Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.

Despite advances in bone graft design and surgical techniques, bacterial infection remains a major cause of graft failure, exacerbated by the global rise in antimicrobial resistance. This has intensified the pursuit of antibiotic-free strategies to prevent bacterial colonization. Among these, antiba...

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Detalles Bibliográficos
Autores: Arca-Garcia C, Godoy-Gallardo M, Ginebra MP
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p29717
Acceso en línea:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29717
Access Level:acceso abierto
Palabra clave:Antibacterial nanotopography
Bone regeneration
Calcium phosphate
Fluoride
Hydroxyapatite
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spelling Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.Arca-Garcia CGodoy-Gallardo MGinebra MPAntibacterial nanotopographyBone regenerationCalcium phosphateFluorideHydroxyapatiteDespite advances in bone graft design and surgical techniques, bacterial infection remains a major cause of graft failure, exacerbated by the global rise in antimicrobial resistance. This has intensified the pursuit of antibiotic-free strategies to prevent bacterial colonization. Among these, antibacterial surface nanotopographies have emerged as promising tools, leveraging nanoscale geometries to physically disrupt bacteria upon contact. In this study, we engineered the surface of a calcium phosphate bone graft to confer antimicrobial functionality through a dual approach: the creation of high-aspect-ratio nanotopographies and ionic doping with fluoride. Through controlled hydrolysis of a-tricalcium phosphate by biomimetic and hydrothermal treatments, we generated calcium deficient hydroxyapatite nanoneedle structures whose morphology and biofunctionality were tuned via fluoride incorporation. XRD and Raman spectroscopy confirmed the formation of hydroxy-fluorapatite, with phase composition and surface morphology dependent on fluoride concentration and processing parameters. Fluoride doping significantly altered nanoneedle dimensions and spacing and enhanced bactericidal activity, particularly against P. aeruginosa, and to a lesser extent S. aureus. Notably, fluoride-doping alone showed no antibacterial effects; however, when combined with nanotopography, a synergistic increase in efficacy was observed. Importantly, the antimicrobial surfaces supported the proliferation and osteogenic differentiation of SaOS-2 cells. Co-culture assays modeling pre- and post-implantation infection scenarios demonstrated robust cell adhesion and markedly reduced bacterial colonization. In conclusion, our findings present a multifunctional, synthetic bone graft with both physical and chemical antibacterial properties, offering a promising strategy to mitigate infection risks while supporting osteointegration.KEAI PUBLISHING LTD2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29717Bioactive MaterialsISSN: 2452199Xreponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p297172026-05-27T12:37:41Z
dc.title.none.fl_str_mv Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
title Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
spellingShingle Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
Arca-Garcia C
Antibacterial nanotopography
Bone regeneration
Calcium phosphate
Fluoride
Hydroxyapatite
title_short Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
title_full Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
title_fullStr Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
title_full_unstemmed Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
title_sort Tailoring nanotopography and antibacterial properties of calcium phosphate bone grafts via fluoride incorporation.
dc.creator.none.fl_str_mv Arca-Garcia C
Godoy-Gallardo M
Ginebra MP
author Arca-Garcia C
author_facet Arca-Garcia C
Godoy-Gallardo M
Ginebra MP
author_role author
author2 Godoy-Gallardo M
Ginebra MP
author2_role author
author
dc.subject.none.fl_str_mv Antibacterial nanotopography
Bone regeneration
Calcium phosphate
Fluoride
Hydroxyapatite
topic Antibacterial nanotopography
Bone regeneration
Calcium phosphate
Fluoride
Hydroxyapatite
description Despite advances in bone graft design and surgical techniques, bacterial infection remains a major cause of graft failure, exacerbated by the global rise in antimicrobial resistance. This has intensified the pursuit of antibiotic-free strategies to prevent bacterial colonization. Among these, antibacterial surface nanotopographies have emerged as promising tools, leveraging nanoscale geometries to physically disrupt bacteria upon contact. In this study, we engineered the surface of a calcium phosphate bone graft to confer antimicrobial functionality through a dual approach: the creation of high-aspect-ratio nanotopographies and ionic doping with fluoride. Through controlled hydrolysis of a-tricalcium phosphate by biomimetic and hydrothermal treatments, we generated calcium deficient hydroxyapatite nanoneedle structures whose morphology and biofunctionality were tuned via fluoride incorporation. XRD and Raman spectroscopy confirmed the formation of hydroxy-fluorapatite, with phase composition and surface morphology dependent on fluoride concentration and processing parameters. Fluoride doping significantly altered nanoneedle dimensions and spacing and enhanced bactericidal activity, particularly against P. aeruginosa, and to a lesser extent S. aureus. Notably, fluoride-doping alone showed no antibacterial effects; however, when combined with nanotopography, a synergistic increase in efficacy was observed. Importantly, the antimicrobial surfaces supported the proliferation and osteogenic differentiation of SaOS-2 cells. Co-culture assays modeling pre- and post-implantation infection scenarios demonstrated robust cell adhesion and markedly reduced bacterial colonization. In conclusion, our findings present a multifunctional, synthetic bone graft with both physical and chemical antibacterial properties, offering a promising strategy to mitigate infection risks while supporting osteointegration.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29717
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=29717
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv KEAI PUBLISHING LTD
publisher.none.fl_str_mv KEAI PUBLISHING LTD
dc.source.none.fl_str_mv Bioactive Materials
ISSN: 2452199X
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
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