Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie

Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitte...

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Autores: Garza, María Carmen, Fernández Borges, Natalia, Bolea, Rosa, Badiola, Juan José, Castilla, Joaquín, Monleón Moscardó, Eva
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/45592
Acceso en línea:https://doi.org/10.1371/journal.pone.0027525
http://hdl.handle.net/10459.1/45592
Access Level:acceso abierto
Palabra clave:Encefalopatia espongiforme bovina
Sistema nerviós central -- Malalties
Virosi
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spelling Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapieGarza, María CarmenFernández Borges, NataliaBolea, RosaBadiola, Juan JoséCastilla, JoaquínMonleón Moscardó, EvaEncefalopatia espongiforme bovinaSistema nerviós central -- MalaltiesVirosiScrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.This study was supported by a national grant from Spain (AGL2009-11553-C02-01), a Basque government grant (PI2010-18) and the Department of Industry, Tourism and Trade of the Government of the Autonomous Community of the Basque Country (Etortek Research Programs 2011/2013), from the Innovation Technology Department of the Bizkaia County. Dr. Garza was supported by a FPU doctoral grant from the Spanish Ministry of Education (AP2007-03842). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of Science (PLoS)2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1371/journal.pone.0027525http://hdl.handle.net/10459.1/45592reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/MICINN//AGL2009-11553-C02-01Reproducció del document publicat a https://doi.org/10.1371/journal.pone.0027525PLoS ONE, 2011, vol. 6, núm. 12, e27525cc-by, (c) Garza et al., 2011info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/es/deed.caoai:repositori.udl.cat:10459.1/455922026-06-24T12:42:17Z
dc.title.none.fl_str_mv Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
spellingShingle Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
Garza, María Carmen
Encefalopatia espongiforme bovina
Sistema nerviós central -- Malalties
Virosi
title_short Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_full Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_fullStr Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_full_unstemmed Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
title_sort Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie
dc.creator.none.fl_str_mv Garza, María Carmen
Fernández Borges, Natalia
Bolea, Rosa
Badiola, Juan José
Castilla, Joaquín
Monleón Moscardó, Eva
author Garza, María Carmen
author_facet Garza, María Carmen
Fernández Borges, Natalia
Bolea, Rosa
Badiola, Juan José
Castilla, Joaquín
Monleón Moscardó, Eva
author_role author
author2 Fernández Borges, Natalia
Bolea, Rosa
Badiola, Juan José
Castilla, Joaquín
Monleón Moscardó, Eva
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Encefalopatia espongiforme bovina
Sistema nerviós central -- Malalties
Virosi
topic Encefalopatia espongiforme bovina
Sistema nerviós central -- Malalties
Virosi
description Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1371/journal.pone.0027525
http://hdl.handle.net/10459.1/45592
url https://doi.org/10.1371/journal.pone.0027525
http://hdl.handle.net/10459.1/45592
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2009-11553-C02-01
Reproducció del document publicat a https://doi.org/10.1371/journal.pone.0027525
PLoS ONE, 2011, vol. 6, núm. 12, e27525
dc.rights.none.fl_str_mv cc-by, (c) Garza et al., 2011
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/es/deed.ca
rights_invalid_str_mv cc-by, (c) Garza et al., 2011
http://creativecommons.org/licenses/by/2.5/es/deed.ca
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
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repository.mail.fl_str_mv
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