Pseudomonas fitomaticsae sp. nov., isolated at Marimurtra Botanical Garden in Blanes, Catalonia, Spain
In the framework of the research project called fitomatics, we have isolated and characterized a bacterial plant-endophyte from the rhizomes of Iris germanica, hereafter referred to as strain FIT81. The bacterium is Gram negative, rod-shaped with lophotrichous flagella, and catalase-and oxidase-posi...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:273536 |
| Acceso en línea: | https://ddd.uab.cat/record/273536 https://dx.doi.org/urn:doi:10.1099/IJSEM.0.005557 |
| Access Level: | acceso abierto |
| Palabra clave: | Genome assembly Genome-to-genome comparison Multilocus sequence analysis Pseudomonas fitomaticsae FIT81T |
| Sumario: | In the framework of the research project called fitomatics, we have isolated and characterized a bacterial plant-endophyte from the rhizomes of Iris germanica, hereafter referred to as strain FIT81. The bacterium is Gram negative, rod-shaped with lophotrichous flagella, and catalase-and oxidase-positive. The optimal growth temperature of strain FIT81 is 28 °C, although it can grow within a temperature range of 4-32 °C. The pH growth tolerance ranges between pH 5 and 10, and it tolerates 4% (w/v) NaCl. A 16S rRNA phylogenetic analysis positioned strain FIT81 within the genus Pseudomonas, and multilocus sequence analysis revealed that Pseudomonas gozinkensis IzPS32d, Pseudomonas glycinae MS586, Pseudomonas allokribbensis IzPS23, 'Pseudomonas kribbensis' 46-2 and Pseudomonas koreensis PS9-14 are the top five most closely related species, which were selected for further genome-to-genome comparisons, as well as for physiological and chemotaxonomic characterization. The genome size of strain FIT81 is 6 492 796 base-pairs long, with 60.6 mol% of G+C content. Average nucleotide identity and digital DNA-DNA hybridization analyses yielded values of 93.6 and 56.1%, respectively, when the FIT81 genome was compared to that of the closest type strain P. gozinkensis IzPS32d. Taken together, the obtained genomic, physiologic and chemotaxonomic data indicate that strain FIT81 is different from its closest relative species, which lead us to suggest that it is a novel species to be included in the list of type strains with the name Pseudomonas fitomaticsae sp. nov. (FIT81=CECT 30374=DSM 112699). |
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