Rye Straw Lignin as a Promising Source of Tricin: Varietal Differences and Its Release Using Deep Eutectic Solvents

Lignins isolated from the straw of six rye (Secale cereale) varieties (Cl 98, Slapske, Zidlochovicke Panis, Ceske Normalni, Bates, and SU Stakkato) were comprehensively characterized by advanced analytical techniques to assess their structural features and valorization potential. Rye straws containe...

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Bibliographic Details
Authors: Benito Jareño, Javier, Cañadas, Raquel, Barro Losada, Francisco, Gutiérrez Suárez, Ana, da Costa Lopes, André M., Seixas, Nalin, Santos, Sónia A.O., Silvestre, Armando J.D., Río Andrade, José Carlos del, Rencoret, Jorge
Format: conjunto de datos
Publication Date:2026
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::eea5526467d934e431229f864899d9d2
Online Access:http://hdl.handle.net/10261/428078
Access Level:Open access
Keyword:Advanced analytical techniques
Value tricin recovery
Rye straw lignin
Zidlochovicke panis
Varietal differences
Sustainable source
Structutal features
Selective release
Secale cereale
Renewable biopolymer
Promising source
Primarily constituted
Minor amounts
Lignin backbone
Lactic acid
Kg straw
G straw
Free tricin
Efficiently cleave
Effective cleavage
Choline chloride
Ceske normalni
Description
Summary:Lignins isolated from the straw of six rye (Secale cereale) varieties (Cl 98, Slapske, Zidlochovicke Panis, Ceske Normalni, Bates, and SU Stakkato) were comprehensively characterized by advanced analytical techniques to assess their structural features and valorization potential. Rye straws contained ∼15% of lignin, which was primarily constituted by guaiacyl (∼52–61%) and syringyl (∼35–45%) units, with minor amounts of p-hydroxyphenyl units (∼3%). The lignin backbone was enriched in β–O–4′ alkyl–aryl ether linkages (77–79%), followed by β–5′ (10–12%) and β–β′ (4–6%). All the lignins from the rye straws were enriched in tricin, with the Cl 98 variety having the highest content, reaching up to 26.2 g/kg straw. Since tricin is incorporated exclusively through 4′–O–β ether linkages, an environmentally benign deep eutectic solvent (DES, choline chloride/lactic acid, 1:10), which is known to efficiently cleave such bonds, was evaluated for its selective release. DES treatment of Cl 98 straw yielded 1.92 mg of free tricin/g straw, confirming the effective cleavage of β–O–4 linkages. This study highlights rye straw lignin as a tricin-enriched, renewable biopolymer, emphasizing its potential as a sustainable source for high-value tricin recovery and supporting the valorization of cereal crop residues within lignocellulosic biorefineries.