Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles

Gene therapy holds promise to cure various diseases at the fundamental level. For that, efficient carriers are needed for successful gene delivery. Synthetic 'non-viral' vectors, as cationic polymers, are quickly gaining popularity as efficient vectors for transmitting genes. However, they...

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Detalles Bibliográficos
Autores: Gusta, Muriel F., Edel, Michael J., Salazar, Vivian A., Álvarez-Palomo, Belén, Juan, Manel, Broggini, Massimo, Damia, Giovanna, Bigini, Paolo, Corbelli, Alessandro, Fiordaliso, Fabio, Barbul, Alexander, Korenstein, Rafi, Bastús, Neus G., Puntes, Víctor F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/336348
Acceso en línea:http://hdl.handle.net/10261/336348
https://api.elsevier.com/content/abstract/scopus_id/85160049059
Access Level:acceso abierto
Palabra clave:Cationic
Gene therapeutics
Gold nanoparticles
Safety
Transfection
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spelling Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticlesGusta, Muriel F.Edel, Michael J.Salazar, Vivian A.Álvarez-Palomo, BelénJuan, ManelBroggini, MassimoDamia, GiovannaBigini, PaoloCorbelli, AlessandroFiordaliso, FabioBarbul, AlexanderKorenstein, RafiBastús, Neus G.Puntes, Víctor F.CationicGene therapeuticsGold nanoparticlesSafetyTransfectionGene therapy holds promise to cure various diseases at the fundamental level. For that, efficient carriers are needed for successful gene delivery. Synthetic 'non-viral' vectors, as cationic polymers, are quickly gaining popularity as efficient vectors for transmitting genes. However, they suffer from high toxicity associated with the permeation and poration of the cell membrane. This toxic aspect can be eliminated by nanoconjugation. Still, results suggest that optimising the oligonucleotide complexation, ultimately determined by the size and charge of the nanovector, is not the only barrier to efficient gene delivery.We acknowledge financial support from the Spanish Ministerio de Ciencia, Innovación y Universidades (MCIU) (RTI2018-099965-B-I00, AEI/FEDER,UE) proyectos de I+D+i de programación conjunta internacional MCIN/AEI (CONCORD, PCI2019-103436) cofunded by the European Union and Generalitat de Catalunya (2017-SGR-1431). ICN2 is supported by the Severo Ochoa program from Spanish MINECO (SEV-2017-0706) and is funded by the CERCA Programme/Generalitat de Catalunya.Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Generalitat de CatalunyaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/336348https://api.elsevier.com/content/abstract/scopus_id/85160049059reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099965-B-I00info:eu-repo/grantAgreement/AEI//SEV-2017-0706The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3389/fimmu.2023.1128582https://doi.org/10.3389/fimmu.2023.1128582Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3363482026-05-22T06:33:51Z
dc.title.none.fl_str_mv Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
title Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
spellingShingle Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
Gusta, Muriel F.
Cationic
Gene therapeutics
Gold nanoparticles
Safety
Transfection
title_short Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
title_full Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
title_fullStr Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
title_full_unstemmed Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
title_sort Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles
dc.creator.none.fl_str_mv Gusta, Muriel F.
Edel, Michael J.
Salazar, Vivian A.
Álvarez-Palomo, Belén
Juan, Manel
Broggini, Massimo
Damia, Giovanna
Bigini, Paolo
Corbelli, Alessandro
Fiordaliso, Fabio
Barbul, Alexander
Korenstein, Rafi
Bastús, Neus G.
Puntes, Víctor F.
author Gusta, Muriel F.
author_facet Gusta, Muriel F.
Edel, Michael J.
Salazar, Vivian A.
Álvarez-Palomo, Belén
Juan, Manel
Broggini, Massimo
Damia, Giovanna
Bigini, Paolo
Corbelli, Alessandro
Fiordaliso, Fabio
Barbul, Alexander
Korenstein, Rafi
Bastús, Neus G.
Puntes, Víctor F.
author_role author
author2 Edel, Michael J.
Salazar, Vivian A.
Álvarez-Palomo, Belén
Juan, Manel
Broggini, Massimo
Damia, Giovanna
Bigini, Paolo
Corbelli, Alessandro
Fiordaliso, Fabio
Barbul, Alexander
Korenstein, Rafi
Bastús, Neus G.
Puntes, Víctor F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Generalitat de Catalunya
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cationic
Gene therapeutics
Gold nanoparticles
Safety
Transfection
topic Cationic
Gene therapeutics
Gold nanoparticles
Safety
Transfection
description Gene therapy holds promise to cure various diseases at the fundamental level. For that, efficient carriers are needed for successful gene delivery. Synthetic 'non-viral' vectors, as cationic polymers, are quickly gaining popularity as efficient vectors for transmitting genes. However, they suffer from high toxicity associated with the permeation and poration of the cell membrane. This toxic aspect can be eliminated by nanoconjugation. Still, results suggest that optimising the oligonucleotide complexation, ultimately determined by the size and charge of the nanovector, is not the only barrier to efficient gene delivery.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/336348
https://api.elsevier.com/content/abstract/scopus_id/85160049059
url http://hdl.handle.net/10261/336348
https://api.elsevier.com/content/abstract/scopus_id/85160049059
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099965-B-I00
info:eu-repo/grantAgreement/AEI//SEV-2017-0706
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3389/fimmu.2023.1128582
https://doi.org/10.3389/fimmu.2023.1128582
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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