Plasma Viral Mirnas Indicate A High Prevalence Of Occult Viral Infections

Prevalence of Kaposi sarcoma-associated herpesvirus (KSHV/HHV-8) varies greatly in different populations. We hypothesized that the actual prevalence of KSHV/HHV8 infection in humans is underestimated by the currently available serological tests. We analyzed four independent patient cohorts with post...

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Detalles Bibliográficos
Autores: Fuentes Mattei, Enrique, Giza, Dana Elena, Shimizu, Masayoshi, Ivan, Cristina, Manning, John T., Tudor, Stefan, Ciccone, Maria, Kargin, Osman Aykan, Zhang, Xinna, Mur, Pilar, Amaral, Nayra Soares do, Chen, Meng, Tarrand, Jeffrey J., Lupu, Florea, Ferrajoli, Alessandra, Keating, Michael J., Vasilescu, Catalin, Yeung, Sai-Ching Jim, Calin, George A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/124389
Acceso en línea:https://hdl.handle.net/2445/124389
Access Level:acceso abierto
Palabra clave:Herpesvirus
Micro RNAs
Herpesviruses
MicroRNAs
Descripción
Sumario:Prevalence of Kaposi sarcoma-associated herpesvirus (KSHV/HHV-8) varies greatly in different populations. We hypothesized that the actual prevalence of KSHV/HHV8 infection in humans is underestimated by the currently available serological tests. We analyzed four independent patient cohorts with post-surgical or post-chemotherapy sepsis, chronic lymphocytic leukemia and post-surgical patients with abdominal surgical interventions. Levels of specific KSHV-encoded miRNAs were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and KSHV/HHV-8 IgG were measured by immunoassay. We also measured specific miRNAs from Epstein Barr Virus (EBV), a virus closely related to KSHV/HHV-8, and determined the EBV serological status by ELISA for Epstein-Barr nuclear antigen 1 (EBNA-1) IgG. Finally, we identified the viral miRNAs by in situ hybridization (ISH) in bone marrow cells. In training/validation settings using independent multi-institutional cohorts of 300 plasma samples, we identified in 78.50% of the samples detectable expression of at least one of the three tested KSHV-miRNAs by RT-qPCR, while only 27.57% of samples were found to be seropositive for KSHV/HHV-8 IgG (P < 0.001). The prevalence of KSHV infection based on miRNAs qPCR is significantly higher than the prevalence determined by seropositivity, and this ismore obvious for immuno-depressed patients. Plasma viral miRNAs quantification proved that EBV infection is ubiquitous. Measurement of viral miRNAs by qPCR has the potential to become the "gold" standard method to detect certain viral infections in clinical practice. (C) 2017 Elsevier B. V.

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