Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants

[EN]Background Long non-coding RNAs (lncRNAs) are involved in several immune processes, including the immune response to vaccination, but most of them remain uncharacterised in livestock species. The mechanism of action of aluminium adjuvants as vaccine components is neither not fully understood. Re...

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Autores: Bilbao Arribas, Martín, Varela Martínez, Endika, Abendaño Carbajo, Naiara, De Andrés, Damián, Luján, Lluís, Jugo Orrantia, Begoña Marina
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/54215
Acceso en línea:http://hdl.handle.net/10810/54215
Access Level:acceso abierto
Palabra clave:lncRNAs
ovis aries
vaccine
adjuvants
aluminium
systems biology
immune response
co-expression
RNA-seq
id ES_ee1fd35f8bfb9ae1a2db2f06ff32133f
oai_identifier_str oai:addi.ehu.eus:10810/54215
network_acronym_str ES
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repository_id_str
spelling Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvantsBilbao Arribas, MartínVarela Martínez, EndikaAbendaño Carbajo, NaiaraDe Andrés, DamiánLuján, LluísJugo Orrantia, Begoña MarinalncRNAsovis ariesvaccineadjuvantsaluminiumsystems biologyimmune responseco-expressionRNA-seq[EN]Background Long non-coding RNAs (lncRNAs) are involved in several immune processes, including the immune response to vaccination, but most of them remain uncharacterised in livestock species. The mechanism of action of aluminium adjuvants as vaccine components is neither not fully understood. Results We built a transcriptome from sheep PBMCs RNA-seq data in order to identify unannotated lncRNAs and analysed their expression patterns along protein coding genes. We found 2284 novel lncRNAs and assessed their conservation in terms of sequence and synteny. Differential expression analysis performed between animals inoculated with commercial vaccines or aluminium adjuvant alone and the co-expression analysis revealed lncRNAs related to the immune response to vaccines and adjuvants. A group of co-expressed genes enriched in cytokine signalling and production highlighted the differences between different treatments. A number of differentially expressed lncRNAs were correlated with a divergently located protein-coding gene, such as the OSM cytokine. Other lncRNAs were predicted to act as sponges of miRNAs involved in immune response regulation. Conclusions This work enlarges the lncRNA catalogue in sheep and puts an accent on their involvement in the immune response to repetitive vaccination, providing a basis for further characterisation of the non-coding sheep transcriptome within different immune cells.This research was supported by the Spanish Ministry of Economy and Industry project number AGL2013-49137-C3 to BMJ and University of the Basque Country (UPV/EHU) predoctoral fellowships to MB-A (PIF17/306) and EV-M (PIF15/361) and a postdoctoral fellowship to NA (ESP-COC16/43).BMC202120212021info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/54215reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICINN/AGL2013-49137-C3/https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08086-zinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Atribución 3.0 Españaoai:addi.ehu.eus:10810/542152026-06-18T09:23:17Z
dc.title.none.fl_str_mv Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
title Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
spellingShingle Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
Bilbao Arribas, Martín
lncRNAs
ovis aries
vaccine
adjuvants
aluminium
systems biology
immune response
co-expression
RNA-seq
title_short Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
title_full Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
title_fullStr Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
title_full_unstemmed Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
title_sort Identification of sheep lncRNAs related to the immune response to vaccines and aluminium adjuvants
dc.creator.none.fl_str_mv Bilbao Arribas, Martín
Varela Martínez, Endika
Abendaño Carbajo, Naiara
De Andrés, Damián
Luján, Lluís
Jugo Orrantia, Begoña Marina
author Bilbao Arribas, Martín
author_facet Bilbao Arribas, Martín
Varela Martínez, Endika
Abendaño Carbajo, Naiara
De Andrés, Damián
Luján, Lluís
Jugo Orrantia, Begoña Marina
author_role author
author2 Varela Martínez, Endika
Abendaño Carbajo, Naiara
De Andrés, Damián
Luján, Lluís
Jugo Orrantia, Begoña Marina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv lncRNAs
ovis aries
vaccine
adjuvants
aluminium
systems biology
immune response
co-expression
RNA-seq
topic lncRNAs
ovis aries
vaccine
adjuvants
aluminium
systems biology
immune response
co-expression
RNA-seq
description [EN]Background Long non-coding RNAs (lncRNAs) are involved in several immune processes, including the immune response to vaccination, but most of them remain uncharacterised in livestock species. The mechanism of action of aluminium adjuvants as vaccine components is neither not fully understood. Results We built a transcriptome from sheep PBMCs RNA-seq data in order to identify unannotated lncRNAs and analysed their expression patterns along protein coding genes. We found 2284 novel lncRNAs and assessed their conservation in terms of sequence and synteny. Differential expression analysis performed between animals inoculated with commercial vaccines or aluminium adjuvant alone and the co-expression analysis revealed lncRNAs related to the immune response to vaccines and adjuvants. A group of co-expressed genes enriched in cytokine signalling and production highlighted the differences between different treatments. A number of differentially expressed lncRNAs were correlated with a divergently located protein-coding gene, such as the OSM cytokine. Other lncRNAs were predicted to act as sponges of miRNAs involved in immune response regulation. Conclusions This work enlarges the lncRNA catalogue in sheep and puts an accent on their involvement in the immune response to repetitive vaccination, providing a basis for further characterisation of the non-coding sheep transcriptome within different immune cells.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/54215
url http://hdl.handle.net/10810/54215
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/AGL2013-49137-C3/
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08086-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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