Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats

Long-termchronic intermittent exposure to altitude hypoxia is a labor phenomenon requiring further research. Using a rat model, we examined whether this type of exposure differed from chronic exposure in terms of pulmonary artery remodeling and other features. Rats were subjected to chronic hypoxia...

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Autores: Brito, Julio P., Siques, Patricia, Arribas Rodríguez, Silvia Magdalena, López de Pablo León, Ángel Luis, González Enguita, María del Carmen, Naveas, Nelson, Arriaza, Karem, Flores, Karen, León-Velarde, Fabiola, Pulido, Ruth, Ordenes, Stefany, López, M. Rosario
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/671444
Acceso en línea:http://hdl.handle.net/10486/671444
https://dx.doi.org/10.1155/2015/169841
Access Level:acceso abierto
Palabra clave:Hipoxia
Pulmonary artery
Medicina
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spelling Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in ratsBrito, Julio P.Siques, PatriciaArribas Rodríguez, Silvia MagdalenaLópez de Pablo León, Ángel LuisGonzález Enguita, María del CarmenNaveas, NelsonArriaza, KaremFlores, KarenLeón-Velarde, FabiolaPulido, RuthOrdenes, StefanyLópez, M. RosarioHipoxiaPulmonary arteryMedicinaLong-termchronic intermittent exposure to altitude hypoxia is a labor phenomenon requiring further research. Using a rat model, we examined whether this type of exposure differed from chronic exposure in terms of pulmonary artery remodeling and other features. Rats were subjected to chronic hypoxia (CH, = 9) and long-term intermittent hypoxia (CIH2x2; 2 days of hypoxia/2 days of normoxia, = 10) in a chamber (428 Torr, 4,600m of altitude) for 46 days and compared to rats under normoxia (NX, = 10). Body weight, hematocrit, and right ventricle ratio were measured. Pulmonary artery remodeling was assessed using confocal microscopy of tissues stained with a nuclear dye (DAPI) and CD11b antibody. Both hypoxic conditions exhibited increased hematocrit and hypertrophy of the right ventricle, tunica adventitia, and tunica media, with no changes in lumen size. The medial hypertrophy area (larger in CH) depicted a significant increase in smooth muscle cell number. Additionally, CIH2x2 increased the adventitial hypertrophy area, with an increased cellularity and a larger prevalence of clustered inflammatory cells. In conclusion, CIH2x2 elicitsmilder effects on pulmonary artery medial layermuscularization and subsequent right ventricular hypertrophy than CH. However, CIH2x2 induces greater and characteristic alterations of the adventitial layerThis work was funded by GORE-FNDR-BIP: 30125349-0, AECID Nº A/030023/10, and CYTED-ALTMEDFIS grantsHindawi Publishing CorporationDepartamento de FisiologíaDepartamento de Medicina Preventiva y Salud Pública y MicrobiologíaFacultad de Medicina20152015-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/671444https://dx.doi.org/10.1155/2015/169841reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6714442026-06-23T12:46:27Z
dc.title.none.fl_str_mv Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
title Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
spellingShingle Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
Brito, Julio P.
Hipoxia
Pulmonary artery
Medicina
title_short Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
title_full Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
title_fullStr Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
title_full_unstemmed Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
title_sort Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
dc.creator.none.fl_str_mv Brito, Julio P.
Siques, Patricia
Arribas Rodríguez, Silvia Magdalena
López de Pablo León, Ángel Luis
González Enguita, María del Carmen
Naveas, Nelson
Arriaza, Karem
Flores, Karen
León-Velarde, Fabiola
Pulido, Ruth
Ordenes, Stefany
López, M. Rosario
author Brito, Julio P.
author_facet Brito, Julio P.
Siques, Patricia
Arribas Rodríguez, Silvia Magdalena
López de Pablo León, Ángel Luis
González Enguita, María del Carmen
Naveas, Nelson
Arriaza, Karem
Flores, Karen
León-Velarde, Fabiola
Pulido, Ruth
Ordenes, Stefany
López, M. Rosario
author_role author
author2 Siques, Patricia
Arribas Rodríguez, Silvia Magdalena
López de Pablo León, Ángel Luis
González Enguita, María del Carmen
Naveas, Nelson
Arriaza, Karem
Flores, Karen
León-Velarde, Fabiola
Pulido, Ruth
Ordenes, Stefany
López, M. Rosario
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Fisiología
Departamento de Medicina Preventiva y Salud Pública y Microbiología
Facultad de Medicina
dc.subject.none.fl_str_mv Hipoxia
Pulmonary artery
Medicina
topic Hipoxia
Pulmonary artery
Medicina
description Long-termchronic intermittent exposure to altitude hypoxia is a labor phenomenon requiring further research. Using a rat model, we examined whether this type of exposure differed from chronic exposure in terms of pulmonary artery remodeling and other features. Rats were subjected to chronic hypoxia (CH, = 9) and long-term intermittent hypoxia (CIH2x2; 2 days of hypoxia/2 days of normoxia, = 10) in a chamber (428 Torr, 4,600m of altitude) for 46 days and compared to rats under normoxia (NX, = 10). Body weight, hematocrit, and right ventricle ratio were measured. Pulmonary artery remodeling was assessed using confocal microscopy of tissues stained with a nuclear dye (DAPI) and CD11b antibody. Both hypoxic conditions exhibited increased hematocrit and hypertrophy of the right ventricle, tunica adventitia, and tunica media, with no changes in lumen size. The medial hypertrophy area (larger in CH) depicted a significant increase in smooth muscle cell number. Additionally, CIH2x2 increased the adventitial hypertrophy area, with an increased cellularity and a larger prevalence of clustered inflammatory cells. In conclusion, CIH2x2 elicitsmilder effects on pulmonary artery medial layermuscularization and subsequent right ventricular hypertrophy than CH. However, CIH2x2 induces greater and characteristic alterations of the adventitial layer
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/671444
https://dx.doi.org/10.1155/2015/169841
url http://hdl.handle.net/10486/671444
https://dx.doi.org/10.1155/2015/169841
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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