Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis

34 Pags.- 9 Figs. The definitive version is available at: https://onlinelibrary.wiley.com/journal/13653040

Detalles Bibliográficos
Autores: Schneider, Sebastian, Schintlmeister, Arno, Becana Ausejo, Manuel, Wagner, Michael, Woebken, Dagmar, Wienkoop, Stefanie
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/182387
Acceso en línea:http://hdl.handle.net/10261/182387
Access Level:acceso abierto
Palabra clave:legume nodules
nanoSIMS
nitrogen fixation
stable isotope labeling
sulfur deficiency
symbiotic sulfate transporter (SST1)
id ES_ee1b2ff0a650fa3abf7a58a0e4ef1a15
oai_identifier_str oai:digital.csic.es:10261/182387
network_acronym_str ES
network_name_str España
repository_id_str
spelling Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesisSchneider, SebastianSchintlmeister, ArnoBecana Ausejo, ManuelWagner, MichaelWoebken, DagmarWienkoop, Stefanielegume nodulesnanoSIMSnitrogen fixationstable isotope labelingsulfur deficiencysymbiotic sulfate transporter (SST1)34 Pags.- 9 Figs. The definitive version is available at: https://onlinelibrary.wiley.com/journal/13653040Legume–rhizobia symbioses play a major role in food production for an ever growing human population. In this symbiosis, dinitrogen is reduced (“fixed”) to ammonia by the rhizobial nitrogenase enzyme complex and is secreted to the plant host cells, whereas dicarboxylic acids derived from photosynthetically produced sucrose are transported into the symbiosomes and serve as respiratory substrates for the bacteroids. The symbiosome membrane contains high levels of SST1 protein, a sulfate transporter. Sulfate is an essential nutrient for all living organisms, but its importance for symbiotic nitrogen fixation and nodule metabolism has long been underestimated. Using chemical imaging, we demonstrate that the bacteroids take up 20‐fold more sulfate than the nodule host cells. Furthermore, we show that nitrogenase biosynthesis relies on high levels of imported sulfate, making sulfur as essential as carbon for the regulation and functioning of symbiotic nitrogen fixation. Our findings thus establish the importance of sulfate and its active transport for the plant–microbe interaction that is most relevant for agriculture and soil fertility.This work was funded by the Austrian Science Fund (DK plus, W 1257‐820) and COST action FA1306 to S.W. and by grant AGL2017‐85775‐R from Ministerio de Economía y Competitividad‐Fondos Europeos de Desarrollo Regional (Spain) to M.B.Peer reviewedJohn Wiley & SonsAustrian Science FundEuropean CommissionMinisterio de Economía y Competitividad (España)Becana Ausejo, Manuel [0000-0002-1083-0804]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/182387reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1111/pce.13481Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1823872026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
title Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
spellingShingle Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
Schneider, Sebastian
legume nodules
nanoSIMS
nitrogen fixation
stable isotope labeling
sulfur deficiency
symbiotic sulfate transporter (SST1)
title_short Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
title_full Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
title_fullStr Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
title_full_unstemmed Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
title_sort Sulfate is transported at significant rates through the symbiosome membrane and is crucial for nitrogenase biosynthesis
dc.creator.none.fl_str_mv Schneider, Sebastian
Schintlmeister, Arno
Becana Ausejo, Manuel
Wagner, Michael
Woebken, Dagmar
Wienkoop, Stefanie
author Schneider, Sebastian
author_facet Schneider, Sebastian
Schintlmeister, Arno
Becana Ausejo, Manuel
Wagner, Michael
Woebken, Dagmar
Wienkoop, Stefanie
author_role author
author2 Schintlmeister, Arno
Becana Ausejo, Manuel
Wagner, Michael
Woebken, Dagmar
Wienkoop, Stefanie
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Austrian Science Fund
European Commission
Ministerio de Economía y Competitividad (España)
Becana Ausejo, Manuel [0000-0002-1083-0804]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv legume nodules
nanoSIMS
nitrogen fixation
stable isotope labeling
sulfur deficiency
symbiotic sulfate transporter (SST1)
topic legume nodules
nanoSIMS
nitrogen fixation
stable isotope labeling
sulfur deficiency
symbiotic sulfate transporter (SST1)
description 34 Pags.- 9 Figs. The definitive version is available at: https://onlinelibrary.wiley.com/journal/13653040
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/182387
url http://hdl.handle.net/10261/182387
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1111/pce.13481
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423614841847808
score 15,812429

Similares en LA Referencia