adherent glioblastoma cells (U87-GFP) grafted to the capillary walls. Both flow and laser fluence can be tuned to induce different biological responses, such as ablation, cell deformation, release of intracellular components, and the removal of intact cells. Ultimately, this platform has potential applications in biological and chemical sensing, hyperthermia-mediated drug delivery, and microfluidic soft-release of grafted cells with single-cell specificity.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-11-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10902/33641
url https://hdl.handle.net/10902/33641
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv ACS Central Science, 2020, 6(11), 2105-2116
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423907937714176
spelling In situ shape control of thermoplasmonic gold nanostars on oxide substrates for hyperthermia-mediated cell detachmentVinnacombe-Willson, Gail A.Chiang, NaihaoScarabelli, Leonardo|||0000-0002-6830-5893Hu, YuanHeidenreich, Liv K.Li, XiGong, YaoInouye, Derek T.Fisher, Timothy S.Weiss, Paul S.Jonas, Steven J.Gold nanostars (AuNSTs) are biocompatible, have large surface areas, and are characterized by high near-infrared extinction, making them ideal for integration with technologies targeting biological applications. We have developed a robust and simple microfluidic method for the direct growth of anisotropic AuNSTs on oxide substrates including indium tin oxide and glass. The synthesis was optimized to yield AuNSTs with high anisotropy, branching, uniformity, and density in batch and microfluidic systems for optimal light-to-heat conversion upon laser irradiation. Surface-enhanced Raman scattering spectra and mesoscale temperature measurements were combined with spatially correlated scanning electron microscopy to monitor nanostar and ligand stability and microbubble formation at different laser fluences. The capability of the platform for generating controlled localized heating was used to explore hyperthermia-assisted detachment of adherent glioblastoma cells (U87-GFP) grafted to the capillary walls. Both flow and laser fluence can be tuned to induce different biological responses, such as ablation, cell deformation, release of intracellular components, and the removal of intact cells. Ultimately, this platform has potential applications in biological and chemical sensing, hyperthermia-mediated drug delivery, and microfluidic soft-release of grafted cells with single-cell specificity.The authors thank Prof. Vincent Tung (KAUST) and Prof. H. R. Tseng (UCLA) for helpful comments and discussion, as well as Prof. Bruce Dunn (UCLA) and the Broad Center of Regenerative Medicine & Stem Cell Research for providing access to the instrumentation for the Raman measurements. Facility support was provided by the UCLA Clinical and Translational Science Institute (CTSI) Core Voucher Program supported through Grant Number UL1TR001881. L.S. thanks the American-Italian Cancer Foundation for fellowship support. N.C. thanks the NIH NIBIB for the Pathway to Independence Award (K99EB028325). Y.G. thanks the UCLA Department of Chemistry & Biochemistry for funding through the SG Fellowship. X.L. thanks the UCLA Cross-Disciplinary Scholars in Science & Technology program. S.J.J. is supported by the NIH Common Fund through a NIH Director’s Early Independence Award cofunded by the National Institute of Dental and Craniofacial Research and Office of the Director, NIH Grant DP5OD028181. S.J.J. also acknowledges Young Investigator Award funds from the Alex’s Lemonade Stand Foundation for Childhood Cancer Research, the Hyundai Hope on Wheels Foundation for Pediatric Cancer Research, and the Tower Cancer Research Foundation. Confocal laser scanning microscopy was performed at the Advanced Light Microscopy/Spectroscopy Laboratory and the Leica Microsystems Center of Excellence at the California NanoSystems Institute (CNSI) at UCLA with funding support from NIH Shared Instrumentation Grant S10OD025017 and NSF Major Research Instrumentation Grant CHE-0722519. Special thanks to Dr. Laurent Bentolila, Dr. Michael Lake, and Dr. Matthew Schibler for their helpful discussions and instrumentation support critical to the completion of this work. The authors acknowledge the use of instruments at the Electron Imaging Center for NanoMachines supported by NIH (1S10RR23057) and CNSI at UCLA. We dedicate this paper to Prof. Laura Kiessling as part of a special collection, on the occasion of her birthday.American Chemical SocietyUniversidad de Cantabria20202020-11-25journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttps://hdl.handle.net/10902/33641ACS Central Science, 2020, 6(11), 2105-2116reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/336412026-06-02T12:39:31Z
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