Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous

© 2020 Martinez-Moya et al.

Detalles Bibliográficos
Autores: Martinez-Moya, Pilar, Campusano, Sebastián, Cordova, Pamela, Paradela, Alberto, Sepulveda, Dionisia, Alcaíno, Jennifer, Baeza, Marcelo, Cifuentes, Víctor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/228831
Acceso en línea:http://hdl.handle.net/10261/228831
Access Level:acceso abierto
Palabra clave:iTRAQ
RNA-seq
Mig1
Cyc8
Tup1
Glucose
Maltose
Succinate
Catabolic repression
X. dendrorhous
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repository_id_str
spelling Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhousMartinez-Moya, PilarCampusano, SebastiánCordova, PamelaParadela, AlbertoSepulveda, DionisiaAlcaíno, JenniferBaeza, MarceloCifuentes, VíctoriTRAQRNA-seqMig1Cyc8Tup1GlucoseMaltoseSuccinateCatabolic repressionX. dendrorhous© 2020 Martinez-Moya et al.Xanthophyllomyces dendrorhous is a carotenogenic yeast with a singular metabolic capacity to produce astaxanthin, a valuable antioxidant pigment. This yeast can assimilate several carbon sources and sustain fermentation even under aerobic conditions. Since astaxanthin biosynthesis is affected by the carbon source, the study of carotenogenesis regulatory mechanisms is key for improving astaxanthin yield in X. dendrorhous This study aimed to elucidate the regulation of the metabolism of different carbon sources and the phenomenon of catabolic repression in this yeast. To this end, protein and transcript levels were quantified by iTRAQ (isobaric tags for relative and absolute quantification) and transcriptomic sequencing (RNA-seq) in the wild-type strain under conditions of glucose, maltose, or succinate treatment and in the mutant strains for genes MIG1, CYC8, and TUP1 under conditions of glucose treatment. Alternative carbon sources such as maltose and succinate affected the relative abundances of 14% of the wild-type proteins, which were mainly grouped into the carbohydrate metabolism category, with the glycolysis/gluconeogenesis and citrate cycle pathways being the most highly represented pathways. Each mutant strain showed significant proteomic profile changes, affecting approximately 2% of the total proteins identified, compared to the wild-type strain under glucose treatment conditions. Similarly to the results seen with the alternative carbon sources, the changes in the mutant strains mainly affected carbohydrate metabolism, with glycolysis/gluconeogenesis and the pentose phosphate and citrate cycle pathways being the most highly represented pathways. Our results showed convergence between carbon assimilation and catabolic repression in the strains studied. Interestingly, indications of cooperative, opposing, and overlapping processes during catabolic regulation were found. We also identified target proteins of the regulatory processes, reinforcing the likelihood of catabolic repression at the posttranscriptional level.IMPORTANCE The conditions affecting catabolic regulation in X. dendrorhous are complex and suggest the presence of an alternative mechanism of regulation. The repressors Mig1, Cyc8, and Tup1 are essential elements for the regulation of the use of glucose and other carbon sources. All play different roles but, depending on the growth conditions, can work in convergent, synergistic, and complementary ways to use carbon sources and to regulate other targets for yeast metabolism. Our results reinforced the belief that further studies in X. dendrorhous are needed to clarify a specific regulatory mechanism at the domain level of the repressors as well as its relationship with those of other metabolic repressors, i.e., the stress response, to elucidate carotenogenic regulation at the transcriptomic and proteomic levels in this yeast.This work was supported by FONDECYT 1180520, Postdoctoral fellowship Internationalization project grant UCH 1566 to P.M.-M., and by a graduate scholarship (PEEI) to S.C.American Society for MicrobiologyFondo Nacional de Desarrollo Científico y Tecnológico (Chile)Universidad de ChileConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/228831reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1128/mSphere.00065-20Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2288312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
title Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
spellingShingle Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
Martinez-Moya, Pilar
iTRAQ
RNA-seq
Mig1
Cyc8
Tup1
Glucose
Maltose
Succinate
Catabolic repression
X. dendrorhous
title_short Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
title_full Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
title_fullStr Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
title_full_unstemmed Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
title_sort Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous
dc.creator.none.fl_str_mv Martinez-Moya, Pilar
Campusano, Sebastián
Cordova, Pamela
Paradela, Alberto
Sepulveda, Dionisia
Alcaíno, Jennifer
Baeza, Marcelo
Cifuentes, Víctor
author Martinez-Moya, Pilar
author_facet Martinez-Moya, Pilar
Campusano, Sebastián
Cordova, Pamela
Paradela, Alberto
Sepulveda, Dionisia
Alcaíno, Jennifer
Baeza, Marcelo
Cifuentes, Víctor
author_role author
author2 Campusano, Sebastián
Cordova, Pamela
Paradela, Alberto
Sepulveda, Dionisia
Alcaíno, Jennifer
Baeza, Marcelo
Cifuentes, Víctor
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Universidad de Chile
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv iTRAQ
RNA-seq
Mig1
Cyc8
Tup1
Glucose
Maltose
Succinate
Catabolic repression
X. dendrorhous
topic iTRAQ
RNA-seq
Mig1
Cyc8
Tup1
Glucose
Maltose
Succinate
Catabolic repression
X. dendrorhous
description © 2020 Martinez-Moya et al.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/228831
url http://hdl.handle.net/10261/228831
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1128/mSphere.00065-20
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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