Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter

Type 1 Diabetes results from autoimmune response elicited against β-cell antigens. Nowadays, insulin injections remain the leading therapeutic option. However, injection treatment fails to emulate the highly dynamic insulin release that β-cells provide. 3D cell-laden microspheres have been proposed...

Descripción completa

Detalles Bibliográficos
Autores: Clua Ferré, Laura, De Chiara, Francesco, Rodríguez Comas, Júlia, Comelles Pujadas, Jordi, Martínez Fraiz, Elena, Godeau, Amelie Luise, García Alamán, Ainhoa, Gasa, Rosa, Ramón Azcón, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/183436
Acceso en línea:https://hdl.handle.net/2445/183436
Access Level:acceso abierto
Palabra clave:Enginyeria biomèdica
Diabetis
Materials biomèdics
Biomedical engineering
Diabetes
Biomedical materials
id ES_ef6126cd1c27e6c7d9eb774012be2ea3
oai_identifier_str oai:diposit.ub.edu:2445/183436
network_acronym_str ES
network_name_str España
repository_id_str
spelling Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D BioprinterClua Ferré, LauraDe Chiara, FrancescoRodríguez Comas, JúliaComelles Pujadas, JordiMartínez Fraiz, ElenaGodeau, Amelie LuiseGarcía Alamán, AinhoaGasa, RosaRamón Azcón, JavierEnginyeria biomèdicaDiabetisMaterials biomèdicsBiomedical engineeringDiabetesBiomedical materialsType 1 Diabetes results from autoimmune response elicited against β-cell antigens. Nowadays, insulin injections remain the leading therapeutic option. However, injection treatment fails to emulate the highly dynamic insulin release that β-cells provide. 3D cell-laden microspheres have been proposed during the last years as a major platform for bioengineering insulin-secreting constructs for tissue graft implantation and a model for in vitro drug screening platforms. Current microsphere fabrication technologies have several drawbacks: the need for an oil phase containing surfactants, diameter inconsistency of the microspheres, and high time-consuming processes. These technologies have widely used alginate for its rapid gelation, high processability, and low cost. However, its low biocompatible properties do not provide effective cell attachment. This study proposes a high-throughput methodology using a 3D bioprinter that employs an ECM-like microenvironment for effective cell-laden microsphere production to overcome these limitations. Crosslinking the resulting microspheres with tannic acid prevents collagenase degradation and enhances spherical structural consistency while allowing the diffusion of nutrients and oxygen. The approach allows customization of microsphere diameter with extremely low variability. In conclusion, a novel bio-printing procedure is developed to fabricate large amounts of reproducible microspheres capable of secreting insulin in response to extracellular glucose stimuli.Wiley2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/183436Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1002/admt.202101696Advanced Materials Technologies, 2022, num. 2101696https://doi.org/10.1002/admt.202101696info:eu-repo/grantAgreement/EC/H2020/714317cc by-nc (c) Clua Ferré, Laura et al., 2022http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1834362026-05-27T06:46:51Z
dc.title.none.fl_str_mv Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
title Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
spellingShingle Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
Clua Ferré, Laura
Enginyeria biomèdica
Diabetis
Materials biomèdics
Biomedical engineering
Diabetes
Biomedical materials
title_short Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
title_full Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
title_fullStr Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
title_full_unstemmed Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
title_sort Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
dc.creator.none.fl_str_mv Clua Ferré, Laura
De Chiara, Francesco
Rodríguez Comas, Júlia
Comelles Pujadas, Jordi
Martínez Fraiz, Elena
Godeau, Amelie Luise
García Alamán, Ainhoa
Gasa, Rosa
Ramón Azcón, Javier
author Clua Ferré, Laura
author_facet Clua Ferré, Laura
De Chiara, Francesco
Rodríguez Comas, Júlia
Comelles Pujadas, Jordi
Martínez Fraiz, Elena
Godeau, Amelie Luise
García Alamán, Ainhoa
Gasa, Rosa
Ramón Azcón, Javier
author_role author
author2 De Chiara, Francesco
Rodríguez Comas, Júlia
Comelles Pujadas, Jordi
Martínez Fraiz, Elena
Godeau, Amelie Luise
García Alamán, Ainhoa
Gasa, Rosa
Ramón Azcón, Javier
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enginyeria biomèdica
Diabetis
Materials biomèdics
Biomedical engineering
Diabetes
Biomedical materials
topic Enginyeria biomèdica
Diabetis
Materials biomèdics
Biomedical engineering
Diabetes
Biomedical materials
description Type 1 Diabetes results from autoimmune response elicited against β-cell antigens. Nowadays, insulin injections remain the leading therapeutic option. However, injection treatment fails to emulate the highly dynamic insulin release that β-cells provide. 3D cell-laden microspheres have been proposed during the last years as a major platform for bioengineering insulin-secreting constructs for tissue graft implantation and a model for in vitro drug screening platforms. Current microsphere fabrication technologies have several drawbacks: the need for an oil phase containing surfactants, diameter inconsistency of the microspheres, and high time-consuming processes. These technologies have widely used alginate for its rapid gelation, high processability, and low cost. However, its low biocompatible properties do not provide effective cell attachment. This study proposes a high-throughput methodology using a 3D bioprinter that employs an ECM-like microenvironment for effective cell-laden microsphere production to overcome these limitations. Crosslinking the resulting microspheres with tannic acid prevents collagenase degradation and enhances spherical structural consistency while allowing the diffusion of nutrients and oxygen. The approach allows customization of microsphere diameter with extremely low variability. In conclusion, a novel bio-printing procedure is developed to fabricate large amounts of reproducible microspheres capable of secreting insulin in response to extracellular glucose stimuli.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/183436
url https://hdl.handle.net/2445/183436
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1002/admt.202101696
Advanced Materials Technologies, 2022, num. 2101696
https://doi.org/10.1002/admt.202101696
info:eu-repo/grantAgreement/EC/H2020/714317
dc.rights.none.fl_str_mv cc by-nc (c) Clua Ferré, Laura et al., 2022
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc (c) Clua Ferré, Laura et al., 2022
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423857718263808
score 15,300724

Similares en LA Referencia