Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration

Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a n...

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Detalhes bibliográficos
Autores: Ripoll, Vanessa, Ladero Galán, Miguel, Santos Mazorra, Victoria Eugenia
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/4676
Acesso em linha:https://hdl.handle.net/20.500.14352/4676
Access Level:acceso abierto
Palavra-chave:66.0
2
3-butanediol
Resting cells
Raw glycerol
Kinetic modelling
Ingeniería química
3303 Ingeniería y Tecnología Químicas
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oai_identifier_str oai:docta.ucm.es:20.500.14352/4676
network_acronym_str ES
network_name_str España
repository_id_str
spelling Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentrationRipoll, VanessaLadero Galán, MiguelSantos Mazorra, Victoria Eugenia66.023-butanediolResting cellsRaw glycerolKinetic modellingIngeniería química3303 Ingeniería y Tecnología QuímicasBiodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess was developed and it is able to describe both the evolution of concentrations of relevant components with time and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was 0.034 g2,3-BDO/gX·h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was 2.52 gGly/g2,3-BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which provides new opportunities for developing and scaling-up glycerol biorefineries.ElsevierUniversidad Complutense de Madrid20212021-08-1420212021-08-14journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/4676reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/46762026-06-02T12:44:21Z
dc.title.none.fl_str_mv Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
title Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
spellingShingle Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
Ripoll, Vanessa
66.0
2
3-butanediol
Resting cells
Raw glycerol
Kinetic modelling
Ingeniería química
3303 Ingeniería y Tecnología Químicas
title_short Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
title_full Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
title_fullStr Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
title_full_unstemmed Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
title_sort Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration
dc.creator.none.fl_str_mv Ripoll, Vanessa
Ladero Galán, Miguel
Santos Mazorra, Victoria Eugenia
author Ripoll, Vanessa
author_facet Ripoll, Vanessa
Ladero Galán, Miguel
Santos Mazorra, Victoria Eugenia
author_role author
author2 Ladero Galán, Miguel
Santos Mazorra, Victoria Eugenia
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 66.0
2
3-butanediol
Resting cells
Raw glycerol
Kinetic modelling
Ingeniería química
3303 Ingeniería y Tecnología Químicas
topic 66.0
2
3-butanediol
Resting cells
Raw glycerol
Kinetic modelling
Ingeniería química
3303 Ingeniería y Tecnología Químicas
description Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess was developed and it is able to describe both the evolution of concentrations of relevant components with time and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was 0.034 g2,3-BDO/gX·h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was 2.52 gGly/g2,3-BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which provides new opportunities for developing and scaling-up glycerol biorefineries.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-08-14
2021
2021-08-14
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/4676
url https://hdl.handle.net/20.500.14352/4676
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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