Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato

Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods tha...

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Detalles Bibliográficos
Autores: Ferreira, V., González, M., Pianzzola, María Julia, Coll, N.S., Siri, María Inés, Valls i Matheu, Marc
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/180031
Acceso en línea:https://hdl.handle.net/2445/180031
Access Level:acceso abierto
Palabra clave:Patatera
Malalties bacterianes
Genètica vegetal
Potato
Bacterial diseases
Plant genetics
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spelling Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in PotatoFerreira, V.González, M.Pianzzola, María JuliaColl, N.S.Siri, María InésValls i Matheu, MarcPatateraMalalties bacterianesGenètica vegetalPotatoBacterial diseasesPlant geneticsPotato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples.The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs.Springer Science + Business Media2021202220212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfhttps://hdl.handle.net/2445/180031Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1007/978-1-0716-1609-3_18Methods in Molecular Biology, 2021, vol. 2354, p. 375-385https://doi.org/10.1007/978-1-0716-1609-3_18(c) Springer Science + Business Media, 2021info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1800312026-05-29T05:05:01Z
dc.title.none.fl_str_mv Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
title Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
spellingShingle Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
Ferreira, V.
Patatera
Malalties bacterianes
Genètica vegetal
Potato
Bacterial diseases
Plant genetics
title_short Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
title_full Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
title_fullStr Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
title_full_unstemmed Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
title_sort Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato
dc.creator.none.fl_str_mv Ferreira, V.
González, M.
Pianzzola, María Julia
Coll, N.S.
Siri, María Inés
Valls i Matheu, Marc
author Ferreira, V.
author_facet Ferreira, V.
González, M.
Pianzzola, María Julia
Coll, N.S.
Siri, María Inés
Valls i Matheu, Marc
author_role author
author2 González, M.
Pianzzola, María Julia
Coll, N.S.
Siri, María Inés
Valls i Matheu, Marc
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Patatera
Malalties bacterianes
Genètica vegetal
Potato
Bacterial diseases
Plant genetics
topic Patatera
Malalties bacterianes
Genètica vegetal
Potato
Bacterial diseases
Plant genetics
description Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples.The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/180031
url https://hdl.handle.net/2445/180031
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1007/978-1-0716-1609-3_18
Methods in Molecular Biology, 2021, vol. 2354, p. 375-385
https://doi.org/10.1007/978-1-0716-1609-3_18
dc.rights.none.fl_str_mv (c) Springer Science + Business Media, 2021
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Springer Science + Business Media, 2021
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Springer Science + Business Media
publisher.none.fl_str_mv Springer Science + Business Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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