Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli

21 Pág.

Detalles Bibliográficos
Autores: Díaz, Silvia, Aguilera, Ángeles, de Figueras, Carolina G., De Francisco, Patricia, Olsson, Sanna, Puente-Sánchez, Fernando, González-Pastor, José Eduardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/278865
Acceso en línea:http://hdl.handle.net/10261/278865
https://api.elsevier.com/content/abstract/scopus_id/85132587492
Access Level:acceso abierto
Palabra clave:Bioaccumulation
Heavy metal resistance
Oxidative stress
Phytochelatins
Stress response
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spelling Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coliDíaz, SilviaAguilera, Ángelesde Figueras, Carolina G.De Francisco, PatriciaOlsson, SannaPuente-Sánchez, FernandoGonzález-Pastor, José EduardoBioaccumulationHeavy metal resistanceOxidative stressPhytochelatinsStress response21 Pág.Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation.This research was funded by grant numbers RTI2018-094867-B-I00, PID2019-104205GB-C22, and PGC2018-096956-B-C42 from the Spanish Ministry of Science and Innovation/ Agency of Research MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Díaz, Silvia [0000-0002-9230-7055]Aguilera, Ángeles [0000-0003-4979-7578]de Figueras, Carolina G [0000-0002-7867-2419]Olsson, Sanna [0000-0002-1199-4499]Puente-Sánchez, Fernando [0000-0002-6341-3692]González-Pastor, José Eduardo [0000-0002-7615-7042]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/278865https://api.elsevier.com/content/abstract/scopus_id/85132587492reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094867-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104205GB-C22info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-096956-B-C42Centro de Investigación Forestal (CIFOR)https://doi.org/10.3390/ijerph19137692Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2788652026-05-22T06:33:51Z
dc.title.none.fl_str_mv Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
spellingShingle Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
Díaz, Silvia
Bioaccumulation
Heavy metal resistance
Oxidative stress
Phytochelatins
Stress response
title_short Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_full Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_fullStr Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_full_unstemmed Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
title_sort Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
dc.creator.none.fl_str_mv Díaz, Silvia
Aguilera, Ángeles
de Figueras, Carolina G.
De Francisco, Patricia
Olsson, Sanna
Puente-Sánchez, Fernando
González-Pastor, José Eduardo
author Díaz, Silvia
author_facet Díaz, Silvia
Aguilera, Ángeles
de Figueras, Carolina G.
De Francisco, Patricia
Olsson, Sanna
Puente-Sánchez, Fernando
González-Pastor, José Eduardo
author_role author
author2 Aguilera, Ángeles
de Figueras, Carolina G.
De Francisco, Patricia
Olsson, Sanna
Puente-Sánchez, Fernando
González-Pastor, José Eduardo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Díaz, Silvia [0000-0002-9230-7055]
Aguilera, Ángeles [0000-0003-4979-7578]
de Figueras, Carolina G [0000-0002-7867-2419]
Olsson, Sanna [0000-0002-1199-4499]
Puente-Sánchez, Fernando [0000-0002-6341-3692]
González-Pastor, José Eduardo [0000-0002-7615-7042]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bioaccumulation
Heavy metal resistance
Oxidative stress
Phytochelatins
Stress response
topic Bioaccumulation
Heavy metal resistance
Oxidative stress
Phytochelatins
Stress response
description 21 Pág.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/278865
https://api.elsevier.com/content/abstract/scopus_id/85132587492
url http://hdl.handle.net/10261/278865
https://api.elsevier.com/content/abstract/scopus_id/85132587492
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094867-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104205GB-C22
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-096956-B-C42
Centro de Investigación Forestal (CIFOR)
https://doi.org/10.3390/ijerph19137692
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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