Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients

Diabetic patients present increased volume and functional alterations in epicardial adipose tissue (EAT). We aimed to analyze EAT from type 2 diabetic patients and the inflammatory and cytotoxic effects induced on cardiomyocytes. Furthermore, we analyzed the cardioprotective role of apolipoprotein J...

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Autores: Puig Grifol, Núria|||0000-0002-4699-3162, Rives Jimenez, Jose|||0009-0001-5725-9270, Gil-Millan, Pedro, Miñambres, Inka|||0000-0002-0899-3938, Ginel, Antonino|||0000-0002-8820-7545, Tauron, Manel|||0000-0002-5864-519X, Bonaterra-Pastra, Anna|||0000-0002-1480-622X, Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091, Pérez, Antonio|||0000-0001-5528-1143, Sánchez Quesada, José Luis|||0000-0003-0224-591X, Benitez, Sonia|||0000-0003-3565-0743
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:306970
Acceso en línea:https://ddd.uab.cat/record/306970
https://dx.doi.org/urn:doi:10.1016/j.biopha.2024.116779
Access Level:acceso abierto
Palabra clave:Apolipoprotein J
Apoptosis
Ceramides
Coronary artery disease
Epicardial adipose tissue
Fatty acids
Inflammation
Type 2 diabetes
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spelling Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patientsPuig Grifol, Núria|||0000-0002-4699-3162Rives Jimenez, Jose|||0009-0001-5725-9270Gil-Millan, PedroMiñambres, Inka|||0000-0002-0899-3938Ginel, Antonino|||0000-0002-8820-7545Tauron, Manel|||0000-0002-5864-519XBonaterra-Pastra, Anna|||0000-0002-1480-622XHernandez Guillamon, Maria Mar|||0000-0001-8844-0091Pérez, Antonio|||0000-0001-5528-1143Sánchez Quesada, José Luis|||0000-0003-0224-591XBenitez, Sonia|||0000-0003-3565-0743Apolipoprotein JApoptosisCeramidesCoronary artery diseaseEpicardial adipose tissueFatty acidsInflammationType 2 diabetesDiabetic patients present increased volume and functional alterations in epicardial adipose tissue (EAT). We aimed to analyze EAT from type 2 diabetic patients and the inflammatory and cytotoxic effects induced on cardiomyocytes. Furthermore, we analyzed the cardioprotective role of apolipoprotein J (apoJ). EAT explants were obtained from nondiabetic patients (ND), diabetic patients without coronary disease (DM), and DM patients with coronary disease (DM-C) after heart surgery. Morphological characteristics and gene expression were evaluated. Explants were cultured for 24 h and the content of nonesterified fatty acids (NEFA) and sphingolipid species in secretomes was evaluated by lipidomic analysis. Afterwards, secretomes were added to AC16 human cardiomyocytes for 24 h in the presence or absence of cardioprotective molecules (apoJ and HDL). Cytokine release and apoptosis/necrosis were assessed by ELISA and flow cytometry. The EAT from the diabetic samples showed altered expression of genes related to lipid accumulation, insulin resistance, and inflammation. The secretomes from the DM samples presented an increased ratio of pro/antiatherogenic ceramide (Cer) species, while those from DM-C contained the highest concentration of saturated NEFA. DM and DM-C secretomes promoted inflammation and cytotoxicity on AC16 cardiomyocytes. Exogenous Cer16:0, Cer24:1, and palmitic acid reproduced deleterious effects in AC16 cells. These effects were attenuated by exogenous apoJ. Diabetic secretomes promoted inflammation and cytotoxicity in cardiomyocytes. This effect was exacerbated in the secretomes of the DM-C samples. The increased content of specific NEFA and ceramide species seems to play a key role in inducing such deleterious effects, which are attenuated by apoJ.Universitat Autònoma de Barcelona 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/306970https://dx.doi.org/urn:doi:10.1016/j.biopha.2024.116779reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI16/00471Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI20/00334Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1149open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3069702026-06-06T12:50:31Z
dc.title.none.fl_str_mv Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
title Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
spellingShingle Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
Puig Grifol, Núria|||0000-0002-4699-3162
Apolipoprotein J
Apoptosis
Ceramides
Coronary artery disease
Epicardial adipose tissue
Fatty acids
Inflammation
Type 2 diabetes
title_short Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
title_full Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
title_fullStr Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
title_full_unstemmed Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
title_sort Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
dc.creator.none.fl_str_mv Puig Grifol, Núria|||0000-0002-4699-3162
Rives Jimenez, Jose|||0009-0001-5725-9270
Gil-Millan, Pedro
Miñambres, Inka|||0000-0002-0899-3938
Ginel, Antonino|||0000-0002-8820-7545
Tauron, Manel|||0000-0002-5864-519X
Bonaterra-Pastra, Anna|||0000-0002-1480-622X
Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091
Pérez, Antonio|||0000-0001-5528-1143
Sánchez Quesada, José Luis|||0000-0003-0224-591X
Benitez, Sonia|||0000-0003-3565-0743
author Puig Grifol, Núria|||0000-0002-4699-3162
author_facet Puig Grifol, Núria|||0000-0002-4699-3162
Rives Jimenez, Jose|||0009-0001-5725-9270
Gil-Millan, Pedro
Miñambres, Inka|||0000-0002-0899-3938
Ginel, Antonino|||0000-0002-8820-7545
Tauron, Manel|||0000-0002-5864-519X
Bonaterra-Pastra, Anna|||0000-0002-1480-622X
Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091
Pérez, Antonio|||0000-0001-5528-1143
Sánchez Quesada, José Luis|||0000-0003-0224-591X
Benitez, Sonia|||0000-0003-3565-0743
author_role author
author2 Rives Jimenez, Jose|||0009-0001-5725-9270
Gil-Millan, Pedro
Miñambres, Inka|||0000-0002-0899-3938
Ginel, Antonino|||0000-0002-8820-7545
Tauron, Manel|||0000-0002-5864-519X
Bonaterra-Pastra, Anna|||0000-0002-1480-622X
Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091
Pérez, Antonio|||0000-0001-5528-1143
Sánchez Quesada, José Luis|||0000-0003-0224-591X
Benitez, Sonia|||0000-0003-3565-0743
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Apolipoprotein J
Apoptosis
Ceramides
Coronary artery disease
Epicardial adipose tissue
Fatty acids
Inflammation
Type 2 diabetes
topic Apolipoprotein J
Apoptosis
Ceramides
Coronary artery disease
Epicardial adipose tissue
Fatty acids
Inflammation
Type 2 diabetes
description Diabetic patients present increased volume and functional alterations in epicardial adipose tissue (EAT). We aimed to analyze EAT from type 2 diabetic patients and the inflammatory and cytotoxic effects induced on cardiomyocytes. Furthermore, we analyzed the cardioprotective role of apolipoprotein J (apoJ). EAT explants were obtained from nondiabetic patients (ND), diabetic patients without coronary disease (DM), and DM patients with coronary disease (DM-C) after heart surgery. Morphological characteristics and gene expression were evaluated. Explants were cultured for 24 h and the content of nonesterified fatty acids (NEFA) and sphingolipid species in secretomes was evaluated by lipidomic analysis. Afterwards, secretomes were added to AC16 human cardiomyocytes for 24 h in the presence or absence of cardioprotective molecules (apoJ and HDL). Cytokine release and apoptosis/necrosis were assessed by ELISA and flow cytometry. The EAT from the diabetic samples showed altered expression of genes related to lipid accumulation, insulin resistance, and inflammation. The secretomes from the DM samples presented an increased ratio of pro/antiatherogenic ceramide (Cer) species, while those from DM-C contained the highest concentration of saturated NEFA. DM and DM-C secretomes promoted inflammation and cytotoxicity on AC16 cardiomyocytes. Exogenous Cer16:0, Cer24:1, and palmitic acid reproduced deleterious effects in AC16 cells. These effects were attenuated by exogenous apoJ. Diabetic secretomes promoted inflammation and cytotoxicity in cardiomyocytes. This effect was exacerbated in the secretomes of the DM-C samples. The increased content of specific NEFA and ceramide species seems to play a key role in inducing such deleterious effects, which are attenuated by apoJ.
publishDate 2024
dc.date.none.fl_str_mv 2
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/306970
https://dx.doi.org/urn:doi:10.1016/j.biopha.2024.116779
url https://ddd.uab.cat/record/306970
https://dx.doi.org/urn:doi:10.1016/j.biopha.2024.116779
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI16/00471
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI20/00334
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1149
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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