An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells

https://www.sciencedirect.com/science/article/pii/S002196731930247X?via%3Dihub

Detalles Bibliográficos
Autores: Bernardo Bermejo, Samuel|||0000-0001-9126-7453, Sánchez López, Elena, Castro Puyana, María|||0000-0003-1412-4103, Benito Martínez, Selma|||0000-0002-3118-0564, Lucio Cazaña, Francisco Javier de|||0000-0001-9249-8501, Marina Alegre, María Luisa|||0000-0002-5583-1624
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/39807
Acceso en línea:http://hdl.handle.net/10017/39807
https://dx.doi.org/10.1016/j.chroma.2019.03.009
Access Level:acceso abierto
Palabra clave:Diabetic nephropathy
HK-2 cells
Liquid chromatography-mass spectrometry
Metabolomics
Multivariate analysis
Química
Chemistry
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spelling An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cellsBernardo Bermejo, Samuel|||0000-0001-9126-7453Sánchez López, ElenaCastro Puyana, María|||0000-0003-1412-4103Benito Martínez, Selma|||0000-0002-3118-0564Lucio Cazaña, Francisco Javier de|||0000-0001-9249-8501Marina Alegre, María Luisa|||0000-0002-5583-1624Diabetic nephropathyHK-2 cellsLiquid chromatography-mass spectrometryMetabolomicsMultivariate analysisQuímicaChemistryhttps://www.sciencedirect.com/science/article/pii/S002196731930247X?via%3DihubDiabetes mellitus is a major health concern nowadays. It is estimated that 40 % of diabetics are affected by diabetic nephropathy, one of the complications derived from high glucose blood levels which can lead to chronic loss of kidney function. It is now clear that the renal proximal tubule plays a critical role in the progression of diabetic nephropathy but research focused on studying the molecular mechanisms involved is still needed. The aim of this work was to develop a liquid chromatography-mass spectrometry platform to carry out, for the first time, the untargeted metabolomic analysis of high glucose-induced changes in cultured human proximal tubular HK-2 cells. In order to find the metabolites which were affected by high glucose and to expand the metabolite coverage, intra- and extracellular fluid from HK-2 cells exposed to high glucose (25 mM), normal glucose (5.5 mM) or osmotic control (5.5 mM glucose + 19.5 mM mannitol) were analyzed by two complementary chromatographic modes: hydrophilic interaction and reversed-phase liquid chromatography. Non-supervised principal components analysis showed a good distribution among the three groups of samples. Statistically significant variables were chosen for further metabolite identification. Different metabolic pathways were affected mainly those derived from amino acidic, polyol, and nitrogenous bases metabolism.Ministerio de Economía y Competitividad20192019-07-05journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/39807https://dx.doi.org/10.1016/j.chroma.2019.03.009reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2016-76368-P Nuevos nanoaditivos para separación quiral por electroforesis capilar: aplicación a la determinación de biomarcadoresopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/398072026-06-18T11:13:07Z
dc.title.none.fl_str_mv An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
title An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
spellingShingle An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
Bernardo Bermejo, Samuel|||0000-0001-9126-7453
Diabetic nephropathy
HK-2 cells
Liquid chromatography-mass spectrometry
Metabolomics
Multivariate analysis
Química
Chemistry
title_short An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
title_full An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
title_fullStr An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
title_full_unstemmed An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
title_sort An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
dc.creator.none.fl_str_mv Bernardo Bermejo, Samuel|||0000-0001-9126-7453
Sánchez López, Elena
Castro Puyana, María|||0000-0003-1412-4103
Benito Martínez, Selma|||0000-0002-3118-0564
Lucio Cazaña, Francisco Javier de|||0000-0001-9249-8501
Marina Alegre, María Luisa|||0000-0002-5583-1624
author Bernardo Bermejo, Samuel|||0000-0001-9126-7453
author_facet Bernardo Bermejo, Samuel|||0000-0001-9126-7453
Sánchez López, Elena
Castro Puyana, María|||0000-0003-1412-4103
Benito Martínez, Selma|||0000-0002-3118-0564
Lucio Cazaña, Francisco Javier de|||0000-0001-9249-8501
Marina Alegre, María Luisa|||0000-0002-5583-1624
author_role author
author2 Sánchez López, Elena
Castro Puyana, María|||0000-0003-1412-4103
Benito Martínez, Selma|||0000-0002-3118-0564
Lucio Cazaña, Francisco Javier de|||0000-0001-9249-8501
Marina Alegre, María Luisa|||0000-0002-5583-1624
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Diabetic nephropathy
HK-2 cells
Liquid chromatography-mass spectrometry
Metabolomics
Multivariate analysis
Química
Chemistry
topic Diabetic nephropathy
HK-2 cells
Liquid chromatography-mass spectrometry
Metabolomics
Multivariate analysis
Química
Chemistry
description https://www.sciencedirect.com/science/article/pii/S002196731930247X?via%3Dihub
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-07-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/39807
https://dx.doi.org/10.1016/j.chroma.2019.03.009
url http://hdl.handle.net/10017/39807
https://dx.doi.org/10.1016/j.chroma.2019.03.009
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2016-76368-P Nuevos nanoaditivos para separación quiral por electroforesis capilar: aplicación a la determinación de biomarcadores
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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