Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction

Molina-de la Fuente, Irene; Pacheco, M Andreína; Garcia, Luz; Gonzalez-Mora, Vicenta; Riloha, Matilde; Oki, Consuelo; Benito, Agustin; Escalante, Ananias A; Berzosa, Pedro

Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction

Background: Pfhrp2 and pfhrp3 deletions are threatening Plasmodium falciparum malaria diagnosis by rapid diagnostic tests (RDT) due to false negatives. This study assesses the changes in the frequencies of pfhrp2 and pfhrp3 deletions (pfhrp2Del and pfhrp3Del, respectively) and the genes in their fla...

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Detalhes bibliográficos
Autores:	Molina-de la Fuente, Irene, Pacheco, M Andreína, Garcia, Luz, Gonzalez-Mora, Vicenta, Riloha, Matilde, Oki, Consuelo, Benito, Agustin, Escalante, Ananias A, Berzosa, Pedro
Formato:	artículo
Fecha de publicación:	2024
País:	España
Recursos:	Instituto de Salud Carlos III (ISCIII)
Repositorio:	Repisalud
Idioma:	inglés
OAI Identifier:	oai:repisalud.isciii.es:20.500.12105/26264
Acesso em linha:	https://hdl.handle.net/20.500.12105/26264
Access Level:	acceso abierto
Palavra-chave:	Africa False negatives Malaria RDT Subgenus Laverania Antigens, Protozoan Diagnostic Tests, Routine Equatorial Guinea Evolution, Molecular Gene Deletion Humans Malaria, Falciparum Plasmodium falciparum Protozoan Proteins Sequence Deletion

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network_acronym_str	ES
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spelling	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introductionMolina-de la Fuente, IrenePacheco, M AndreínaGarcia, LuzGonzalez-Mora, VicentaRiloha, MatildeOki, ConsueloBenito, AgustinEscalante, Ananias ABerzosa, PedroAfricaFalse negativesMalariaRDTSubgenus LaveraniaAntigens, ProtozoanDiagnostic Tests, RoutineEquatorial GuineaEvolution, MolecularGene DeletionHumansMalaria, FalciparumPlasmodium falciparumProtozoan ProteinsSequence DeletionBackground: Pfhrp2 and pfhrp3 deletions are threatening Plasmodium falciparum malaria diagnosis by rapid diagnostic tests (RDT) due to false negatives. This study assesses the changes in the frequencies of pfhrp2 and pfhrp3 deletions (pfhrp2Del and pfhrp3Del, respectively) and the genes in their flaking regions, before and after RDT introduction in Equatorial Guinea. Methods: A total of 566 P. falciparum samples were genotyped to assess the presence of pfhrp2 and pfhrp3 deletions and their flanking genes. The specimens were collected 18 years apart from two provinces of Equatorial Guinea, North Bioko (Insular Region) and Litoral Province (Continental Region). Orthologs of pfhrp2 and pfhrp3 genes from other closely related species were used to compare sequencing data to assess pfhrp2 and pfhrp3 evolution. Additionally, population structure was studied using seven neutral microsatellites. Results: This study found that pfhrp2Del and pfhrp3Del were present before the introduction of RDT; however, they increased in frequency after their use, reaching more than 15%. Haplotype networks suggested that pfhrp2Del and pfhrp3Del emerged multiple times. Exon 2 of pfhrp2 and pfhrp3 genes had high variability, but there were no significant changes in amino acid sequences. Conclusions: Baseline sampling before deploying interventions provides a valuable context to interpret changes in genetic markers linked to their efficacy, such as the dynamic of deletions affecting RDT efficacy.BioMed Central (BMC)Instituto de Salud Carlos IIIUniversity of Alcalá (España)20252025-02-0520242024-07-1820242024-07-18research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/20.500.12105/26264reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES TRPY447 2021open accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/262642026-06-12T12:43:37Z
dc.title.none.fl_str_mv	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction
title	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction
spellingShingle	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction Molina-de la Fuente, Irene Africa False negatives Malaria RDT Subgenus Laverania Antigens, Protozoan Diagnostic Tests, Routine Equatorial Guinea Evolution, Molecular Gene Deletion Humans Malaria, Falciparum Plasmodium falciparum Protozoan Proteins Sequence Deletion
title_short	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction
title_full	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction
title_fullStr	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction
title_full_unstemmed	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction
title_sort	Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction
dc.creator.none.fl_str_mv	Molina-de la Fuente, Irene Pacheco, M Andreína Garcia, Luz Gonzalez-Mora, Vicenta Riloha, Matilde Oki, Consuelo Benito, Agustin Escalante, Ananias A Berzosa, Pedro
author	Molina-de la Fuente, Irene
author_facet	Molina-de la Fuente, Irene Pacheco, M Andreína Garcia, Luz Gonzalez-Mora, Vicenta Riloha, Matilde Oki, Consuelo Benito, Agustin Escalante, Ananias A Berzosa, Pedro
author_role	author
author2	Pacheco, M Andreína Garcia, Luz Gonzalez-Mora, Vicenta Riloha, Matilde Oki, Consuelo Benito, Agustin Escalante, Ananias A Berzosa, Pedro
author2_role	author author author author author author author author
dc.contributor.none.fl_str_mv	Instituto de Salud Carlos III University of Alcalá (España)
dc.subject.none.fl_str_mv	Africa False negatives Malaria RDT Subgenus Laverania Antigens, Protozoan Diagnostic Tests, Routine Equatorial Guinea Evolution, Molecular Gene Deletion Humans Malaria, Falciparum Plasmodium falciparum Protozoan Proteins Sequence Deletion
topic	Africa False negatives Malaria RDT Subgenus Laverania Antigens, Protozoan Diagnostic Tests, Routine Equatorial Guinea Evolution, Molecular Gene Deletion Humans Malaria, Falciparum Plasmodium falciparum Protozoan Proteins Sequence Deletion
description	Background: Pfhrp2 and pfhrp3 deletions are threatening Plasmodium falciparum malaria diagnosis by rapid diagnostic tests (RDT) due to false negatives. This study assesses the changes in the frequencies of pfhrp2 and pfhrp3 deletions (pfhrp2Del and pfhrp3Del, respectively) and the genes in their flaking regions, before and after RDT introduction in Equatorial Guinea. Methods: A total of 566 P. falciparum samples were genotyped to assess the presence of pfhrp2 and pfhrp3 deletions and their flanking genes. The specimens were collected 18 years apart from two provinces of Equatorial Guinea, North Bioko (Insular Region) and Litoral Province (Continental Region). Orthologs of pfhrp2 and pfhrp3 genes from other closely related species were used to compare sequencing data to assess pfhrp2 and pfhrp3 evolution. Additionally, population structure was studied using seven neutral microsatellites. Results: This study found that pfhrp2Del and pfhrp3Del were present before the introduction of RDT; however, they increased in frequency after their use, reaching more than 15%. Haplotype networks suggested that pfhrp2Del and pfhrp3Del emerged multiple times. Exon 2 of pfhrp2 and pfhrp3 genes had high variability, but there were no significant changes in amino acid sequences. Conclusions: Baseline sampling before deploying interventions provides a valuable context to interpret changes in genetic markers linked to their efficacy, such as the dynamic of deletions affecting RDT efficacy.
publishDate	2024
dc.date.none.fl_str_mv	2024 2024-07-18 2024 2024-07-18 2025 2025-02-05
dc.type.none.fl_str_mv	research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/20.500.12105/26264
url	https://hdl.handle.net/20.500.12105/26264
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	ES TRPY447 2021
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	BioMed Central (BMC)
publisher.none.fl_str_mv	BioMed Central (BMC)
dc.source.none.fl_str_mv	reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII)
instname_str	Instituto de Salud Carlos III (ISCIII)
reponame_str	Repisalud
collection	Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Evolution of pfhrp2 and pfhrp3 deletions in Equatorial Guinea between the pre- and post-RDT introduction

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