A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams

Data on 74 dry-cured hams from Duroc × Iberian pigs were used to examine whether the tag polymorphism AY487830:g.2228T>C in the promoter region of the stearoyl-CoA desaturase [SCD] gene affect fat desaturation and monounsaturated fatty acid (MUFA) as previously described in purebred Duroc hams. S...

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Autores: Henriquez Rodriguez, Eliana, Tor i Naudí, Marc, Pena i Subirà, Ramona Natacha, Estany Illa, Joan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/59690
Acceso en línea:https://doi.org/10.5424/sjar/2015134-8323
http://hdl.handle.net/10459.1/59690
Access Level:acceso abierto
Palabra clave:Dry-cured ham
Fatty acid composition
Genetic marker
Meat quality
Pigs
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spelling A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hamsHenriquez Rodriguez, ElianaTor i Naudí, MarcPena i Subirà, Ramona NatachaEstany Illa, JoanDry-cured hamFatty acid compositionGenetic markerMeat qualityPigsData on 74 dry-cured hams from Duroc × Iberian pigs were used to examine whether the tag polymorphism AY487830:g.2228T>C in the promoter region of the stearoyl-CoA desaturase [SCD] gene affect fat desaturation and monounsaturated fatty acid (MUFA) as previously described in purebred Duroc hams. Samples were taken from sliced trays of dry-cured hams marketed as Jamón Ibérico de cebo, which were randomly purchased from the same supplier in different stores of the same supermarket chain. Genomic DNA was isolated from each sample to genotype for SCD and gender. Also, a sample of two slices was used to determine fat content and fatty acid (FA) composition by gas chromatography. The effect of the genotype (TT and CT) and gender (barrows and gilts) was estimated under a Bayesian setting. Results showed that the SCD polymorphism was associated to fat composition but not to fat content, with TT hams showing increased C18:1n-7, C18:1n-9, C20:1n-9 and MUFA (probability between 0.92-0.98) and decreased C18:2n-6, C20:4n-6 and polyunsaturated FA (PUFA) (probability between 0.91-0.99) as compared to the CT. As a result, the TT hams had more MUFA (0.95%) and a higher MUFA/PUFA ratio (0.43) than the CT. Barrows had more saturated FA (SFA) and less PUFA than gilts. No differences in MUFA content were found between genders. The SCD polymorphism had a greater impact on MUFA than using hams from barrows instead of gilts. It is concluded that the SCD polymorphism is a good tool to increase MUFA and MUFA/PUFA ratio in Duroc crossbred dry-cured hams.Funding: Spanish Ministry of Economy and Competitiveness (MINECO, grant AGL2012-33529). EHR is recipient of a PhD scholarship from the University of LleidaInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.5424/sjar/2015134-8323http://hdl.handle.net/10459.1/59690reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/MINECO//AGL2012-33529Reproducció del document publicat a https://doi.org/10.5424/sjar/2015134-8323Spanish Journal of Agricultural Research, 2015. vol. 13, núm. 4, p. e0404 (8)cc-by-nc (c) INIA, 2015info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/oai:repositori.udl.cat:10459.1/596902026-06-24T12:42:17Z
dc.title.none.fl_str_mv A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
title A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
spellingShingle A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
Henriquez Rodriguez, Eliana
Dry-cured ham
Fatty acid composition
Genetic marker
Meat quality
Pigs
title_short A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
title_full A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
title_fullStr A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
title_full_unstemmed A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
title_sort A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
dc.creator.none.fl_str_mv Henriquez Rodriguez, Eliana
Tor i Naudí, Marc
Pena i Subirà, Ramona Natacha
Estany Illa, Joan
author Henriquez Rodriguez, Eliana
author_facet Henriquez Rodriguez, Eliana
Tor i Naudí, Marc
Pena i Subirà, Ramona Natacha
Estany Illa, Joan
author_role author
author2 Tor i Naudí, Marc
Pena i Subirà, Ramona Natacha
Estany Illa, Joan
author2_role author
author
author
dc.subject.none.fl_str_mv Dry-cured ham
Fatty acid composition
Genetic marker
Meat quality
Pigs
topic Dry-cured ham
Fatty acid composition
Genetic marker
Meat quality
Pigs
description Data on 74 dry-cured hams from Duroc × Iberian pigs were used to examine whether the tag polymorphism AY487830:g.2228T>C in the promoter region of the stearoyl-CoA desaturase [SCD] gene affect fat desaturation and monounsaturated fatty acid (MUFA) as previously described in purebred Duroc hams. Samples were taken from sliced trays of dry-cured hams marketed as Jamón Ibérico de cebo, which were randomly purchased from the same supplier in different stores of the same supermarket chain. Genomic DNA was isolated from each sample to genotype for SCD and gender. Also, a sample of two slices was used to determine fat content and fatty acid (FA) composition by gas chromatography. The effect of the genotype (TT and CT) and gender (barrows and gilts) was estimated under a Bayesian setting. Results showed that the SCD polymorphism was associated to fat composition but not to fat content, with TT hams showing increased C18:1n-7, C18:1n-9, C20:1n-9 and MUFA (probability between 0.92-0.98) and decreased C18:2n-6, C20:4n-6 and polyunsaturated FA (PUFA) (probability between 0.91-0.99) as compared to the CT. As a result, the TT hams had more MUFA (0.95%) and a higher MUFA/PUFA ratio (0.43) than the CT. Barrows had more saturated FA (SFA) and less PUFA than gilts. No differences in MUFA content were found between genders. The SCD polymorphism had a greater impact on MUFA than using hams from barrows instead of gilts. It is concluded that the SCD polymorphism is a good tool to increase MUFA and MUFA/PUFA ratio in Duroc crossbred dry-cured hams.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.5424/sjar/2015134-8323
http://hdl.handle.net/10459.1/59690
url https://doi.org/10.5424/sjar/2015134-8323
http://hdl.handle.net/10459.1/59690
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//AGL2012-33529
Reproducció del document publicat a https://doi.org/10.5424/sjar/2015134-8323
Spanish Journal of Agricultural Research, 2015. vol. 13, núm. 4, p. e0404 (8)
dc.rights.none.fl_str_mv cc-by-nc (c) INIA, 2015
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/3.0/
rights_invalid_str_mv cc-by-nc (c) INIA, 2015
http://creativecommons.org/licenses/by-nc/3.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
publisher.none.fl_str_mv Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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