Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope

Cyclin-dependent kinases (CDKs) play key roles in cell cycle regulation. Genetic analysis in mice has revealed an essential role for Cdk2 in meiosis, which renders Cdk2 knockout (KO) mice sterile. Here we show that mice deficient in RingoA, an atypical activator of Cdk1 and Cdk2 that has no amino ac...

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Autores: Mikolcevic, Petra, Isoda, Michitaka, Shibuya, Hiroki, Barco Barrantes, Ivan del, Igea, Ana, Suja, José .A., Shackleton, Sue, Watanabe, Yoshinori, Nebreda, Àngel R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/105640
Acceso en línea:https://hdl.handle.net/2445/105640
Access Level:acceso abierto
Palabra clave:Interacció cel·lular
Meiosi
Cell interaction
Meiosis
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spelling Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelopeMikolcevic, PetraIsoda, MichitakaShibuya, HirokiBarco Barrantes, Ivan delIgea, AnaSuja, José .A.Shackleton, SueWatanabe, YoshinoriNebreda, Àngel R.Interacció cel·lularMeiosiCell interactionMeiosisCyclin-dependent kinases (CDKs) play key roles in cell cycle regulation. Genetic analysis in mice has revealed an essential role for Cdk2 in meiosis, which renders Cdk2 knockout (KO) mice sterile. Here we show that mice deficient in RingoA, an atypical activator of Cdk1 and Cdk2 that has no amino acid sequence homology to cyclins, are sterile and display meiotic defects virtually identical to those observed in Cdk2 KO mice including non-homologous chromosome pairing, unrepaired double-strand breaks, undetectable sex-body and pachytene arrest. Interestingly, RingoA is required for Cdk2 targeting to telomeres and RingoA KO spermatocytes display severely affected telomere tethering as well as impaired distribution of Sun1, a protein essential for the attachment of telomeres to the nuclear envelope. Our results identify RingoA as an important activator of Cdk2 at meiotic telomeres, and provide genetic evidence for a physiological function of mammalian Cdk2 that is not dependent on cyclins.Macmillan2017201720162017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/105640Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1038/ncomms11084Nature Communications, 2016, vol. 7, p. 11084http://dx.doi.org/10.1038/ncomms11084cc by (c) Mikolcevic et al., 2017http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1056402026-05-29T05:05:01Z
dc.title.none.fl_str_mv Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
title Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
spellingShingle Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
Mikolcevic, Petra
Interacció cel·lular
Meiosi
Cell interaction
Meiosis
title_short Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
title_full Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
title_fullStr Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
title_full_unstemmed Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
title_sort Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
dc.creator.none.fl_str_mv Mikolcevic, Petra
Isoda, Michitaka
Shibuya, Hiroki
Barco Barrantes, Ivan del
Igea, Ana
Suja, José .A.
Shackleton, Sue
Watanabe, Yoshinori
Nebreda, Àngel R.
author Mikolcevic, Petra
author_facet Mikolcevic, Petra
Isoda, Michitaka
Shibuya, Hiroki
Barco Barrantes, Ivan del
Igea, Ana
Suja, José .A.
Shackleton, Sue
Watanabe, Yoshinori
Nebreda, Àngel R.
author_role author
author2 Isoda, Michitaka
Shibuya, Hiroki
Barco Barrantes, Ivan del
Igea, Ana
Suja, José .A.
Shackleton, Sue
Watanabe, Yoshinori
Nebreda, Àngel R.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Interacció cel·lular
Meiosi
Cell interaction
Meiosis
topic Interacció cel·lular
Meiosi
Cell interaction
Meiosis
description Cyclin-dependent kinases (CDKs) play key roles in cell cycle regulation. Genetic analysis in mice has revealed an essential role for Cdk2 in meiosis, which renders Cdk2 knockout (KO) mice sterile. Here we show that mice deficient in RingoA, an atypical activator of Cdk1 and Cdk2 that has no amino acid sequence homology to cyclins, are sterile and display meiotic defects virtually identical to those observed in Cdk2 KO mice including non-homologous chromosome pairing, unrepaired double-strand breaks, undetectable sex-body and pachytene arrest. Interestingly, RingoA is required for Cdk2 targeting to telomeres and RingoA KO spermatocytes display severely affected telomere tethering as well as impaired distribution of Sun1, a protein essential for the attachment of telomeres to the nuclear envelope. Our results identify RingoA as an important activator of Cdk2 at meiotic telomeres, and provide genetic evidence for a physiological function of mammalian Cdk2 that is not dependent on cyclins.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/105640
url https://hdl.handle.net/2445/105640
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1038/ncomms11084
Nature Communications, 2016, vol. 7, p. 11084
http://dx.doi.org/10.1038/ncomms11084
dc.rights.none.fl_str_mv cc by (c) Mikolcevic et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Mikolcevic et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Macmillan
publisher.none.fl_str_mv Macmillan
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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