In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone

Current knowledge on the sensitivity of marine fish to androgenic environmental chemicals is limited, despite the growing interest in the effects of endocrine disrupting chemicals. To study in vivo the effects of testosterone (T) on the fish immune response, we used a microencapsulation implant tech...

Full description

Bibliographic Details
Authors: Castillo-Briceño, Patricia, Águila-Martínez, S., Liarte, S., García-Alcázar, Alicia, Meseguer, José, Mulero, Victoriano, García-Ayala, Alfonsa
Format: article
Publication Date:2013
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/313685
Online Access:http://hdl.handle.net/10261/313685
Access Level:Open access
Keyword:Centro Oceanográfico de Murcia
Acuicultura
id ES_ee0833dcdadef87bbb9da8b9a5d82229
oai_identifier_str oai:digital.csic.es:10261/313685
network_acronym_str ES
network_name_str España
repository_id_str
spelling In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosteroneCastillo-Briceño, PatriciaÁguila-Martínez, S.Liarte, S.García-Alcázar, AliciaMeseguer, JoséMulero, VictorianoGarcía-Ayala, AlfonsaCentro Oceanográfico de MurciaAcuiculturaCurrent knowledge on the sensitivity of marine fish to androgenic environmental chemicals is limited, despite the growing interest in the effects of endocrine disrupting chemicals. To study in vivo the effects of testosterone (T) on the fish immune response, we used a microencapsulation implant technique, the in situ forming microparticle system, containing 1 mg T/kg body weight (T-ISM), in adult specimens of gilthead seabream (Sparus aurata L.), a species of great economic interest. We demonstrated that implants themselves (without T) have no significant effect on most of the parameters measured. In T-ISM implanted fish, T serum levels reached supraphysiological concentrations accompanied by a slight increase in 11-ketotestosterone and 17β-estradiol levels 21 days post-implantation (dpi). Liver and head-kidney samples were processed 7 and 21 dpi to assess T-ISM effect on (i) the mRNA expression of genes involved in the metabolism of steroid hormones and in the immune response, and (ii) phagocyte activities. The expression profile of cytokines, chemokines and immune receptors was altered in T-ISM implanted animals that showed an early pro-inflammatory tendency, and then, a mixed pro-/anti-inflammatory activation during longer exposure. Furthermore, the enhancement of phagocytic activity and the production of reactive oxygen species by leukocytes 21 dpi in T-ISM implanted specimens suggest fine modulation of the innate immune response by T. Taken together, these data demonstrate for the first time the feasibility of using ISM implants in an aquatic species, and provide new data on the role played by T on the immune response in fish.Sí202320232013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/313685reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Murciahttps://doi.org/10.1016/j.steroids.2012.10.013info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3136852026-05-22T06:33:51Z
dc.title.none.fl_str_mv In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
title In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
spellingShingle In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
Castillo-Briceño, Patricia
Centro Oceanográfico de Murcia
Acuicultura
title_short In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
title_full In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
title_fullStr In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
title_full_unstemmed In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
title_sort In situ forming microparticle implants for delivery of sex steroids in fish:Modulation of the immune response of gilthead seabream by testosterone
dc.creator.none.fl_str_mv Castillo-Briceño, Patricia
Águila-Martínez, S.
Liarte, S.
García-Alcázar, Alicia
Meseguer, José
Mulero, Victoriano
García-Ayala, Alfonsa
author Castillo-Briceño, Patricia
author_facet Castillo-Briceño, Patricia
Águila-Martínez, S.
Liarte, S.
García-Alcázar, Alicia
Meseguer, José
Mulero, Victoriano
García-Ayala, Alfonsa
author_role author
author2 Águila-Martínez, S.
Liarte, S.
García-Alcázar, Alicia
Meseguer, José
Mulero, Victoriano
García-Ayala, Alfonsa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Centro Oceanográfico de Murcia
Acuicultura
topic Centro Oceanográfico de Murcia
Acuicultura
description Current knowledge on the sensitivity of marine fish to androgenic environmental chemicals is limited, despite the growing interest in the effects of endocrine disrupting chemicals. To study in vivo the effects of testosterone (T) on the fish immune response, we used a microencapsulation implant technique, the in situ forming microparticle system, containing 1 mg T/kg body weight (T-ISM), in adult specimens of gilthead seabream (Sparus aurata L.), a species of great economic interest. We demonstrated that implants themselves (without T) have no significant effect on most of the parameters measured. In T-ISM implanted fish, T serum levels reached supraphysiological concentrations accompanied by a slight increase in 11-ketotestosterone and 17β-estradiol levels 21 days post-implantation (dpi). Liver and head-kidney samples were processed 7 and 21 dpi to assess T-ISM effect on (i) the mRNA expression of genes involved in the metabolism of steroid hormones and in the immune response, and (ii) phagocyte activities. The expression profile of cytokines, chemokines and immune receptors was altered in T-ISM implanted animals that showed an early pro-inflammatory tendency, and then, a mixed pro-/anti-inflammatory activation during longer exposure. Furthermore, the enhancement of phagocytic activity and the production of reactive oxygen species by leukocytes 21 dpi in T-ISM implanted specimens suggest fine modulation of the innate immune response by T. Taken together, these data demonstrate for the first time the feasibility of using ISM implants in an aquatic species, and provide new data on the role played by T on the immune response in fish.
publishDate 2013
dc.date.none.fl_str_mv 2013
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/313685
url http://hdl.handle.net/10261/313685
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Murcia
https://doi.org/10.1016/j.steroids.2012.10.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423582594990080
score 15,811543

Similar Items