Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid...

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Detalhes bibliográficos
Autores: Morales, Diego, Pris, Adriana J., Ruiz-Rodríguez, Alejandro, Soler-Rivas, Cristina
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/192513
Acesso em linha:http://hdl.handle.net/10261/192513
Access Level:acceso abierto
Palavra-chave:Supercritical CO2 extraction
Shiitake mushrooms
Cholesterol
Antioxidant activity
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spelling Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction methodMorales, DiegoPris, Adriana J.Ruiz-Rodríguez, AlejandroSoler-Rivas, CristinaSupercritical CO2 extractionShiitake mushroomsCholesterolAntioxidant activityThree extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid extraction (SFE) and remaining residue submitted to hot water extraction. Sequential design allowed reutilization of the nonextracted material as raw material for the successive extractions increasing extraction yields and separating interesting compounds. Obtained fractions contained different amounts of ß-glucans, chitins, eritadenine, lenthionine, ergosterol, proteins/peptides and phenolic compounds conferring them different bioactivities. Water soluble fractions showed high antioxidant activities (ABTS+• and DPPH• scavenging capacity and reducing power), they were also able to inhibit one of the main enzymes involved in hypertension (angiotensin-I converting enzyme) and the key enzyme of cholesterol metabolism (3-hydroxy-3-methylglutaryl coenzyme A reductase). The latter inhibitory activity was also noticed in SFE extracts although ergosterol and other lipid-like molecules were isolated. Dietary fibers were separated in the third extraction. Therefore, with this sequential extraction procedure bioactive compounds against CVDs can be selectively separated from a single batch of shiitake powder.The research was supported by AGL2014–56211 national R 1 D program from the Spanish Ministry of Science and Innovation and S2013/ABI-2728 regional program from the Community of Madrid (Spain).Peer reviewedWiley-VCHMinisterio de Economía y Competitividad (España)Comunidad de MadridMorales, Diego [0000-0002-4600-6673]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/192513reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#S2013/ABI-2728/ALIBIRD-CMinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014–56211-Rhttps://doi.org/10.1002/btpr.2616Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1925132026-05-22T06:33:51Z
dc.title.none.fl_str_mv Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
title Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
spellingShingle Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
Morales, Diego
Supercritical CO2 extraction
Shiitake mushrooms
Cholesterol
Antioxidant activity
title_short Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
title_full Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
title_fullStr Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
title_full_unstemmed Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
title_sort Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
dc.creator.none.fl_str_mv Morales, Diego
Pris, Adriana J.
Ruiz-Rodríguez, Alejandro
Soler-Rivas, Cristina
author Morales, Diego
author_facet Morales, Diego
Pris, Adriana J.
Ruiz-Rodríguez, Alejandro
Soler-Rivas, Cristina
author_role author
author2 Pris, Adriana J.
Ruiz-Rodríguez, Alejandro
Soler-Rivas, Cristina
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Morales, Diego [0000-0002-4600-6673]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Supercritical CO2 extraction
Shiitake mushrooms
Cholesterol
Antioxidant activity
topic Supercritical CO2 extraction
Shiitake mushrooms
Cholesterol
Antioxidant activity
description Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid extraction (SFE) and remaining residue submitted to hot water extraction. Sequential design allowed reutilization of the nonextracted material as raw material for the successive extractions increasing extraction yields and separating interesting compounds. Obtained fractions contained different amounts of ß-glucans, chitins, eritadenine, lenthionine, ergosterol, proteins/peptides and phenolic compounds conferring them different bioactivities. Water soluble fractions showed high antioxidant activities (ABTS+• and DPPH• scavenging capacity and reducing power), they were also able to inhibit one of the main enzymes involved in hypertension (angiotensin-I converting enzyme) and the key enzyme of cholesterol metabolism (3-hydroxy-3-methylglutaryl coenzyme A reductase). The latter inhibitory activity was also noticed in SFE extracts although ergosterol and other lipid-like molecules were isolated. Dietary fibers were separated in the third extraction. Therefore, with this sequential extraction procedure bioactive compounds against CVDs can be selectively separated from a single batch of shiitake powder.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/192513
url http://hdl.handle.net/10261/192513
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
S2013/ABI-2728/ALIBIRD-CM
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014–56211-R
https://doi.org/10.1002/btpr.2616
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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