Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)

17 Páginas.-- 4 Tablas.-- 7 Figuras

Detalles Bibliográficos
Autores: Mapelli-Brahm, Ana, Sánchez, Rosario, Pan, Xue, Moreno-Pérez, Antonio J., Garcés Mancheño, Rafael, Martínez-Force, Enrique, Weselake, Randall J., Salas, Joaquín J., Venegas-Calerón, Mónica
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/211673
Acceso en línea:http://hdl.handle.net/10261/211673
Access Level:acceso abierto
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spelling Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)Mapelli-Brahm, AnaSánchez, RosarioPan, XueMoreno-Pérez, Antonio J.Garcés Mancheño, RafaelMartínez-Force, EnriqueWeselake, Randall J.Salas, Joaquín J.Venegas-Calerón, Mónica17 Páginas.-- 4 Tablas.-- 7 FigurasLysophosphatidylcholine acyltransferase (LPCAT, EC 2.3.1.23) is an evolutionarily conserved key enzyme in the Lands cycle that catalyzes acylation of lysophosphatidylcholine (LPC) to produce phosphatidylcholine (PC), the main phospholipid in cellular membranes. In this study, three LPCAT genes from sunflower were identified and the corresponding proteins characterized. These HaLPCAT genes encoded functionally active enzymes that were able to complement a deficient yeast mutant. Moreover, enzymatic assays were carried out using microsomal preparations of the yeast cells. When acyl specificities were measured in the forward reaction, these enzymes exhibited a substrate preference for unsaturated acyl-CoAs, especially for linolenoyl-CoA, while in the reverse reaction, linoleoyl or linolenoyl acyl groups were transferred from PC to acyl-CoA to a similar extent. Expression levels of LPCAT genes were studied revealing distinct tissue-specific expression patterns. In summary, this study suggests that the combined forward and reverse reactions catalyzed by sunflower LPCATs facilitate acyl-exchange between the sn-2 position of PC and the acyl-CoA pool. Sunflower LPCATs displayed different characteristics, which could point to different functionalities, favoring the enrichment of seed triacylglycerols (TAGs) with polyunsaturated fatty acid (PUFA).This work was funded by the AEI and FEDER Project AGL2017-83449-R. RW is grateful for the support provided the Canada Research Chairs program and a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (RGPIN-2014-04585).Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/211673reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-83449-Rhttp://dx.doi.org/10.3389/fpls.2020.00403Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2116732026-05-22T06:33:51Z
dc.title.none.fl_str_mv Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
title Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
spellingShingle Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
Mapelli-Brahm, Ana
title_short Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
title_full Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
title_fullStr Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
title_full_unstemmed Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
title_sort Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
dc.creator.none.fl_str_mv Mapelli-Brahm, Ana
Sánchez, Rosario
Pan, Xue
Moreno-Pérez, Antonio J.
Garcés Mancheño, Rafael
Martínez-Force, Enrique
Weselake, Randall J.
Salas, Joaquín J.
Venegas-Calerón, Mónica
author Mapelli-Brahm, Ana
author_facet Mapelli-Brahm, Ana
Sánchez, Rosario
Pan, Xue
Moreno-Pérez, Antonio J.
Garcés Mancheño, Rafael
Martínez-Force, Enrique
Weselake, Randall J.
Salas, Joaquín J.
Venegas-Calerón, Mónica
author_role author
author2 Sánchez, Rosario
Pan, Xue
Moreno-Pérez, Antonio J.
Garcés Mancheño, Rafael
Martínez-Force, Enrique
Weselake, Randall J.
Salas, Joaquín J.
Venegas-Calerón, Mónica
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 17 Páginas.-- 4 Tablas.-- 7 Figuras
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/211673
url http://hdl.handle.net/10261/211673
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-83449-R
http://dx.doi.org/10.3389/fpls.2020.00403
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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