Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus

The Kv4 family of voltage-gated K+ channels underlie the fast transient (A-type) outward K+ current. Although A-type currents are critical to determine somato-dendritic integration in central neurons, relatively little is known about the precise subcellular localisation of the underlying channels in...

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Autores: Alfaro Ruiz, Rocío, Aguado Rubio, Carolina, Martín Belmonte, Alejandro, Moreno Martínez, Ana Esther, Luján Miras, Rafael
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/23738
Acceso en línea:http://dx.doi.org/10.3390/ijms20020246
https://hdl.handle.net/10578/23738
Access Level:acceso abierto
Palabra clave:Hippocampus
Potassium channel
Electron microscopy
Immunohistohemistry
Histoblot
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spelling Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent HippocampusAlfaro Ruiz, RocíoAguado Rubio, CarolinaMartín Belmonte, AlejandroMoreno Martínez, Ana EstherLuján Miras, RafaelHippocampusPotassium channelElectron microscopyImmunohistohemistryHistoblotThe Kv4 family of voltage-gated K+ channels underlie the fast transient (A-type) outward K+ current. Although A-type currents are critical to determine somato-dendritic integration in central neurons, relatively little is known about the precise subcellular localisation of the underlying channels in hippocampal circuits. Using histoblot and immunoelectron microscopic techniques, we investigated the expression, regional distribution and subcellular localisation of Kv4.2 and Kv4.3 in the adult brain, as well as the ontogeny of their expression during postnatal development. Histoblot demonstrated that Kv4.2 and Kv4.3 proteins were widely expressed in the brain, with mostly non-overlapping patterns. During development, levels of Kv4.2 and Kv4.3 increased with age but showed marked region- and developmental stage-specific differences. Immunoelectron microscopy showed that labelling for Kv4.2 and Kv4.3 was differentially present in somato-dendritic domains of hippocampal principal cells and interneurons, including the synaptic specialisation. Quantitative analyses indicated that most immunoparticles for Kv4.2 and Kv4.3 were associated with the plasma membrane in dendritic spines and shafts, and that the two channels showed very similar distribution patterns in spines of principal cells and along the surface of granule cells. Our data shed new light on the subcellular localisation of Kv4 channels and provide evidence for their non-uniform distribution over the plasma membrane of hippocampal neurons.MDPI202020202019info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://dx.doi.org/10.3390/ijms20020246https://hdl.handle.net/10578/23738reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/237382026-05-27T07:36:41Z
dc.title.none.fl_str_mv Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
title Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
spellingShingle Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
Alfaro Ruiz, Rocío
Hippocampus
Potassium channel
Electron microscopy
Immunohistohemistry
Histoblot
title_short Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
title_full Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
title_fullStr Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
title_full_unstemmed Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
title_sort Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
dc.creator.none.fl_str_mv Alfaro Ruiz, Rocío
Aguado Rubio, Carolina
Martín Belmonte, Alejandro
Moreno Martínez, Ana Esther
Luján Miras, Rafael
author Alfaro Ruiz, Rocío
author_facet Alfaro Ruiz, Rocío
Aguado Rubio, Carolina
Martín Belmonte, Alejandro
Moreno Martínez, Ana Esther
Luján Miras, Rafael
author_role author
author2 Aguado Rubio, Carolina
Martín Belmonte, Alejandro
Moreno Martínez, Ana Esther
Luján Miras, Rafael
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hippocampus
Potassium channel
Electron microscopy
Immunohistohemistry
Histoblot
topic Hippocampus
Potassium channel
Electron microscopy
Immunohistohemistry
Histoblot
description The Kv4 family of voltage-gated K+ channels underlie the fast transient (A-type) outward K+ current. Although A-type currents are critical to determine somato-dendritic integration in central neurons, relatively little is known about the precise subcellular localisation of the underlying channels in hippocampal circuits. Using histoblot and immunoelectron microscopic techniques, we investigated the expression, regional distribution and subcellular localisation of Kv4.2 and Kv4.3 in the adult brain, as well as the ontogeny of their expression during postnatal development. Histoblot demonstrated that Kv4.2 and Kv4.3 proteins were widely expressed in the brain, with mostly non-overlapping patterns. During development, levels of Kv4.2 and Kv4.3 increased with age but showed marked region- and developmental stage-specific differences. Immunoelectron microscopy showed that labelling for Kv4.2 and Kv4.3 was differentially present in somato-dendritic domains of hippocampal principal cells and interneurons, including the synaptic specialisation. Quantitative analyses indicated that most immunoparticles for Kv4.2 and Kv4.3 were associated with the plasma membrane in dendritic spines and shafts, and that the two channels showed very similar distribution patterns in spines of principal cells and along the surface of granule cells. Our data shed new light on the subcellular localisation of Kv4 channels and provide evidence for their non-uniform distribution over the plasma membrane of hippocampal neurons.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://dx.doi.org/10.3390/ijms20020246
https://hdl.handle.net/10578/23738
url http://dx.doi.org/10.3390/ijms20020246
https://hdl.handle.net/10578/23738
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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