Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion

Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor respons...

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Autores: Faria, Melissa, Prats, Eva, Rosas Ramírez, Jonathan Ricardo, Bellot, Marina, Bedrossiantz, Juliette, Pagano, Maria, Valls, Arnau, Gómez-Canela, Cristian, Porta, Josep M, Mestres, Jordi, Garcia-Reyero, Natàlia, Faggio, Caterina, Gómez-Oliván, Leobardo Manuel, Raldúa, Demetrio
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Ramon Llull (URL)
Repositorio:DAU Arxiu Digital de la Universitat Ramon Llull
OAI Identifier:oai:dau.url.edu:20.500.14342/3643
Acceso en línea:http://hdl.handle.net/20.500.14342/3643
https://doi.org/10.1016/j.scitotenv.2021.145671
Access Level:acceso abierto
Palabra clave:Acetilcolinesterasa
Neurotoxicity
Predicted target profile
Acetylcholinesterase inhibitor
615
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spelling Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothionFaria, MelissaPrats, EvaRosas Ramírez, Jonathan RicardoBellot, MarinaBedrossiantz, JuliettePagano, MariaValls, ArnauGómez-Canela, CristianPorta, Josep MMestres, JordiGarcia-Reyero, NatàliaFaggio, CaterinaGómez-Oliván, Leobardo ManuelRaldúa, DemetrioAcetilcolinesterasaNeurotoxicityPredicted target profileAcetylcholinesterase inhibitor615Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities.info:eu-repo/semantics/publishedVersionElsevierUniversitat Ramon Llull. IQS202320232021info:eu-repo/semantics/article13 p.application/pdfhttp://hdl.handle.net/20.500.14342/3643https://doi.org/10.1016/j.scitotenv.2021.145671reponame:DAU Arxiu Digital de la Universitat Ramon Llullinstname:Universitat Ramon Llull (URL)InglésScience of the Total Environmentinfo:eu-repo/grantAgreement/MICINN i FEDER/PN I+D/CTM2017-83242-Rinfo:eu-repo/grantAgreement/DEC/SGR/2017 SGR_902info:eu-repo/grantAgreement/SUR del DEC/Beatriu de Pinós Programme/Grant No. 2016 BP 00233info:eu-repo/grantAgreement/MICINN i FSE/FI/PRE2018-083513info:eu-repo/grantAgreement/Sicily region i FSE/FI/Avviso 24© L'autor/aAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dau.url.edu:20.500.14342/36432026-06-21T06:40:37Z
dc.title.none.fl_str_mv Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
title Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
spellingShingle Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
Faria, Melissa
Acetilcolinesterasa
Neurotoxicity
Predicted target profile
Acetylcholinesterase inhibitor
615
title_short Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
title_full Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
title_fullStr Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
title_full_unstemmed Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
title_sort Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
dc.creator.none.fl_str_mv Faria, Melissa
Prats, Eva
Rosas Ramírez, Jonathan Ricardo
Bellot, Marina
Bedrossiantz, Juliette
Pagano, Maria
Valls, Arnau
Gómez-Canela, Cristian
Porta, Josep M
Mestres, Jordi
Garcia-Reyero, Natàlia
Faggio, Caterina
Gómez-Oliván, Leobardo Manuel
Raldúa, Demetrio
author Faria, Melissa
author_facet Faria, Melissa
Prats, Eva
Rosas Ramírez, Jonathan Ricardo
Bellot, Marina
Bedrossiantz, Juliette
Pagano, Maria
Valls, Arnau
Gómez-Canela, Cristian
Porta, Josep M
Mestres, Jordi
Garcia-Reyero, Natàlia
Faggio, Caterina
Gómez-Oliván, Leobardo Manuel
Raldúa, Demetrio
author_role author
author2 Prats, Eva
Rosas Ramírez, Jonathan Ricardo
Bellot, Marina
Bedrossiantz, Juliette
Pagano, Maria
Valls, Arnau
Gómez-Canela, Cristian
Porta, Josep M
Mestres, Jordi
Garcia-Reyero, Natàlia
Faggio, Caterina
Gómez-Oliván, Leobardo Manuel
Raldúa, Demetrio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Acetilcolinesterasa
Neurotoxicity
Predicted target profile
Acetylcholinesterase inhibitor
615
topic Acetilcolinesterasa
Neurotoxicity
Predicted target profile
Acetylcholinesterase inhibitor
615
description Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities.
publishDate 2021
dc.date.none.fl_str_mv 2021
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/3643
https://doi.org/10.1016/j.scitotenv.2021.145671
url http://hdl.handle.net/20.500.14342/3643
https://doi.org/10.1016/j.scitotenv.2021.145671
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Science of the Total Environment
info:eu-repo/grantAgreement/MICINN i FEDER/PN I+D/CTM2017-83242-R
info:eu-repo/grantAgreement/DEC/SGR/2017 SGR_902
info:eu-repo/grantAgreement/SUR del DEC/Beatriu de Pinós Programme/Grant No. 2016 BP 00233
info:eu-repo/grantAgreement/MICINN i FSE/FI/PRE2018-083513
info:eu-repo/grantAgreement/Sicily region i FSE/FI/Avviso 24
dc.rights.none.fl_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DAU Arxiu Digital de la Universitat Ramon Llull
instname:Universitat Ramon Llull (URL)
instname_str Universitat Ramon Llull (URL)
reponame_str DAU Arxiu Digital de la Universitat Ramon Llull
collection DAU Arxiu Digital de la Universitat Ramon Llull
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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