Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity
Three nanostructured vehicles are proposed as potential carriers for photosensitizers to be used in photodynamic therapy: spherical nanoparticles, hexahedral microparticles and cylindrical magnetic nanorods. A comparative study of their photodynamic properties was performed, and the influence of the...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/120777 |
| Acceso en línea: | https://hdl.handle.net/2445/120777 |
| Access Level: | acceso abierto |
| Palabra clave: | Materials nanoestructurats Fotosensibilització (Biologia) Nanostructured materials Biological photosensitization |
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Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activityAlea Reyes, María ElisaRodrigues, Ana Mafalda NunesSerrà i Ramos, AlbertMora Giménez, MargaritaSagristá Gratovil, M. LluïsaGonzález, AsensioDurán, SaraDuch, MartaPlaza, José AntonioVallés Giménez, ElisaRussell, David A.Pérez García, M. Lluïsa (Maria Lluïsa)Materials nanoestructuratsFotosensibilització (Biologia)Nanostructured materialsBiological photosensitizationThree nanostructured vehicles are proposed as potential carriers for photosensitizers to be used in photodynamic therapy: spherical nanoparticles, hexahedral microparticles and cylindrical magnetic nanorods. A comparative study of their photodynamic properties was performed, and the influence of their size and the amount of loaded porphyrin was considered to discuss their effects in the observed photodynamic activity. All the vehicles have a gold surface, allowing functionalization with a disulfidecontaining porphyrin as the photosensitizer, as well as with a PEG-containing thiol to improve their biocompatibility and water solubility. The activity of the porphyrin loaded in each vehicle was assessed through in vitro photocytotoxicity studies using HeLa cells. A synergic effect for the porphyrin toxicity was observed in all of the vehicles. The zinc-containing porphyrin showed better production of singlet oxygen, and proved more photocytotoxic both in solution and loaded in any of the vehicles. The magnetism of the nanorods allows targeting with a magnetic field, but causes their aggregation, hampering the porphyrin's activity. Microparticles showed lower cell internalization but their bigger size allowed a high porphyrin loading, which translated into high photocytotoxicity. The highest cell internalization and photocytotoxicity was observed for the porphyrin-loaded nanoparticles, suggesting that a smaller size is favored in cell uptake.Royal Society of Chemistry2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/120777Articles publicats en revistes (Ciència dels Materials i Química Física)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1039/c7ra01569kRSC Advances, 2017, vol. 7, num. 28, p. 16963-16976https://doi.org/10.1039/c7ra01569kcc-by (c) Alea Reyes, María E. et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1207772026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| title |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| spellingShingle |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity Alea Reyes, María Elisa Materials nanoestructurats Fotosensibilització (Biologia) Nanostructured materials Biological photosensitization |
| title_short |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| title_full |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| title_fullStr |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| title_full_unstemmed |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| title_sort |
Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| dc.creator.none.fl_str_mv |
Alea Reyes, María Elisa Rodrigues, Ana Mafalda Nunes Serrà i Ramos, Albert Mora Giménez, Margarita Sagristá Gratovil, M. Lluïsa González, Asensio Durán, Sara Duch, Marta Plaza, José Antonio Vallés Giménez, Elisa Russell, David A. Pérez García, M. Lluïsa (Maria Lluïsa) |
| author |
Alea Reyes, María Elisa |
| author_facet |
Alea Reyes, María Elisa Rodrigues, Ana Mafalda Nunes Serrà i Ramos, Albert Mora Giménez, Margarita Sagristá Gratovil, M. Lluïsa González, Asensio Durán, Sara Duch, Marta Plaza, José Antonio Vallés Giménez, Elisa Russell, David A. Pérez García, M. Lluïsa (Maria Lluïsa) |
| author_role |
author |
| author2 |
Rodrigues, Ana Mafalda Nunes Serrà i Ramos, Albert Mora Giménez, Margarita Sagristá Gratovil, M. Lluïsa González, Asensio Durán, Sara Duch, Marta Plaza, José Antonio Vallés Giménez, Elisa Russell, David A. Pérez García, M. Lluïsa (Maria Lluïsa) |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Materials nanoestructurats Fotosensibilització (Biologia) Nanostructured materials Biological photosensitization |
| topic |
Materials nanoestructurats Fotosensibilització (Biologia) Nanostructured materials Biological photosensitization |
| description |
Three nanostructured vehicles are proposed as potential carriers for photosensitizers to be used in photodynamic therapy: spherical nanoparticles, hexahedral microparticles and cylindrical magnetic nanorods. A comparative study of their photodynamic properties was performed, and the influence of their size and the amount of loaded porphyrin was considered to discuss their effects in the observed photodynamic activity. All the vehicles have a gold surface, allowing functionalization with a disulfidecontaining porphyrin as the photosensitizer, as well as with a PEG-containing thiol to improve their biocompatibility and water solubility. The activity of the porphyrin loaded in each vehicle was assessed through in vitro photocytotoxicity studies using HeLa cells. A synergic effect for the porphyrin toxicity was observed in all of the vehicles. The zinc-containing porphyrin showed better production of singlet oxygen, and proved more photocytotoxic both in solution and loaded in any of the vehicles. The magnetism of the nanorods allows targeting with a magnetic field, but causes their aggregation, hampering the porphyrin's activity. Microparticles showed lower cell internalization but their bigger size allowed a high porphyrin loading, which translated into high photocytotoxicity. The highest cell internalization and photocytotoxicity was observed for the porphyrin-loaded nanoparticles, suggesting that a smaller size is favored in cell uptake. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/120777 |
| url |
https://hdl.handle.net/2445/120777 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1039/c7ra01569k RSC Advances, 2017, vol. 7, num. 28, p. 16963-16976 https://doi.org/10.1039/c7ra01569k |
| dc.rights.none.fl_str_mv |
cc-by (c) Alea Reyes, María E. et al., 2017 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Alea Reyes, María E. et al., 2017 http://creativecommons.org/licenses/by/3.0/es |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
| publisher.none.fl_str_mv |
Royal Society of Chemistry |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Ciència dels Materials i Química Física) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
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Universidad de Barcelona |
| reponame_str |
Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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1869423774477058048 |
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15.300719 |